A simple HPLC method for analysing diaminopimelic acid diastereomers in cell walls of Gram-positive bacteria

International Journal of Systematic and Evolutionary Microbiology

Yao

et al. 2016

A novel actinomycete, designated strain 3H-GS17 T , was isolated from the head of an ant (Lasius fuliginosus L.) and characterized using a polyphasic approach. 16S rRNA gene sequence similarity studies showed that strain 3H-GS17 T belongs to the genus Actinocorallia with high sequence similarity to Actinocorallia glomerata JCM 9376 T (98.13 %) and Actinocorallia longicatena JCM 9377 T (97.64 %). The chemotaxonomic properties of strain 3H-GS17 T were also consistent with those of members of the genus Actinocorallia. The cell wall contained mesodiaminopimelic acid and whole-cell sugars were ribose, mannose, glucose, galactose and madurose. The predominant menaquinones were MK-9(H 4 ), MK-9(H 6 ) and MK-9(H 8 ). The phospholipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The major fatty acids were C 16 : 0 and C 18 : 1 !7с. A combination of DNA-DNA hybridization experiments and phenotypic tests were carried out between strain 3H-GS17 T and its closely related strains, which further clarified their relatedness and demonstrated that 3H-GS17 T could be distinguished from these strains. Therefore, strain 3H-GS17 T is concluded to represent a novel species of the genus Actinocorallia, for which the name Actinocorallia lasiicapitis sp. nov. is proposed. The type strain is 3H-GS17 T (=DSM 100595 T =CGMCC 4.7282 T ).The genus Actinocorallia was first proposed by Iinuma et al. (1994) as a member of the family Thermomonosporaceae and its description was emended by Zhang et al. (2001). Members of the genus form spore chains on the aerial mycelium. The genus is characterized chemotaxonomically by the presence of meso-diaminopimelic acid in the cell wall, diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as the major phospholipids, MK-9 (H 4 ), MK-9(H 6 ) and MK-9(H 8 ) as the predominant menaquinones, and hexadecanoic, 14-methylpentadecanoic, octadecanoic and 19-methyloctadecanoic acids as the predominant fatty acids (Trujillo & Goodfellow, 2012). At the time of writing, the genus comprises seven species with validly published names (http://www.bacterio.net/actinocorallia.html). During a study of the diversity of symbiotic actinomycetes in ants, strain 3H-GS17 T was isolated from the head of Lasius fuliginosus L. In this study, we performed a polyphasic taxonomic analysis on this strain, and propose that it is a representative of a novel species of the genus Actinocorallia.Strain 3H-GS17 T was isolated from the head of L. fuliginosus L. collected from Northeast Agriculture University (Harbin, Heilongjiang, China). L. fuliginosus individuals were caught under a willow tree adjacent to their formicary. Ten individuals were surface-disinfected in 70 % ethanol for 60 s and then washed three times in sterile distilled water. Surface-disinfected individual ants were divided into head, mesosoma and gaster, and each body part was separately put in 500 µl of sterile water with shaking on a rotary shaker at 180 r.p.m at 28 C for ...

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confidence: 99%

Actinocorallia lasiicapitis sp. nov., an actinomycete isolated from the head of an ant (Lasius fuliginosus L.)

International Journal of Systematic and Evolutionary Microbiology

Yao

et al. 2016

“…The isomer of diaminopimelic acid in the cell-wall hydrolysates was derivatized according to McKerrow et al (2000) and analysed using an HPLC method according to Jia et al (2015). The whole-cell sugars were analysed according to the procedures developed by Lechevalier & Lechevalier (1980).…”

mentioning

confidence: 99%

Nonomuraea zeae sp. nov., isolated from the rhizosphere of corn (Zea mays L.)

Shen

Jia

International Journal of Systematic and Evolutionary Microbiology

et al. 2016

A novel actinobacterium, designated strain NEAU-ND5 T , was isolated from the rhizosphere of corn (Zea mays L.) collected in Heilongjiang Province, north-east China, and characterized using a polyphasic approach. 16S rRNA gene sequence analysis showed that strain NEAU-ND5 T was a member of the genus Nonomuraea, with highest sequence similarities to Nonomuraea jabiensis A4036 T (98.29 %), Nonomuraea rosea GW12687 T (98.25 %), Nonomuraea candida HMC10 T (98.22 %), Nonomuraea rhizophila YIM 67092 T (98.04 %) and Nonomuraea kuesteri NRRL B-24325 T (98.04 %). Similarities to other type strains of the genus Nonomuraea were lower than 98 %. Morphological and chemotaxonomic properties of strain NEAU-ND5 T were also consistent with the description of the genus Nonomuraea. The cell wall contained mesodiaminopimelic acid and the whole-cell sugars were glucose, ribose and madurose. The phospholipid profile consisted of diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, hydroxy-phosphatidylmonomethylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The major menaquinones were MK-9(H 4 ), MK-9(H 2 ) and MK-9(H 0 ). The predominant cellular fatty acids were iso-C 16:0 and 10-methyl C 17:0 . A combination of DNA-DNA hybridization results and some phenotypic characteristics demonstrated that strain NEAU-ND5 T was clearly distinguished from its closely related Nonomuraea species. Consequently, it is concluded that strain NEAU-ND5 T represents a novel species of the genus Nonomuraea, for which the name Nonomuraea zeae sp. nov. is proposed. The type strain is NEAU-ND5 T (=CGMCC 4.7280 T =DSM 100528 T ).

“…Cells were harvested by centrifugation, then washed with distilled water and freeze-dried. The isomer of diaminopimelic acid in the cell-wall hydrolysates was derivatized and analysed by a HPLC method (McKerrow et al, 2000). The whole-cell sugars were analysed according to the procedures developed by Lechevalier & Lechevalier (1980).…”

mentioning

confidence: 99%

Streptomyces polygonati sp. nov., an endophytic actinomycete isolated from a root of Polygonatum odoratum (Mill.)

Guo

International Journal of Systematic and Evolutionary Microbiology

et al. 2016

A novel actinomycete, designated strain NEAU-G9 T , was isolated from the root of Polygonatum odoratum (Mill.) collected from Harbin, Heilongjiang province, north China, and was characterized using a polyphasic approach. Key morphological and chemotaxonomic properties confirmed the affiliation of strain NEAU-G9 T to the genus Streptomyces. Strain NEAU-G9 T contained LL-diaminopimelic acid as the diamino acid. The predominant menaquinones were MK-9(H 8 ), MK-9(H 6 ) and MK-9(H 4 ). The phospholipid profile contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The predominant fatty acids were iso-C 16 : 0 , anteiso-C 15 : 0 and C 16 : 0 .16S rRNA gene sequence similarity studies showed that strain NEAU-G9 T belongs to the genus Streptomyces and exhibits the highest sequence similarity to Streptomyces yanglinensis JCM 13275 T (97.75 %). However, phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain NEAU-G9 T is most closely related to Streptomyces misakiensis JCM 4062 T (97.12 % sequence similarity). A combination of DNA-DNA hybridization results and some phenotypic characteristics indicated that strain NEAU-G9 T can be clearly distinguished from S. yanglinensis JCM 13275 T and S. misakiensis JCM 4062 T . Consequently, strain NEAU-G9 T represents a novel species of the genus Streptomyces, for which the name Streptomyces polygonati sp. nov. is proposed. The type strain is NEAU-G9 T (5CGMCC 4.7237 T 5DSM 100521 T ).