0 0 0 . A simple and sensitive method for separating and detecting the LL, DD and meso diastereomers of the dibasic amino acid diaminopimelic acid (DAP) in the peptidoglycan of Gram-positive bacteria is described.This method is based on reverse phase HPLC separation of chiral derivatives of DAP followed by £uorescence detection of the ophthaldehyde derivatives. Its application to the analyses of cell walls of several Gram-positive bacteria is described, where10 mg or less of dry cells is required.

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