A simple HPLC method for the determination of apigenin in mouse tissues

Abstract

The flavone apigenin occurs in many leafy vegetables and fruits. It has been reported to have cancer chemopreventive efficacy in rodents. An HPLC method described previously for the determination of tricin, the dimethoxy cogener of apigenin, was modified and validated for measurement of apigenin in mouse tissues. Separation was carried out on a Hypersil-BDS C(18) column (4.6 x 250 mm) with an isocratic mobile phase of 55% methanol in 0.1 m ammonium acetate, pH 5.10, containing 0.27 mm disodium ethylenediamine tetraacetic acid. UV detection was at 336 nm, without interference from endogenous tissue compounds. The assay was linear in the range 25-400 ng/mL, 0.25-4 microg/mL and 2.5-40 microg/mL, with r(2) > 0.99 in all cases, for mouse plasma, liver and intestinal mucosa, respectively. Apigenin in mouse plasma, liver and intestinal mucosa was efficiently extracted with 0.1 m acetic acid in acetone. The assay recovery at low, medium and high concentrations was between 94.6 and 131.7% for all biomatrices, with a relative standard deviation of <10%. The lower limit of quantification for plasma was 25 ng/mL with a relative standard deviation of <15%. The method was used to measure the steady-phase apigenin levels in tissues of mice receiving apigenin in their diet.

Tissue distribution in mice and metabolism in murine and human liver of apigenin and tricin, flavones with putative cancer chemopreventive properties

“…In mice exposed to flavones plasma levels of tricin were about 4.5-fold higher than those of apigenin (Table 1). In four out of ten mice on apigenin, plasma concentrations of the flavone were below the limit of quantitation (25 ng/mL, [9]), whilst tricin was measurable in all animals, which had received tricin. Consistent with the results observed in the plasma, hepatic and small intestinal levels of tricin exceeded those of apigenin by 33 and 100 %, respectively ( Table 1).…”

“…Flavone analysis by HPLC Analysis of tricin and apigenin in plasma, tissues and in incubation mixtures with liver or gut fractions was performed using a reversed phase HPLC method described previously [7][8][9]. Two systems were used: i) a Varian ProStar 230 pump, a ProStar 410 autosampler and a ProStar 325 UV-visible or ProStar 320 photodiode array detector (Varian Inc, Oxford, UK), or ii) an Acquity UPLC system (Waters Corp. Milford, USA) with TUV detector and sample and binary solvent manager.…”

Apigenin-Loaded PLGA-DMSA Nanoparticles: A Novel Strategy to Treat Melanoma Lung Metastasis

“…Blood samples were collected from the animals following the reported method . Samples from lung tissues were prepared as per the reported method and analyzed by HPLC as per the reported method . Both plasma and lung tissue drug concentrations were plotted against time, and PK parameters were determined by WinNonlin software (Certara, London, UK).…”

Design and development of tofacitinib citrate loaded transferosomal gel for skin cancer by box-Behnken design- doe approach

“…In comparison to liposomes, Fang et al discovered that adding the surfactant sodium deoxycholate to phosphatidylethanolamine vesicles significantly increased the entrapment efficacy of 5-aminolevulinic acid. Gupta et al discovered that transfersomes were substantially more successful in trapping than liposomes and niosomes 16 .…”