A simple identification method for vaginal secretions using relative quantification of Lactobacillus DNA

Doi, Miwako; Gamo, Shinsuke; Okiura, Tatsuyuki; Nishimukai, Hiroaki; Asano, Masaharu

doi:10.1016/j.fsigen.2014.05.005

Cited by 28 publications

(18 citation statements)

References 37 publications

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“…65 The microflora of the vagina has been exploited by several authors as an alternative tool to identify vaginal material and is described in the following text. [85][86][87][88][89] Menstrual blood is also a complex fluid composed of varying amounts of circulatory blood, vaginal secretions, microbial communities, and cells associated with the menstrual cycle. It is the remodeling of the endometrium during menstruation that offers potential candidates for identification.…”

Section: Rna-and Dna-based Technologiesmentioning

confidence: 99%

“…[128][129][130][131] The microflora of the vagina has been exploited by several authors as a tool to identify vaginal material. [85][86][87][88] A healthy human vagina is dominated by lactobacilli, and typically Lactobacillus crispatus, L. gasseri, L. jensenii, and/or L. iners are found in women. Usually one species predominates; for example, L. crispatus is prevalent among women in North America, Europe, and Asia.…”

Section: Microbial Community Profilingmentioning

confidence: 99%

See 1 more Smart Citation

Forensic body fluid identification: state of the art

Harbison¹,

Fleming²

2016

RRFMS

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Body fluid identification is a key component in the forensic scientists' tool box and has been carried out both at the crime scene and in the laboratory for many years. Historically, methods relied on (bio) chemical-based tests, many of which lacked specificity. In this review, current technologies for identifying body fluids are described including the use of RNA (mRNA and miRNA), epigenetics, spectroscopic techniques such as Raman spectroscopy and microspectrophotometry, biosensors, and immunochromatographic methods which are outlined alongside their strengths and weaknesses. The potential for new insights into the identification of cells from new technologies such as massively parallel sequencing is explored.

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Section: Rna-and Dna-based Technologiesmentioning

confidence: 99%

Section: Microbial Community Profilingmentioning

confidence: 99%

Forensic body fluid identification: state of the art

Harbison¹,

Fleming²

2016

RRFMS

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“…However, unlike hemoglobin in blood [3], prostate-specific antigen in Electronic supplementary material The online version of this article (doi:10.1007/s00216-015-8877-x) contains supplementary material, which is available to authorized users. semen [4], or statherin in saliva [5], no characteristic protein marker has been demonstrated for vaginal fluids, and an optimal method for identifying vaginal fluids has not been adequately established [6]. A method detecting cornulin and cornifin for discriminating vaginal fluids from other body fluids has been recently reported; however, this method involves time-consuming sample preparation for its use in practical forensic investigation, wherein rapidity is important.…”

Section: Introductionmentioning

confidence: 99%

Identification and evaluation of potential forensic marker proteins in vaginal fluid by liquid chromatography/mass spectrometry

2015

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Vaginal fluid is one of the most common body fluids found at crime scenes. Discriminating vaginal fluid from other body fluids is important in forensic science; however, few potential protein markers have been reported to date. Proteomic methods for identifying protein markers have gained attention, although few reports have applied this technology to forensic protein markers. Therefore, to identify characteristic vaginal proteins, we examined various body fluids (nasal secretions, saliva, urine, semen, vaginal fluids, and sweat) using liquid chromatography/electrospray ionization time-of-flight mass spectrometry and peptide mass fingerprinting. We identified three components (average molecular mass values 17,237 ± 2, 18,063 ± 2, and 15,075 ± 1) detectable only in vaginal samples: two human small proline-rich protein 3 (SPRR3) isoforms and a human fatty acid-binding protein 5 (FABP5) with an acetylated (+42) N-terminal region lacking the initiator methionine residue (-131). Using ELISA, these yielded markedly high average values in vaginal fluids. The mass spectra of these proteins were not detected in infant saliva but were detected in the vaginal fluid throughout the menstrual cycle. The results of forensic analysis (detection limit, mixed body fluid samples, casework samples, and blind samples) suggest that these proteins are potential forensic markers. In conclusion, high SPRR3 and FABP5 expression levels, which may be used as potential markers for vaginal fluid identification in forensic science, were detected in vaginal fluids from healthy adults.

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“…For example, Lactobacillus crispatus, Lactobacillus jensenii, and Atopobium vaginae have been associated with vaginal secretions, while Lactobacillus iners, Lactobacillus gasseri, and Gardnerella vaginalis have been found in other body fluids as well (56)(57)(58). Tissue specific to the vagina could be informative in rape cases.…”

Section: Microbial Forensics and Human Identity Testingmentioning

confidence: 99%

Expansion of Microbial Forensics

2016

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cMicrobial forensics has been defined as the discipline of applying scientific methods to the analysis of evidence related to bioterrorism, biocrimes, hoaxes, or the accidental release of a biological agent or toxin for attribution purposes. Over the past 15 years, technology, particularly massively parallel sequencing, and bioinformatics advances now allow the characterization of microorganisms for a variety of human forensic applications, such as human identification, body fluid characterization, postmortem interval estimation, and biocrimes involving tracking of infectious agents. Thus, microbial forensics should be more broadly described as the discipline of applying scientific methods to the analysis of microbial evidence in criminal and civil cases for investigative purposes.

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A simple identification method for vaginal secretions using relative quantification of Lactobacillus DNA

Cited by 28 publications

References 37 publications

Forensic body fluid identification: state of the art

Forensic body fluid identification: state of the art

Identification and evaluation of potential forensic marker proteins in vaginal fluid by liquid chromatography/mass spectrometry

Expansion of Microbial Forensics

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