A simple instrument to characterize convective and diffusive transport of single hollow fibers of short length

Bridge, M.J.; Broadhead, Kelly W.; Hitchcock, Robert W.; Webb, K. E.; Tresco, Patrick A.

doi:10.1016/s0376-7388(00)00584-6

Cited by 10 publications

(4 citation statements)

References 24 publications

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“…4). Potential explanations, which need to be investigated further, are, for example, an increased release of specific growth factors as a direct result from the shear stress or improved nutrient supply due to introducing active mass transfer by convection as previously demonstrated in hollow fiber membranes used for graft cell cultivation or blood cell oxygenators [38,39,40]. Overall, our data once more highlight the complex setting of skin physiology and are first indications that simple perfusion of skin models might be not sufficient to improve the skin barrier function.…”

Section: Discussionmentioning

confidence: 99%

Development of a Perfusion Platform for Dynamic Cultivation of in vitro Skin Models

Strüver¹,

Frieß²,

Hedtrich

2017

Skin Pharmacol Physiol

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Reconstructed skin models are suitable test systems for toxicity testing and for basic investigations on (patho-)physiological aspects of human skin. Reconstructed human skin, however, has clear limitations such as the lack of immune cells and a significantly weaker skin barrier function compared to native human skin. Potential reasons for the latter might be the lack of mechanical forces during skin model cultivation which is performed classically in static well-plate setups. Mechanical forces and shear stress have a major impact on tissue formation and, hence, tissue engineering. In the present work, a perfusion platform was developed allowing dynamic cultivation of in vitro skin models. The platform was designed to cultivate reconstructed skin at the air-liquid interface with a laminar and continuous medium flow below the dermis equivalent. Histological investigations confirmed the formation of a significantly thicker stratum corneum compared to the control cultivated under static conditions. Moreover, the skin differentiation markers involucrin and filaggrin as well as the tight junction proteins claudin 1 and occludin showed increased expression in the dynamically cultured skin models. Unexpectedly, despite improved differentiation, the skin barrier function of the dynamically cultivated skin models was not enhanced compared with the skin models cultivated under static conditions.

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Section: Discussionmentioning

confidence: 99%

Development of a Perfusion Platform for Dynamic Cultivation of in vitro Skin Models

Strüver¹,

Frieß²,

Hedtrich

2017

Skin Pharmacol Physiol

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“…Permeability measurements were carried out on the HFMs using a simplified version of a previously-reported method [33]. A chamber was constructed to allow countercurrent flow between the HFMs and the chamber interior.…”

Section: Methodsmentioning

confidence: 99%

Development and evaluation of elastomeric hollow fiber membranes as small diameter vascular graft substitutes

Mercado-Pagán

Kang

Findlay

et al. 2015

Materials Science and Engineering: C

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Engineering of small diameter (<6 mm) vascular grafts (SDVGs) for clinical use, remains a significant challenge. Here, elastomeric polyester urethane (PEU)-based hollow fiber membranes (HFM) are presented as an SDVG candidate to target the limitations of current technologies and improve tissue engineering designs. HFMs are fabricated by a simple phase inversion method. HFM dimensions are tailored through adjustments to fabrication parameters. The walls of HFMs are highly porous. The HFMs are very elastic, with moduli ranging from 1–4 MPa, strengths from 1–5 MPa, and max strains from 300–500%. Permeability of the HFMs varies from 0.5–3.5×10−6 cm/s, while burst pressure varies from 25 to 35 psi. The suture retention forces of HFMs are in the range of 0.8 to 1.2 N. These properties match those of blood vessels. A slow degradation profile is observed for all HFMs, with 71 to 78% of the original mass remaining after 8 weeks, providing a suitable profile for potential cellular incorporation and tissue replacement. Both human endothelial cells and human mesenchymal stem cells proliferate well in the presence of HFMs up to 7 days. These results demonstrate a promising customizable PEU HFMs for small diameter vascular repair and tissue engineering applications.

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“…Diffusive permeability was determined using a range of FITC‐labeled dextrans of varying molecular weights at 37°C as previously described 33. This measurement also served as a means to assess the homogeneity of the membranes.…”

Section: Methodsmentioning

confidence: 99%

Fabrication of permeable tubular constructs from chemically modified chitosan with enhanced antithrombogenic property

et al. 2009

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The failure of artificial vascular grafts in small diameter vessel replacement is mainly due to the early formation of thrombosis. To prevent the occurrence of thrombosis, much effort has been focused on developing an anti-thrombogenic coating of synthetic vascular prostheses or artificial conduits with improved anti-thrombogenic properties. Because surface coatings may be unstable for long-term applications, a bulk material with anti-thrombogenic property is desirable for the fabrication of vascular grafts or conduits. To this end, we have chemically modified chitosan by phthalization to derive an anti-thrombogenic material for the fabrication of vascular grafts. The chemical structure of phthalized chitosan was characterized with infrared spectroscopy. The hydrophilicity was examined with contact angle measurement, and the molecular weight distribution was measured using gel permeation chromatography (GPC). Protein adsorption, hemolysis, and platelet adhesion assays were used to confirm the enhanced anti-thrombogenic properties of this phthalized chitosan. Cytotoxicity and proliferation assays were performed to test its high biocompatibility. With its improved solubility and processibility, this phthalized chitosan was fabricated into selective permeable tubular constructs of varying sizes and morphology through a wet phase-inversion process. With improved anti-thrombogenic property, biocompatibility, and great processibility, phthalized chitosan has great potential as the material for the fabrication of small diameter vascular grafts.

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A simple instrument to characterize convective and diffusive transport of single hollow fibers of short length

Cited by 10 publications

References 24 publications

Development of a Perfusion Platform for Dynamic Cultivation of in vitro Skin Models

Development of a Perfusion Platform for Dynamic Cultivation of in vitro Skin Models

Development and evaluation of elastomeric hollow fiber membranes as small diameter vascular graft substitutes

Fabrication of permeable tubular constructs from chemically modified chitosan with enhanced antithrombogenic property

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