Development and evaluation of elastomeric hollow fiber membranes as small diameter vascular graft substitutes

Mercado-Pagán, Àngel E.; Kang, Yunqing; Findlay, Michael W.; Yang, Yunzhi Peter

doi:10.1016/j.msec.2015.01.051

Cited by 22 publications

(23 citation statements)

References 60 publications

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“…By day 9 high cell viability is demonstrated in all scaffolds, with the highest fraction of live cells present in scaffold S4 attributed to the provision of good cell access to nutrients throughout the whole scaffold. In contrast, much lower SMC cell viability has been reported for polyether urethane hollow fiber vascular grafts associated with reported low initial cell attachment to the synthetic scaffold.…”

Section: Resultsmentioning

confidence: 83%

Smooth muscle tissue engineering in crosslinked electrospun gelatin scaffolds

Elsayed

Lekakou

Labeed

et al. 2015

J Biomedical Materials Res

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Crosslinked, multi-layer electrospun gelatin fiber scaffolds with generally ±45 degree fiber orientation have been used to grow human umbilical vein smooth muscle cells (HUVSMCs) to create a vascular tunica media graft. Scaffolds of different fiber diameter (2-5 μm in wet state), pore size, and porosity (16-21% in wet state) were assessed in terms of cell adherence and viability, cell proliferation, and migration in both in-plane and transverse directions through the scaffold as a function of time under static cell culture conditions. HUVSMC cell viability reached between 80 and 92% for all scaffolds after 9 days in culture. HUVSMCs adhered, elongated, and orientated in the fiber direction, and migrated through a scaffold thickness of 200-235 μm 9 days post-seeding under static conditions. The best scaffold was then used to assess the tissue engineering of HUVSMCs under dynamic conditions for a rotating, cell seeded, tubular scaffold in the bioreactor containing the culture medium. Dynamic conditions almost doubled the rate of cell proliferation through the scaffold, forming full tissue throughout a scaffold of 250-300 μm thickness 6 days post-seeding.

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Section: Resultsmentioning

confidence: 83%

Smooth muscle tissue engineering in crosslinked electrospun gelatin scaffolds

Elsayed

Lekakou

Labeed

et al. 2015

J Biomedical Materials Res

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“…Obviously, appropriate size of micropores is needed for better endothelialization of ASDBVs. The micropore structure of ASDBVs could be realized by some methods, for example, salt leaching , coagulation , electrospinning , and phase‐inversion . Although these techniques could construct the micropores, the uncontrollability of the structure (for the former two) and the complicated procedures (for the latter two) always trouble the researchers in the field.…”

Section: Introductionmentioning

confidence: 99%

Small diameter blood vessels with controllable micropore structure induced by centrifugal force for improved endothelialization

Gao

Chen

et al. 2020

Engineering in Life Sciences

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The micropore structure is prerequisite for fast and durable endothelialization of artificial small diameter blood vessels (ASDBVs). Although some methods, such as salt leaching, coagulation, and electrospinning, have been developed to construct micropores for ASDBVs, the uncontrollability of the structure and the complicated procedures of the process are still the issues to be concerned about. In this study, a compact device based on the principle of centrifugal force is established and used to prepare polyurethane (PU) ASDBVs with micropore structures by blasting different porogens. It is found that the glass beads could construct micropores with regular round shape, uniform distribution, and controllable size (60-350 µm), which significantly improves the endothelialization of PU-based ASDBVs, especially when the pore size is about 60 µm. This method is easy-accessible and wide-applicable, which provides a new pathway for the research and development of ASDBVs. K E Y W O R D Scentrifugal force, endothelialization, micropore structure, polyurethane, small diameter blood vessel

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“…The presence of PEO increased porosity and elasticity in all the systems. Many reports have suggested the need for improve scaffold porosity and permeability to optimize tissue ingrowth for vascular grafts, 7,17,51,52 and these porous silk based microtubes should be able to address this need. With 13% silk in the blends higher porosity and pores of 20 μm average diameter (in the size range for better ingrowth tissue rates in humans) were found accompanied by higher cell attachment and degradability, parameters that can improve ingrowth as well.…”

Section: Discussionmentioning

confidence: 99%

Biodegradable porous silk microtubes for tissue vascularization

et al. 2017

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Cardiovascular diseases are the leading cause of mortality around the globe, and microvasculature replacements to help stem these diseases are not available. Additionally, some vascular surgeries needing small diameter vascular grafts present different performance requirements. In this work silk fibroin scaffolds based on silk/polyethylene oxide blends were developed as microtubes for vasculature needs and for different tissue regeneration times, mechanical properties and structural designs. Systems with 13, 14 and 15% silk alone or blended with 1 or 2% of polyethylene oxide (PEO) were used to generate porous microtubes using gel-spinning. Microtubes with inner diameters (ID) of 150–300 μm and 100 μm wall thickness were fabricated. The systems were assessed for porosity, mechanical properties, enzymatic degradability, and in vitro vascular endothelial cell attachment and metabolic activity. After 14 days all tubes supported the proliferation of cells and cell attachment increased with porosity. The silk tubes with PEO had similar crystallinity but higher elastic modulus compared with the systems without PEO. The silk (13%)/PEO (1%) system showed the highest porosity (20 μm pore diameters on average), highest cell attachment and fastest degradation profile. There was a good correlation between these parameters with silk concentration and the presence of PEO. The results demonstrate the ability to generate versatile and tunable tubular biomaterials based on silk-PEO-blends with potential for microvascular grafts.

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Development and evaluation of elastomeric hollow fiber membranes as small diameter vascular graft substitutes

Cited by 22 publications

References 60 publications

Smooth muscle tissue engineering in crosslinked electrospun gelatin scaffolds

Smooth muscle tissue engineering in crosslinked electrospun gelatin scaffolds

Small diameter blood vessels with controllable micropore structure induced by centrifugal force for improved endothelialization

Biodegradable porous silk microtubes for tissue vascularization

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