A simple interpolation formula for the rate of approach of particles or cells with tension-controlled shapes at arbitrary separations

Dimitrov, Dimiter S.; Stoicheva, Natalia G.; STEFANOVA, D

doi:10.1016/s0021-9797(84)80036-3

Cited by 4 publications

(2 citation statements)

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“…The cell face was split into a lower and upper portion to determine antibody flux to the portion of the cell where the CNA receptors responsible for firm adhesion are located. The split was made at a y distance of 0.03 m from the attachment base to ensure that the mass transfer calculation incorporated a greater distance than a typical nonstressed protein-protein interaction of 20 nm (Dimitrov et al, 1984). This split results in the lower portion of the sphere being approximately 3% of the total cell surface area.…”

Section: Computational Fluid Dynamicsmentioning

confidence: 99%

Fluid shear contributions to bacteria cell detachment initiated by a monoclonal antibody

Mascari

Ymele-Leki

Eggleton

et al. 2003

Biotech & Bioengineering

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Receptor-mediated adhesion of bacteria to biological surfaces is a significant step leading to infection. Due to an increase in bacterial antibiotic resistance, novel methods to block and disrupt these specific interactions have gained considerable interest as possible therapeutic strategies. Recently, several monoclonal antibodies specific for the Staphylococcus aureus collagen receptor demonstrated specialized ability to displace attached cells from collagen in static assays. In this study, we experimentally examine the monoclonal antibody detachment functionality under physiological shear conditions to evaluate the role of this parameter in the detachment process. The detachment of staphylococci from collagen was quantified in real-time using a parallel plate flow chamber, phase contrast video-microscopy and digital image processing. The results demonstrate a unimodal dependence of detachment on fluid wall shear rate. The observed decrease in effective detachment rate with increasing force at the highest shear levels evaluated is counterintuitive and has not been previously demonstrated. Several possible mechanisms of this result are discussed.

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Section: Computational Fluid Dynamicsmentioning

confidence: 99%

Fluid shear contributions to bacteria cell detachment initiated by a monoclonal antibody

Mascari

Ymele-Leki

Eggleton

et al. 2003

Biotech & Bioengineering

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“…The contact area radius was calculated as described in Kuo and Lauffenburger (1993) assuming a protein-protein interaction distance of ≈20 nm (Dimitrov et al, 1984), which yields a value of a c ‫ס‬ 0.0995 m. N/N CRIT is calculated by dividing the values given in Table I by the critical receptor number obtained from Eq. (A2-1).…”

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confidence: 99%

Novel experimental study of receptor-mediated bacterial adhesion under the influence of fluid shear

Mohamed

Rainier

Ross

2000

Biotechnol. Bioeng.

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Dynamic adhesion of cells to surfaces is a vital step in a variety of biochemical and physiological phenomena. Bacterial adhesion is responsible not only for problems associated with biofouling and biofilm formation in the biochemical industry but also in the initiation of certain infectious diseases. In this study, we report the effect of critical parameters, such as receptor and ligand densities and shear rate, on receptor‐mediated dynamic bacterial adhesion. Adhesion of a pathogenic strain of Staphylococcus aureus to immobilized collagen was studied. The receptor density on the cell surface was varied by harvesting cells at different growth times and was quantified using flow cytometry. Dynamic adhesion experiments were conducted over a range of physiologically relevant shear rates (50 to 1500 s−1) using a parallel‐plate flow chamber. Video microscopy coupled with digital image processing was employed to quantify adhesion. A semiquantitative comparison between experimental results and theoretical data obtained using a previously proposed mathematical model was also performed. The results suggest that dynamic adhesion is dependent on receptor density and shear rate, but independent of ligand density. This report demonstrates the feasibility of using bacteria to study fundamental aspects of receptor‐mediated dynamic adhesion. © 2000 John Wiley & Sons, Inc. Biotechnol Bioeng 68: 628–636, 2000.

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Dielectrophoresis, instability, and electrofusion in membrane systems

Dimitrov

Doinov

Trends in Colloid and Interface Science III

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A simple interpolation formula for the rate of approach of particles or cells with tension-controlled shapes at arbitrary separations

Cited by 4 publications

References 0 publications

Fluid shear contributions to bacteria cell detachment initiated by a monoclonal antibody

Fluid shear contributions to bacteria cell detachment initiated by a monoclonal antibody

Novel experimental study of receptor-mediated bacterial adhesion under the influence of fluid shear

Dielectrophoresis, instability, and electrofusion in membrane systems

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