Fluid shear contributions to bacteria cell detachment initiated by a monoclonal antibody

Mascari, Lisa; Ymele-Leki, Patrick; Eggleton, Charles D.; Speziale, Pietro; Ross, Julia M.

doi:10.1002/bit.10650

Cited by 25 publications

(30 citation statements)

References 35 publications

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“…In cases where cell adhesion to surfaces and host tissues occurs through the formation of catch bonds, our data disprove the common expectation that bacterial detachment rates, and thus the efficacy of soluble inhibitors, increases with the shear stress level (20). One possible advantage of catch bond-mediated surface interactions is that E. coli might take advantage of shear stressenhanced adhesion to evade detachment from body surfaces by soluble glycoproteins or peptides naturally found in our bodies.…”

Section: Discussionsupporting

confidence: 70%

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Elevated Shear Stress Protects Escherichia coli Cells Adhering to Surfaces via Catch Bonds from Detachment by Soluble Inhibitors

Nilsson

Thomas²,

Sokurenko

et al. 2006

Appl Environ Microbiol

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Soluble inhibitors find widespread applications as therapeutic drugs to reduce the ability of eukaryotic cells, bacteria, or viruses to adhere to surfaces and host tissues. Mechanical forces resulting from fluid flow are often present under in vivo conditions, and it is commonly presumed that fluid flow will further add to the inhibitive effect seen under static conditions. In striking contrast, we discover that when surface adhesion is mediated by catch bonds, whose bond life increases with increased applied force, shear stress may dramatically increase the ability of bacteria to withstand detachment by soluble competitive inhibitors. This shear stress-induced protection against inhibitor-mediated detachment is shown here for the fimbrial FimH-mannose-mediated surface adhesion of Escherichia coli. Shear stress-enhanced reduction of bacterial detachment has major physiological and therapeutic implications and needs to be considered when developing and screening drugs.

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Section: Discussionsupporting

confidence: 70%

“…Shear stress generally enhances the efficacy of soluble inhibitors (20). This might be expected considering the effect of drag force, since tensile forces acting on receptor-ligand complexes are thought to exponentially decrease the lifetime of slip bonds (6).…”

mentioning

confidence: 98%

Elevated Shear Stress Protects Escherichia coli Cells Adhering to Surfaces via Catch Bonds from Detachment by Soluble Inhibitors

Nilsson

Thomas²,

Sokurenko

et al. 2006

Appl Environ Microbiol

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“…Particular focus has been given to the viscoelasticity of cell attachment and detachment, under the influence of antibiotics and flows with different shear rates [14][15][16]. In these studies, the analysis of cells detachment was performed only in small time slots, at specific biofilm growth stages.…”

Section: Introductionmentioning

confidence: 99%

Living bacteria rheology: Population growth, aggregation patterns, and collective behavior under different shear flows

et al. 2014

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The activity of growing living bacteria was investigated using real-time and in situ rheologyin stationary and oscillatory shear. Two different strains of the human pathogen Staphylococcus aureus -strain COL and its isogenic cell wall autolysis mutant -were considered in this work. For low bacteria density, strain COL forms small clusters, while the mutant, presenting deficient cell separation, forms irregular larger aggregates. In the early stages of growth, when subjected to a stationary shear, the viscosity of both strains increases with the population of cells. As the bacteria reach the exponential phase of growth, the viscosity of the two strains follow different and rich behaviours, with no counterpart in the optical density or in the population's colony forming units measurements. While the viscosity of strain COL keeps increasing during the exponential phase and returns close to its initial value for the late phase of growth, where the population stabilizes, the viscosity of the mutant strain decreases steeply, still in the exponential phase, remains constant for some time and increases again, reaching a constant plateau at a maximum value for the late phase of growth. These complex viscoelastic behaviours, which were observed to be shear stress dependent, are a consequence of two coupled effects: the cell density continuous increase and its changing interacting properties. The viscous and elastic moduli of strain COL, obtained with oscillatory shear, exhibit power-law behaviours whose exponent are dependent on the bacteria growth stage. The viscous and elastic moduli of the mutant have complex behaviours, emerging from the different relaxation times that are associated with the large molecules of the medium and the self-organized structures of bacteria. These behaviours reflect nevertheless the bacteria growth stage.

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“…1,2 Such infections are a growing concern because of the increasing antibiotic resistance of S aureus. 1,3 Specifically, vancomycin resistance (the antibiotic of "last resort") was first reported in 1996. 4 A recent fact sheet (2003) by the Centre for Disease Control (CDC) has reported a total of 10 separate cases in the United States, thereby suggesting increasing vancomycin resistance.…”

mentioning

confidence: 99%

“…However, previous studies investigating S aureus-platelet binding mechanisms have been performed under static conditions and do not account for a potential shear force effect. 1,22 In order to closely mimic the in vivo environment in vitro, we use collagen type I to support deposition of a platelet layer. This methodology allows for the study of S aureus adhesion mechanisms to immobilized platelets under shear stress conditions relevant to the vasculature.…”

mentioning

confidence: 99%

Staphylococcus aureus Adhesion via Spa, ClfA, and SdrCDE to Immobilized Platelets Demonstrates Shear-Dependent Behavior

George

Shin

et al. 2006

ATVB

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Objective-The objective of this study is to delineate the molecular mechanisms responsible for Staphylococcus aureus-platelet adhesion as a function of physiologically relevant wall shear stresses. Methods and Results-A parallel plate flow chamber was used to quantify adhesion of wild-type, Spa Ϫ , ClfA Ϫ and SdrCDE Ϫ strains to immobilized platelet layers. In the absence of plasma, adhesion increases with increasing wall shear rate from 100 to 5000 seconds Ϫ1 . The presence of plasma significantly enhances adhesion at all shear levels. Addition of exogenous fibrinogen yields adhesion levels similar to plasma in the lower shear regimes, but has a diminishing effect on potentiating adhesion at higher shear rates. Alternatively, as shear rate increases von Willebrand factor (VWF) plays an increasingly significant role in mediating binding. Conclusions-Addition of plasma proteins potentiates S aureus-platelet

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Fluid shear contributions to bacteria cell detachment initiated by a monoclonal antibody

Cited by 25 publications

References 35 publications

Elevated Shear Stress Protects Escherichia coli Cells Adhering to Surfaces via Catch Bonds from Detachment by Soluble Inhibitors

Elevated Shear Stress Protects Escherichia coli Cells Adhering to Surfaces via Catch Bonds from Detachment by Soluble Inhibitors

Living bacteria rheology: Population growth, aggregation patterns, and collective behavior under different shear flows

Staphylococcus aureus Adhesion via Spa, ClfA, and SdrCDE to Immobilized Platelets Demonstrates Shear-Dependent Behavior

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