<i>Staphylococcus aureus</i>
            Adhesion via Spa, ClfA, and SdrCDE to Immobilized Platelets Demonstrates Shear-Dependent Behavior

George, Niraj Procopio Evagrio; Qi, Wei; Shin, Pilsoo; Κωνσταντόπουλος, Κωνσταντίνος; Ross, Julia M.

doi:10.1161/01.atv.0000237606.90253.94

Cited by 49 publications

(18 citation statements)

References 39 publications

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“…The strain was isolated from a patient diagnosed with osteomyelitis and has been extensively studied [9,10,33]. S. aureus cultures were started by inoculation (10 μL) from glycerol stocks into tryptic soy broth (TSB; 50 μL) supplemented with 0.25% (w/v) glucose and grown at 37°C with constant rotation at 141 rpm in shake flasks.…”

Section: Methodsmentioning

confidence: 99%

Proteomic analysis of Staphylococcus aureus biofilm cells grown under physiologically relevant fluid shear stress conditions

et al. 2014

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BackgroundThe biofilm forming bacterium Staphylococcus aureus is responsible for maladies ranging from severe skin infection to major diseases such as bacteremia, endocarditis and osteomyelitis. A flow displacement system was used to grow S. aureus biofilms in four physiologically relevant fluid shear rates (50, 100, 500 and 1000 s-1) to identify proteins that are associated with biofilm.ResultsGlobal protein expressions from the membrane and cytosolic fractions of S. aureus biofilm cells grown under the above shear rate conditions are reported. Sixteen proteins in the membrane-enriched fraction and eight proteins in the cytosolic fraction showed significantly altered expression (p < 0.05) under increasing fluid shear. These 24 proteins were identified using nano-LC-ESI-MS/MS. They were found to be associated with various metabolic functions such as glycolysis / TCA pathways, protein synthesis and stress tolerance. Increased fluid shear stress did not influence the expression of two important surface binding proteins: fibronectin-binding and collagen-binding proteins.ConclusionsThe reported data suggest that while the general metabolic function of the sessile bacteria is minimal under high fluid shear stress conditions, they seem to retain the binding capacity to initiate new infections.

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Section: Methodsmentioning

confidence: 99%

Proteomic analysis of Staphylococcus aureus biofilm cells grown under physiologically relevant fluid shear stress conditions

et al. 2014

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“…ClfA and ClfB are covalently attached to the cell wall and mediate bacterial adherence to immobilized Fg, blood clots, conditioned biomaterial ex vivo, and thrombindamaged heart valves in a rat model of endocarditis. The proteins also promote bacterial clumping in the presence of soluble Fg [5,[7][8][9][10][11][12][13][14]. In addition, several secreted proteins of S. aureus can bind to Fg, notably coagulase, the bifunctional fibronectin-binding proteins A and B (FnbpA and FnbpB), the extracellular Fg-binding protein (Efb), and MHC class II analogue protein (Map) [7,[15][16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Rot and Agr system modulate fibrinogen-binding ability mainly by regulating clfB expression in Staphylococcus aureus NCTC8325

Xue

You

Shang

et al. 2011

Med Microbiol Immunol

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Staphylococcus aureus is an important human pathogen that causes a variety of diseases, ranging from localized skin infections to life-threatening systemic infections. The success of S. aureus as a pathogen is partly due to its ability to adhere to a wide range of host tissues by binding to host extracellular matrix proteins such as fibrinogen, fibronectin, and collagen. Staphylococcus aureus expresses two proteins that can bind specifically to fibrinogen, clumping factors A and B (ClfA and ClfB). Repressor of toxins (Rot) is known to be a global regulator of virulence gene expression in S. aureus. The translation of Rot is regulated by the staphylococcal accessory gene regulator (Agr) quorum-sensing system. In this study, we demonstrated that Rot and the Agr system in S. aureus NCTC8325 can affect the bacterial binding ability to human fibrinogen (Fg) under different bacterial growth phases. Our real-time RT-PCR results indicated that both Rot and the Agr system have no significant effect on clfA expression. However, Rot is an activator of clfB, and Agr/RNAIII can regulate clfB expression via Rot. Gel shift data further suggested that Rot might regulate clfB expression by directly binding to the promoter region of clfB. Moreover, Rot and the Agr system exhibited consistent regulatory effects on clfB transcription and bacterial Fg-binding ability, suggesting that Rot and the Agr system might affect bacterial Fg-binding ability mainly through regulating clfB transcription.

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“…Excess liposomes were removed, and platelets were stained with a fluorescent antibody against CD42b, a cell-surface marker expressed only on platelets and megakaryocytes. [16] Liposomes co-localized with 35 to 65 % of platelets, with variation between platelet donors (Figure 2a). Confocal microscopy of liposome-treated platelets confirmed that the majority of co-localization corresponded to internalized liposomes (Figure 2b).…”

Section: Resultsmentioning

confidence: 99%

Controlled Transcription of Exogenous mRNA in Platelets Using Protocells

et al. 2015

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Transcribing exogenous RNA in eukaryotic cells requires delivering DNA to their nuclei and changing their genome. Nuclear delivery is often inefficient, limiting the potential scope of gene therapy and synthetic biology. These challenges may be overcome by techniques that allow for extranucleate transcription within eukaryotic cells. Protocells have been developed that enable transcription inside of liposomes; however, it has not yet been demonstrated whether this technology can be extended for use within eukaryotic cells. Here we show RNA-synthesizing nanoliposomes allow transcription of exogenous RNA inside anucleate cells. To accomplish this, components of transcription were encapsulated into liposomes and delivered to platelets. These liposomes were capable of light-induced transcription in platelets, providing proof-of-concept that protocell technology can be adapted for use within mammalian cells.

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Staphylococcus aureus Adhesion via Spa, ClfA, and SdrCDE to Immobilized Platelets Demonstrates Shear-Dependent Behavior

Cited by 49 publications

References 39 publications

Proteomic analysis of Staphylococcus aureus biofilm cells grown under physiologically relevant fluid shear stress conditions

Proteomic analysis of Staphylococcus aureus biofilm cells grown under physiologically relevant fluid shear stress conditions

Rot and Agr system modulate fibrinogen-binding ability mainly by regulating clfB expression in Staphylococcus aureus NCTC8325

Controlled Transcription of Exogenous mRNA in Platelets Using Protocells

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