2014
DOI: 10.1270/jsbbs.64.156
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A simple method based on laboratory inoculum and field inoculum for evaluating potato resistance to black scurf caused by <i>Rhizoctonia solani</i>

Abstract: A two-step method was developed to evaluate potato resistance to black scurf caused by Rhizoctonia solani. Tuber piece inoculum was first conducted in the laboratory, which was also first reported in this study. After inoculation with pathogen discs and culture for 48 h, the necrotic spots on the inoculated potato pieces were generated and measured by the crossing method. Further evaluation was conducted through field experiments using a wheat bran inoculum method. The wheat bran inoculum was placed into the p… Show more

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Cited by 29 publications
(20 citation statements)
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“…The chilling injury of the fruits during storage was qualitatively determined based on visual observation using a numerical rating scale of 1-4 (1 = no chilling injury, 2 = slight chilling injury, 3 = moderate chilling injury, 4 = severe chilling injury). The disease incidence was calculated (Zhang et al 2014…”
Section: Moisture Contentmentioning
confidence: 99%
“…The chilling injury of the fruits during storage was qualitatively determined based on visual observation using a numerical rating scale of 1-4 (1 = no chilling injury, 2 = slight chilling injury, 3 = moderate chilling injury, 4 = severe chilling injury). The disease incidence was calculated (Zhang et al 2014…”
Section: Moisture Contentmentioning
confidence: 99%
“…Festuca arundinacea) were placed on the soil surface, covered with 1 cm of sterile natural soil, and watered with 9-10 ml of water. The infected plants were counted 3 weeks later (Zhang et al 2014) and recorded. Evaluation was made according to a scale of 0 to 5: 0 = no disease symptoms, 1 = 1-10% hypocotyl infecting and/or shortening, 2 = 11-30% hypocotyl infecting and/or shortening, 3 = 31-50% hypocotyl infecting and/or shortening, 4 = 51-80% hypocotyl infecting and/or shortening, and 5 = entire hypocotyl infecting and/or shortening (Ichielevich Auster et al 1985).…”
Section: Pathogenicity Testsmentioning
confidence: 99%
“…According to Zhang et al [16] with some modification, healthy potato tubers selected and washed carefully in water. Tubers dipped in ethanol 70% for 5 min and washed in distilled water.…”
Section: Pathogenicity Testmentioning
confidence: 99%