A Simple Method with Liquid Chromatography/Tandem Mass Spectrometry for the Determination of the Six Trichothecene Mycotoxins in Rice Medium

Sagawa, Norifumi; Takino, Takashi; Kurogochi, Shin

doi:10.1271/bbb.70.230

Cited by 19 publications

(9 citation statements)

References 14 publications

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“…For example, in one study a selective and speedy LC-MS/MS method was developed to determine six trichothecene mycotoxins in rice medium. The analytes were extracted from the rice medium and diluted with acetonitrile/water (85/15, v/v) in order to minimize the effects of matrices [102]. Diluted solutions were analyzed by LC-MS/MS with electrospray ionization (ESI) interface in negative or positive ion mode and the multiple reaction monitoring mode.…”

Section: Sample Dilutionmentioning

confidence: 99%

Compensate for or Minimize Matrix Effects? Strategies for Overcoming Matrix Effects in Liquid Chromatography-Mass Spectrometry Technique: A Tutorial Review

et al. 2020

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In recent decades, mass spectrometry techniques, particularly when combined with separation methods such as high-performance liquid chromatography, have become increasingly important in pharmaceutical, bio-analytical, environmental, and food science applications because they afford high selectivity and sensitivity. However, mass spectrometry has limitations due to the matrix effects (ME), which can be particularly marked in complex mixes, when the analyte co-elutes together with other molecules, altering analysis results quantitatively. This may be detrimental during method validation, negatively affecting reproducibility, linearity, selectivity, accuracy, and sensitivity. Starting from literature and own experience, this review intends to provide a simple guideline for selecting the best operative conditions to overcome matrix effects in LC-MS techniques, to obtain the best result in the shortest time. The proposed methodology can be of benefit in different sectors, such as pharmaceutical, bio-analytical, environmental, and food sciences. Depending on the required sensitivity, analysts may minimize or compensate for ME. When sensitivity is crucial, analysis must try to minimize ME by adjusting MS parameters, chromatographic conditions, or optimizing clean-up. On the contrary, to compensate for ME analysts should have recourse to calibration approaches depending on the availability of blank matrix. When blank matrices are available, calibration can occur through isotope labeled internal standards and matrix matched calibration standards; conversely, when blank matrices are not available, calibration can be performed through isotope labeled internal standards, background subtraction, or surrogate matrices. In any case, an adjusting of MS parameters, chromatographic conditions, or a clean-up are necessary.

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Section: Sample Dilutionmentioning

confidence: 99%

Compensate for or Minimize Matrix Effects? Strategies for Overcoming Matrix Effects in Liquid Chromatography-Mass Spectrometry Technique: A Tutorial Review

et al. 2020

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“…To analyse each of these mycotoxins individually is expensive and time-consuming. Even with advanced analytical instruments such as LC-MS/MS (Sagawa et al 2006;Cavaliere et al 2007), it is impossible to analyse for all of the hundreds of known mycotoxins. If one could detect fungal growth generally, it would be very useful.…”

Section: Please Scroll Down For Articlementioning

confidence: 99%

Single-laboratory validation of a method for ergosterol determination in cereals

Miyagawa

Umeda

Sato

et al. 2009

Food Additives & Contaminants: Part A

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Ergosterol (ERG) is a sterol produced by most fungi, but not by most plants. Thus, measurement of ERG in cereals makes it possible to determine the presence of fungi in cereals that can cause quality problems, such as mycotoxin contamination. This study developed and performed a single-laboratory validation for a method to test for ERG in various cereals. ERG was extracted by refluxing samples for 1 h with methanol-sodium hydroxide. ERG was extracted from the extract with hexane and then purified using a silica gel cartridge column. ERG was then separated and detected by reverse-phase high-performance liquid chromatography (HPLC). 'Within-day' recoveries of ERG at low levels were 92-99% with relative standard deviations (RSDs) of 3.2-6.5%. 'Between-day' recoveries of ERG at low levels were 97% and RSDs were 4.2-10.2%, respectively. Average recoveries of ERG over the range from 1.0 to 100.0 mg kg(-1) were 81-105% and RSDs were 3.9-16.3%.

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“…In recent years, the availability of ionization sources, i.e. APCI and ESI, has significantly improved the possibilities of employing LC/MS in the mycotoxin analysis, due to their high sensitivity, simple sample preparation and their compatibility with almost the whole range of compound polarities 3, 10, 29–34. No other technique in the area of instrumental analysis has developed so rapidly as LC‐MS/MS during the past 10 years 35, 36.…”

Section: Introductionmentioning

confidence: 99%

A rapid method with ultra‐high‐performance liquid chromatography–tandem mass spectrometry for simultaneous determination of five type B trichothecenes in traditional Chinese medicines

Han

Liu

Ren

et al. 2010

J of Separation Science

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A speedy and selective ultra-HPLC-MS/MS method for simultaneous determination of deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-ADON), 15-ADON, nivalenol and fusarenon X in traditional Chinese medicines (TCMs) was developed. The method was based on one-step sample cleanup using reliable homemade cleanup cartridges. A linear gradient mobile-phase system, consisting of water containing 0.2% aqueous ammonia and acetonitrile/methanol (90:10, v/v) at a flow rate of 0.4 mL/min, and an Acquity UPLC HSS T3 column (100 mm x 2.1 mm, 1.8 microm) were employed to obtain the best resolution of the target analytes. [(13)C(15)]-DON was used as the internal standard to accomplish as accurate as possible quantitation. The established method was further validated by determining the linearity (R(2) > or = 0.9990), sensitivity (LOQ, 0.29-0.99 microg/kg), recovery (88.5-119.5%) and precision (RSD < or = 15.8%). It was shown to be a suitable method for simultaneous determination of DON, 3-ADON, 15-ADON, nivalenol and fusarenon X in various TCM matrices. The utility and practical impact of the method was demonstrated using different TCM samples.

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A Simple Method with Liquid Chromatography/Tandem Mass Spectrometry for the Determination of the Six Trichothecene Mycotoxins in Rice Medium

Cited by 19 publications

References 14 publications

Compensate for or Minimize Matrix Effects? Strategies for Overcoming Matrix Effects in Liquid Chromatography-Mass Spectrometry Technique: A Tutorial Review

Compensate for or Minimize Matrix Effects? Strategies for Overcoming Matrix Effects in Liquid Chromatography-Mass Spectrometry Technique: A Tutorial Review

Single-laboratory validation of a method for ergosterol determination in cereals

A rapid method with ultra‐high‐performance liquid chromatography–tandem mass spectrometry for simultaneous determination of five type B trichothecenes in traditional Chinese medicines

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