A simple methodology for predicting the performances of hyaluronic acid purification by diafiltration

Oueslati, Nadia; Leblanc, Pierrick; Bodin, Alice; Harscoat–Schiavo, Christelle; Rondags, Emmanuel; Meunier, Stéphane; Marc, Ivan; Kapel, Romain

doi:10.1016/j.memsci.2015.04.024

Cited by 15 publications

(6 citation statements)

References 17 publications

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“…Samples were collected after each dia‐volume for analysis. During the course of diafiltration process parameters such as retention and purity were calculated . Membrane washing and storage was done using 0.1 N sodium hydroxide.…”

Section: Methodsmentioning

confidence: 99%

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Design of aqueous two‐phase systems for purification of hyaluronic acid produced by metabolically engineered Lactococcus lactis

Rajendran

Puvendran

Guru

et al. 2016

J of Separation Science

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Hyaluronic acid has a wide range of biomedical applications and its commercial value is highly dependent on its purity and molecular weight. This study highlights the utility of aqueous two-phase separation as a primary recovery step for hyaluronic acid and for removal of major protein impurities from fermentation broths. Metabolically engineered cultures of a lactate dehydrogenase mutant strain of Lactococcus lactis (L. lactis NZ9020) were used to produce high-molecular-weight hyaluronic acid. The cell-free fermentation broth was partially purified using a polyethylene glycol/potassium phosphate system, resulting in nearly 100% recovery of hyaluronic acid in the salt-rich bottom phase in all the aqueous two-phase separation experiments. These experiments were optimized for maximum removal of protein impurities in the polyethylene glycol rich top phase. The removal of protein impurities resulted in substantial reduction of membrane fouling in the subsequent diafiltration process, carried out with a 300 kDa polyether sulfone membrane. This step resulted in considerable purification of hyaluronic acid, without any loss in recovery and molecular weight. Diafiltration was followed by an adsorption step to remove minor impurities and achieve nearly 100% purity. The final hyaluronic acid product was characterized by Fourier-transform IR and NMR spectroscopy, confirming its purity.

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Section: Methodsmentioning

confidence: 99%

“…and Zhou et al. have exclusively used diafiltration technique for HA purification, with 90% recovery and purity. Our investigations with ultrafiltration (UF) as the initial HA purification step showed that the membranes are susceptible to fouling due to the high amount of protein impurities from the fermentation broth.…”

Section: Introductionmentioning

confidence: 99%

Design of aqueous two‐phase systems for purification of hyaluronic acid produced by metabolically engineered Lactococcus lactis

Rajendran

Puvendran

Guru

et al. 2016

J of Separation Science

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“…This behavior was explained by a reduction in the intra‐ and intermolecular electrostatic interactions under these conditions, although no information was provided on the relative contribution of these interactions. Oueslati et al used a diafiltration process to remove small peptides and proteins in the purification of hyaluronic acid. The filtrate flux actually increased during the diafiltration process, which the authors attributed to the reduction in solution ionic strength and its effects on both the mass transfer coefficient and the wall concentration.…”

Section: Introductionmentioning

confidence: 99%

Effect of electrostatic interactions on the ultrafiltration behavior of charged bacterial capsular polysaccharides

Hadidi

Buckley

Zydney

2016

Biotechnology Progress

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Charged polysaccharides are used in the food industry, as cosmetics, and as vaccines. The viscosity, thermodynamics, and hydrodynamic properties of these charged polysaccharides are known to be strongly dependent on the solution ionic strength because of both inter- and intramolecular electrostatic interactions. The goal of this work was to quantitatively describe the effect of these electrostatic interactions on the ultrafiltration behavior of several charged capsular polysaccharides obtained from Streptococcus pneumoniae and used in the production of Pneumococcus vaccines. Ultrafiltration data were obtained using various Biomax™ polyethersulfone membranes with different nominal molecular weight cutoffs. Polysaccharide transmission decreased with decreasing ionic strength primarily because of the expansion of the charged polysaccharide associated with intramolecular electrostatic repulsion. Data were in good agreement with a simple theoretical model based on solute partitioning in porous membranes, with the effective size of the different polysaccharide serotypes evaluated using size exclusion chromatography at the same ionic conditions. These results provide fundamental insights and practical guidelines for exploiting the effects of electrostatic interactions during the ultrafiltration of charged polysaccharides. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1531-1538, 2016.

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“…This technology has been used for the recovery of specific target molecules, like dextran, from complex fermentation systems [ 47 ]. As an example, Oueslati et al [ 28 ] also worked with Streptococcus zooepidemicus to produce HA of more than 90% purity with a molecular weight of 1.5 × 10 6 Da. The extraction was done by DF with a planar polyethersulfone (PES) membrane with a transmembrane pressure of 2.5 bar at RT.…”

Section: Advances In Ha Bacterial Production Extraction and Purificationmentioning

confidence: 99%

A Review on Current Strategies for Extraction and Purification of Hyaluronic Acid

Rodriguez-Marquez

Arteaga-Marin

Rivas-Sánchez

et al. 2022

IJMS

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Since it is known that hyaluronic acid contributes to soft tissue growth, elasticity, and scar reduction, different strategies of producing HA have been explored in order to satisfy the current demand of HA in pharmaceutical products and formulations. The current interest deals with production via bacterial and yeast fermentation and extraction from animal sources; however, the main challenge is the right extraction technique and strategy since the original sources (e.g., fermentation broth) represent a complex system containing a number of components and solutes, which complicates the achievement of high extraction rates and purity. This review sheds light on the main pathways for the production of HA, advantages, and disadvantages, along with the current efforts in extracting and purifying this high-added-value molecule from different sources. Particular emphasis has been placed on specific case studies attempting production and successful recovery. For such works, full details are given together with their relevant outcomes.

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A simple methodology for predicting the performances of hyaluronic acid purification by diafiltration

Cited by 15 publications

References 17 publications

Design of aqueous two‐phase systems for purification of hyaluronic acid produced by metabolically engineered Lactococcus lactis

Design of aqueous two‐phase systems for purification of hyaluronic acid produced by metabolically engineered Lactococcus lactis

Effect of electrostatic interactions on the ultrafiltration behavior of charged bacterial capsular polysaccharides

A Review on Current Strategies for Extraction and Purification of Hyaluronic Acid

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