A Simple Parallel Analytical Method of Prenatal Screening

Li, Ding; Yang, Hao; Zhang, Wenhong; Pan, Hao; Wen, Dongqing; Han, Fengchan; Guo, Haipeng; Wang, Xiaoming; Yan, Xiaojun

doi:10.1159/000094092

Cited by 6 publications

(4 citation statements)

References 38 publications

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“…About 15 nm monodisperse colloidal gold particles were prepared according to the method described before by the authors [22]. Briefly, 50 mL (0.2 mg/mL) chloroauric acid was heated to boiling point, and then 1.2 mL (10 mg/mL) sodium citrate was added.…”

Section: Methodsmentioning

confidence: 99%

A Novel Quantum Dots–Based Point of Care Test for Syphilis

Yang

Ding²,

et al. 2010

Nanoscale Res Lett

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One-step lateral flow test is recommended as the first line screening of syphilis for primary healthcare settings in developing countries. However, it generally shows low sensitivity. We describe here the development of a novel fluorescent POC (Point Of Care) test method to be used for screening for syphilis. The method was designed to combine the rapidness of lateral flow test and sensitiveness of fluorescent method. 50 syphilis-positive specimens and 50 healthy specimens conformed by Treponema pallidum particle agglutination (TPPA) were tested with Quantum Dot-labeled and colloidal gold-labeled lateral flow test strips, respectively. The results showed that both sensitivity and specificity of the quantum dots–based method reached up to 100% (95% confidence interval [CI], 91–100%), while those of the colloidal gold-based method were 82% (95% CI, 68–91%) and 100% (95% CI, 91–100%), respectively. In addition, the naked-eye detection limit of quantum dot–based method could achieve 2 ng/ml of anti-TP47 polyclonal antibodies purified by affinity chromatography with TP47 antigen, which was tenfold higher than that of colloidal gold–based method. In conclusion, the quantum dots were found to be suitable for labels of lateral flow test strip. Its ease of use, sensitiveness and low cost make it well-suited for population-based on-the-site syphilis screening.

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Section: Methodsmentioning

confidence: 99%

A Novel Quantum Dots–Based Point of Care Test for Syphilis

Yang

Ding²,

et al. 2010

Nanoscale Res Lett

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“…Typically, the TORCH is referred as a classical group of five teratogenic pathogens including Toxoplasmosis (TOX), Rubella virus (RUB), Cytomegalovirus (CMV), and Herpes simplex virus type 1 and 2 (HSV-1, HSV-2) (Jong et al 2013). TORCH screening to the pregnant women is helpful to provide critical clinical information for antenatal care (Li et al 2006). Different methods, for example, ELISA (enzyme linked immunosorbent assay), CLIA (chemiluminescence), ECLIA (electrochemiluminiscence) based on IgG or IgM have been developed for TORCH screening (Ursu et al 2015).…”

Section: Introductionmentioning

confidence: 99%

A bead-based microfluidic system for joint detection in TORCH screening at point-of-care testing

Qiu

Zhang

et al. 2017

Microsyst Technol

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A concise bead-based microfluidic system has been developed for joint detection in TORCH screening at point-of-care testing. Assisted by five functionalized polycarbonate single beads, totally five critical infections including TOX, RUB, CMV, HSV-1 and HSV-2 can be simultaneously detected with the microfluidic chip based on chemiluminescence. The microfluidic chip consists of multiple reaction chambers respectively for different reaction steps of the sandwiched chemiluminescence immunoassay, and each reaction chamber is divided into five interconnected independent cells to respectively hold five single beads in different position. With a guiding path, each single bead with a ferrous core can be easily transported between each two adjacent reaction chambers through magnetic control to consecutively react with the serum sample or other different reagents. Meanwhile, with magnetic control, each single bead within the reaction chamber can be moved back and forth to improve the detection sensitivity with enhanced mixing. In one time test, joint detection with five infections to one serum sample can be simultaneously implemented by introducing synchronous manipulation to five single beads. Chemiluminescence signal from five single beads is collected by a sensitive CCD camera in parallel. Qualitative detection is achieved by comparing the detection signal amplitude with the corresponding cutoff value for each infection. Experimental results show that accurate, simple, convenient and efficient TORCH screening can be achieved with the easyto-operate and low-cost microfluidic system at resourcepoor settings.

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“…If ToRCH-related pathogens infect pregnant women, they will cause severe fetal anomalies or even fetal loss [21,22]. For this reason, it is of great practical significance to establish a simple, sensitive detection method for ToRCH pathogens.…”

Section: Introductionmentioning

confidence: 99%

A Quick and Parallel Analytical Method Based on Quantum Dots Labeling for ToRCH-Related Antibodies

Yang

Guo

et al. 2009

Nanoscale Res Lett

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Quantum dot is a special kind of nanomaterial composed of periodic groups of II–VI, III–V or IV–VI materials. Their high quantum yield, broad absorption with narrow photoluminescence spectra and high resistance to photobleaching, make them become a promising labeling substance in biological analysis. Here, we report a quick and parallel analytical method based on quantum dots for ToRCH-related antibodies including Toxoplasma gondii, Rubella virus, Cytomegalovirus and Herpes simplex virus type 1 (HSV1) and 2 (HSV2). Firstly, we fabricated the microarrays with the five kinds of ToRCH-related antigens and used CdTe quantum dots to label secondary antibody and then analyzed 100 specimens of randomly selected clinical sera from obstetric outpatients. The currently prevalent enzyme-linked immunosorbent assay (ELISA) kits were considered as “golden standard” for comparison. The results show that the quantum dots labeling-based ToRCH microarrays have comparable sensitivity and specificity with ELISA. Besides, the microarrays hold distinct advantages over ELISA test format in detection time, cost, operation and signal stability. Validated by the clinical assay, our quantum dots-based ToRCH microarrays have great potential in the detection of ToRCH-related pathogens.

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A Simple Parallel Analytical Method of Prenatal Screening

Cited by 6 publications

References 38 publications

A Novel Quantum Dots–Based Point of Care Test for Syphilis

A Novel Quantum Dots–Based Point of Care Test for Syphilis

A bead-based microfluidic system for joint detection in TORCH screening at point-of-care testing

A Quick and Parallel Analytical Method Based on Quantum Dots Labeling for ToRCH-Related Antibodies

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