A Simple, Rapid Method for Determination of Melatonin in Plant Tissues by UPLC Coupled with High Resolution Orbitrap Mass Spectrometry

Ye, Tiantian; Yan, Hao; Yu, Lei; Shi, Haitao; Reíter, Russel J.; Feng, Yu‐Qi

doi:10.3389/fpls.2017.00064

Cited by 33 publications

(23 citation statements)

References 47 publications

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“…Based on the presence of melatonin metabolic enzymes and alternate pathways with relatively low kinetics of melatonin biosynthetic enzymes in plants compared with that in animals, there is no reason for plants to produce high quantities of melatonin relative to animals. In accordance with our speculations as mentioned above, several recent publications have revealed that plants produce melatonin at concentrations far lower than nanogram per gram weight in healthy tissues during their normal growth and development …”

Section: Discussionsupporting

confidence: 93%

Melatonin induction and its role in high light stress tolerance in Arabidopsis thaliana

Lee

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2018

Journal of Pineal Research

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In plants, melatonin is a potent bioactive molecule involved in the response against various biotic and abiotic stresses. However, little is known of its defensive role against high light (HL) stress. In this study, we found that melatonin was transiently induced in response to HL stress in Arabidopsis thaliana with a simultaneous increase in the expression of melatonin biosynthetic genes, including serotonin N-acetyltransferase1 (SNAT1). Transient induction of melatonin was also observed in the flu mutant, a singlet oxygen ( O )-producing mutant, upon light exposure, suggestive of melatonin induction by chloroplastidic O against HL stress. An Arabidopsis snat1 mutant was devoid of melatonin induction upon HL stress, resulting in high susceptibility to HL stress. Exogenous melatonin treatment mitigated damage caused by HL stress in the snat1 mutant by reducing O production and increasing the expression of various ROS-responsive genes. In analogy, an Arabidopsis SNAT1-overexpressing line showed increased tolerance of HL stress concomitant with a reduction in malondialdehyde and ion leakage. A complementation line expressing an Arabidopsis SNAT1 genomic fragment in the snat1 mutant completely restored HL stress susceptibility in the snat1 mutant to levels comparable to that of wild-type Col-0 plants. The results of the analysis of several Arabidopsis genetic lines reveal for the first time at the genetic level that melatonin is involved in conferring HL stress tolerance in plants.

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Section: Discussionsupporting

confidence: 93%

Melatonin induction and its role in high light stress tolerance in Arabidopsis thaliana

Lee

Back

2018

Journal of Pineal Research

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“…). Although the melatonin concentration of 100 µ M is beyond its physiological range in plants (Ye et al ), the reason for its powerful effect may be that melatonin is oxidized easily under light (Hardeland ), and the available concentration of melatonin was not entirely because of spraying. Therefore, further studies should consider soil applications of melatonin.…”

Section: Discussionmentioning

confidence: 99%

Hydrogen peroxide produced by NADPH oxidase: a novel downstream signaling pathway in melatonin‐induced stress tolerance in Solanum lycopersicum

Gong

Yan

Wen

et al. 2017

Physiologia Plantarum

142

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The beneficial effects of melatonin on abiotic stress have been demonstrated in several plants. However, little is known about the signal transduction pathway of melatonin involved in the plant stress response. Here, we manipulated the melatonin levels in tomato plants through a chemical approach. The roles of melatonin in stress tolerance were studied by assessing the symptoms, chlorophyll fluorescence and stress-responsive gene expression. Moreover, both chemical and genetic approaches were used to study the roles of hydrogen peroxide (H O ) in melatonin-induced signal transduction in tomato plants. We found that melatonin activates NADPH oxidase (RBOH) to enhance H O levels by reducing its S-nitrosylation activity. Furthermore, melatonin-induced H O accumulation was accompanied by obtainable stress tolerance. Inhibition of RBOH or chemical scavenging of H O significantly reduced the melatonin-induced defense response, including reduced expression of several stress-related genes (CDPK1, MAPK1, TSPMS, ERF4, HSP80 and ERD15) and reduced antioxidative enzyme activity (SOD, CAT and APX), which were responsible for the stress tolerance. Collectively, these results revealed a novel mechanism in which RBOH activity and H O signaling are important components of the melatonin-induced stress tolerance in tomato plants.

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“…Melatonin (MT) was extracted following the procedure described by Ye et al with minor modifications. Leaf samples (1 g fresh weight) were snap‐frozen in liquid nitrogen, then powdered and extracted in 10 mL acetonitrile.…”

Section: Methodsmentioning

confidence: 99%

Strigolactone represses the synthesis of melatonin, thereby inducing floral transition in Arabidopsis thaliana in an FLC‐dependent manner

Zhang

Liu

et al. 2019

Journal of Pineal Research

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The transition from vegetative to reproductive growth is a key developmental event in a plant's life cycle. The process is mediated by a combination of phytohormones, including melatonin (MT) and strigolactone (SL). Here, the Arabidopsis mutants, d14-1 and max4-1, which are compromised with respect to either SL synthesis or signaling, were shown to flower earlier than wild types. The tissue MT content in both mutants was higher than in wild types, as a result of the up-regulation of various genes encoding enzymes involved in MT synthesis. The abundance in the mutants of transcripts derived from each of the genes SPLs, AP1, and SOC1 was reduced with exogenously supplied MT, while FLC was induced. Plants exposed to a high concentration of MT did not flower earlier than wild types. The tissue MT content of a mutant unable to synthesize caffeic acid O-methyltransferase was less than that of wild type and flowered earlier than did wild types. The suggestion is that the flowering time of Arabidopsis is altered if the tissue content of MT is either higher than ~ 8 ng/g F.W, or lower than ~ 0.9 ng/g. Within this range, SL acts to determine flowering time by its regulation of SPL genes. The application of exogenous SL reduces tissue MT content. The flowering time of the flc-3 mutant was unaffected by exogenously supplying either MT or/and SL. It is proposed that MT acts downstream of SL to activate FLC, inducing a delay to flowering if its concentration lies outside a certain range. K E Y W O R D SFLC, floral transition, SPLs, strigolactone mutants, ultra-high-performance liquid chromatography

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A Simple, Rapid Method for Determination of Melatonin in Plant Tissues by UPLC Coupled with High Resolution Orbitrap Mass Spectrometry

Cited by 33 publications

References 47 publications

Melatonin induction and its role in high light stress tolerance in Arabidopsis thaliana

Melatonin induction and its role in high light stress tolerance in Arabidopsis thaliana

Hydrogen peroxide produced by NADPH oxidase: a novel downstream signaling pathway in melatonin‐induced stress tolerance in Solanum lycopersicum

Strigolactone represses the synthesis of melatonin, thereby inducing floral transition in Arabidopsis thaliana in an FLC‐dependent manner

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