A simple, rapid method for detecting seven common invasive fish species in Europe from environmental DNA

Clusa, Laura; García‐Vázquez, Eva

doi:10.1002/aqc.2890

Cited by 19 publications

(15 citation statements)

References 90 publications

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“…Moreover, as this is a non‐lethal method, it would be suitable for monitoring populations of endangered anguillid eels in the same way as it has been applied to other endangered species, including fishes (Boothroyd, Mandrak, Fox, & Wilson, ; Eva et al, ; Laramie, Pilliod, & Goldberg, ; Pfleger, Rider, Johnston, & Janosik, ), bivalves (Currier, Morris, Wilson, & Freeland, ), and amphibians (Fukumoto et al, ). This method could also be used to monitor species of invasive eels, as it has been used for other invasive species (Clusa & García‐Vázquez, ; Dougherty et al, ; Hinlo, Furlan, Suitor, & Gleeson, ). Non‐native eel species (e.g.…”

Section: Discussionmentioning

confidence: 99%

Environmental DNA analysis reveals the spatial distribution, abundance, and biomass of Japanese eels at the river‐basin scale

Itakura

Wakiya

Yamamoto

et al. 2019

Aquatic Conservation

106

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1. There is growing international concern about declines in populations of anguillid eels, resulting in their inclusion in the International Union for the Conservation of Nature (IUCN) Red List of Threatened Species. Monitoring the population dynamics of these species is often challenging, however, owing to their broad distributions and complex, catadromous life histories.2. Whether environmental DNA (eDNA) analysis could be used to monitor the spatial distribution of anguillid eels in rivers was investigated by conducting basin-scale surveys of Japanese eels, Anguilla japonica Temminck & Schlegel, 1846, in 10 rivers in Japan, and comparing the results obtained using eDNA analysis with the results obtained using the electrofishing method. Moreover, the relationship between the eDNA concentration and the abundance and biomass of Japanese eels was examined.3. The eDNA of Japanese eels was detected at 56 (91.8%) of the 61 study sites from which individuals were collected by electrofishing, and at an additional 35 sites where individuals were not directly collected. This indicates that eDNA analysis has a greater sensitivity for detecting the presence of eels, making it a powerful tool for monitoring the spatial distribution of anguillid eels in rivers. 4. A significant, but weak, positive relationship between the eDNA concentration and the abundance and biomass of Japanese eels was also found, suggesting that eDNA analysis may be useful for estimating the abundance and biomass of anguillid eels in rivers. 5. This is the first study to demonstrate the potential usefulness of eDNA analysis for estimating the spatial distribution, abundance, and biomass of Japanese eels in rivers. eDNA analysis will allow anguillid eel populations to be monitored over large spatial and temporal scales using a consistent protocol, with reduced time and effort compared with conventional techniques, providing invaluable information for managing populations of these endangered species.

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Section: Discussionmentioning

confidence: 99%

Environmental DNA analysis reveals the spatial distribution, abundance, and biomass of Japanese eels at the river‐basin scale

Itakura

Wakiya

Yamamoto

et al. 2019

Aquatic Conservation

106

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“…Here, sampling was performed at the shore of the river and the lake around 1-m depth. The sampling strategy was the same as that used in Ebro River (Clusa and Garcia-Vazquez 2018), which, like the Rhine River, is a big river with high flow and rapid current speed. Indeed, it is possible that the DNA of some species, especially for those fish swimming far from the shore, was at very low concentration and remained undetected.…”

Section: Discussionmentioning

confidence: 99%

Nuisance species in lake constance revealed through eDNA

et al. 2021

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Biological invasions are a global threat to biodiversity especially for aquatic resources. The distribution of alien species is associated with human activities; therefore, exotic species tend to accumulate near big urban areas through different invasion vectors such as ballast water, hull fouling, aquarium and pet releases. The Rhine River region is one of the most economically important in Europe. Around 60 million people live in the river basin that is connected with other large European rivers via the Rhine-Main-Danube shipping canal. The Alpine Rhine flows to Lake Constance, which is the second largest subalpine lake in Europe. Here, eDNA metabarcoding was employed to inventory aquatic species from water samples in six riverine and four lake localities within Lake Constance region. A 313 bp fragment within cytochrome c oxidase subunit I gene was PCR amplified using generalist primers for metazoan and sequenced with MiSeq High-Throughput Sequencing platform. Seven invertebrate invasive species and the invasive fish Oncorhynchus mykiss were detected from eDNA. Species-specific primers were employed to confirm metabarcoded species. Most of the invasive species detected in this study correspond to samples from areas around lake ports, followed by other lake and degraded downstream river areas. Samples taken upstream of Lake Constance were free of invertebrate aliens. To establish common regulation and management actions regarding aquatic invasions in the three countries that share Lake Constance is recommended.

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“…It is often difficult to pinpoint the exact location of an invasion front because individuals at the invasion front are present at low-density and are therefore difficult to detect. In recent years environmental DNA (eDNA) has started to be used as an AIS monitoring tool due to its high sensitivity, non-invasive sampling, and cost effectiveness (Herder et al, 2014;Clusa and García-Vázquez, 2018;Geerts et al, 2018). The method is based on the detection of species from extracellular DNA, or cell debris, that species leave behind in the environment (Valentini et al, 2009).…”

Section: Important Considerations In the Application Of Ais Exclusion Barriersmentioning

confidence: 99%

The Use of Barriers to Limit the Spread of Aquatic Invasive Animal Species: A Global Review

et al. 2021

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Aquatic invasive species (AIS) are one of the principal threats to freshwater biodiversity. Exclusion barriers are increasingly being used as a management strategy to control the spread of AIS. However, exclusion barriers can also impact native organisms and their effectiveness is likely to be context dependent. We conducted a quantitative literature review to evaluate the use of barriers to control animal AIS in freshwater ecosystems worldwide. The quantitative aspect of the review was supplemented by case studies that describe some of the challenges, successes, and opportunities for the use of the use of AIS exclusion barriers globally. Barriers have been used since the 1950s to control the spread of AIS, but effort has been increasing since 2005 (80% of studies) and an increasingly diverse range of AIS taxa are now targeted in a wide range of habitat types. The global use of AIS barriers has been concentrated in North America (74% of studies), Australasia (11%), and Europe (10%). Physical barriers (e.g., weirs, exclusion screens, and velocity barriers) have been most widely used (47%), followed by electric (27%) and chemical barriers (12%). Fish were the most targeted taxa (86%), followed by crustaceans (10%), molluscs (3%) and amphibians (1%). Most studies have been moderately successful in limiting the passage of AIS, with 86% of the barriers tested deterring >70% of individuals. However, only 25% of studies evaluated barrier impacts on native species, and development of selective passage is still in its infancy. Most studies have been too short (47% < 1 year, 87% < 5 years) to detect ecological impacts or have failed to use robust before-after-control-impact (BACI) study designs (only 5%). Hence, more effective monitoring is required to assess the long-term effectiveness of exclusion barriers as an AIS management tool. Our global case studies highlight the pressing need for AIS control in many ecoregions, and exclusion barriers have the potential to become an effective tool in some situations. However, the design and operation of exclusion barriers must be refined to deliver selective passage of native fauna, and exclusion barriers should only be used sparingly as part of a wider integrated management strategy.

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A simple, rapid method for detecting seven common invasive fish species in Europe from environmental DNA

Cited by 19 publications

References 90 publications

Environmental DNA analysis reveals the spatial distribution, abundance, and biomass of Japanese eels at the river‐basin scale

Environmental DNA analysis reveals the spatial distribution, abundance, and biomass of Japanese eels at the river‐basin scale

Nuisance species in lake constance revealed through eDNA

The Use of Barriers to Limit the Spread of Aquatic Invasive Animal Species: A Global Review

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