A Simple Sample Preparation with HPLC - UV Method for Estimation of Amlodipine from Plasma: Application to Bioequivalence Study

Meyyanathan, S. N.; Muralidharan, Selvadurai; Rajan, Srinivasan; Gopal, K.; Suresh, B.

doi:10.2174/1875038900801010022

Cited by 13 publications

(7 citation statements)

References 5 publications

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“…C max and T max after oral administration were calculated directly from the plasma concentration-time curve using the WinNonlin ™ Nonlinear Estimation Program. One-way analysis of variance (ANOVA) was employed to assess the significance of the difference between the tested ALS-SLN formulation and the reference at a level of p≤ 0.05 using the SPSS program [ 21 ]. AUC 0-24 was calculated using the linear trapezoidal rule [ 22 ].…”

Section: Methodsmentioning

confidence: 99%

Alendronate Sodium as Enteric Coated Solid Lipid Nanoparticles; Preparation, Optimization, and In Vivo Evaluation to Enhance Its Oral Bioavailability

Hosny

2016

PLoS ONE

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Treatment of osteoporosis with alendronate sodium has several challenges. The first challenge is the low bioavailability. The second main challenge is side effects, which include oesophageal ulceration. The aim of this research was to reformulate alendronate sodium as enteric coated solid lipid nanoparticles in order to enhance its bioavailability, and preventing the free alendronate sodium from coming into direct contact with the gastrointestinal mucosa, and thereby reducing the possibility of side effects. Enteric coated solid lipid nanoparticles were prepared according to the Box-Behnken design employing Design expert® software, and characterized for size, morphology, and entrapment efficiency. The optimized formula was coated with an Eudragit S100 and evaluated for drug release in acidic and basic media, stability studies and pharmacokinetic evaluations on rabbits. The results indicated that, using Derringer's desirability functional tool for optimization, the highest entrapment efficiency value of 74.3% and the smallest size value of 98 nm were predicted under optimum conditions with a desirability value of 0.917. The optimized nanoparticles released alendronate sodium only at an alkaline pH. The pharmacokinetic evaluation revealed that alendronate sodium bioavailability was enhanced by more than 7.4-fold in rabbits. In conclusion, enteric coated solid lipid nanoparticles is a promising formula for the delivery of alendronate sodium, eliminating its oesophageal side effects and enhancing its bioavailability.

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Section: Methodsmentioning

confidence: 99%

Alendronate Sodium as Enteric Coated Solid Lipid Nanoparticles; Preparation, Optimization, and In Vivo Evaluation to Enhance Its Oral Bioavailability

Hosny

2016

PLoS ONE

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“…One-way analysis of variance (ANOVA) was employed to assess the significance of the difference between the T max , C max , and AUC 0–∞ data of ferrous sulfate obtained from the control tablet and Fe-SLN formulations ( P ≤0.05) using the SPSS program. 31 The AUC 0–24 was calculated using the linear trapezoidal rule. The AUC 0–∞ and he relative bioavailability (BA R ) of the SLNs were also calculated by using the following equation 32 :

…”

Section: Methodsmentioning

confidence: 99%

Solid lipid nanoparticles loaded with iron to overcome barriers for treatment of iron deficiency anemia

Hosny¹,

Banjar²,

Hariri³

et al. 2015

DDDT

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According to the World Health Organization, 46% of the world's children suffer from anemia, which is usually treated with iron supplements such as ferrous sulfate. The aim of this study was to prepare iron as solid lipid nanoparticles, in order to find an innovative way for alleviating the disadvantages associated with commercially available tablets. These limitations include adverse effects on the digestive system resulting in constipation and blood in the stool. The second drawback is the high variability in the absorption of iron and thus in its bioavailability. Iron solid lipid nanoparticles (Fe-SLNs) were prepared by hot homogenization/ultrasonication. Solubility of ferrous sulfate in different solid lipids was measured, and effects of process variables such as the surfactant type and concentration, homogenization and ultrasonication times, and charge-inducing agent on the particle size, zeta potential, and encapsulation efficiency were determined. Furthermore, in vitro drug release and in vivo pharmacokinetics were studied in rabbits. Results indicated that Fe-SLNs consisted of 3% Compritol 888 ATO, 1% Lecithin, 3% Poloxamer 188, and 0.2% dicetylphosphate, with an average particle size of 25 nm with 92.3% entrapment efficiency. In vivo pharmacokinetic study revealed more than fourfold enhanced bioavailability. In conclusion, Fe-SLNs could be a promising carrier for iron with enhanced oral bioavailability.

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“…We decided on Aml as the IS in this study. Aml is a well-known molecule, being stable, easy to measure, and mentioned in many manuscripts in the literature, − suggesting that it is a usable molecule.…”

Section: Resultsmentioning

confidence: 99%

Determination of Tryptophan and Kynurenine by LC–MS/MS by Using Amlodipine as an Internal Standard

Eser

Özkan

Dinçel

2020

J. Am. Soc. Mass Spectrom.

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Tryptophan is an essential amino acid that plays an important role in cell metabolism, and kynurenine is its main metabolic pathway. By using ultra-high-performance liquid chromatography coupled to electrospray ionization triple–quadrupole mass spectrometry, tryptophan and kynurenine were determined using amlodipine as an internal standard. The analysis was carried out on an ACE-C18 (4.6 mm × 50 mm, 5 μm) reversed-phase analytical column using the gradient elution mode. For quantitative determination, amlodipine was used as an internal standard. Detection was performed using multiple reaction monitoring in electrospray ionization mode at m/z 205.1 → 117.7 and 187.9 for tryptophan, m/z 209.1 → 146 and 93.9 for kynurenine, and m/z 409.2 → 294.1 for the internal standard. Good linearity of the analyte to internal standard peak area ratios was seen in the concentration range 1.25–4000 ng/mL for tryptophan and 0.5–1600 ng/mL for kynurenine. The method showed excellent linearity with regression coefficients of 0.99 for kynurenine and 0.996 for tryptophan. The limits of quantification were 0.55 ng/mL for tryptophan and 0.47 ng/mL for kynurenine. The % RSD for all analytes ranged from 0.3 to 3.4% for intraday and 0.4 to 8.9% for interday experiments. A simple LC–MS/MS method has been developed and validated for measuring Kyn and Trp by using an affordable and more easily available internal standard, which is amlodipine.

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A Simple Sample Preparation with HPLC - UV Method for Estimation of Amlodipine from Plasma: Application to Bioequivalence Study

Cited by 13 publications

References 5 publications

Alendronate Sodium as Enteric Coated Solid Lipid Nanoparticles; Preparation, Optimization, and In Vivo Evaluation to Enhance Its Oral Bioavailability

Alendronate Sodium as Enteric Coated Solid Lipid Nanoparticles; Preparation, Optimization, and In Vivo Evaluation to Enhance Its Oral Bioavailability

Solid lipid nanoparticles loaded with iron to overcome barriers for treatment of iron deficiency anemia

Determination of Tryptophan and Kynurenine by LC–MS/MS by Using Amlodipine as an Internal Standard

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