A Simple, Selective and Sensitive Immunoassay for Determination of Human Chorionic Gonadotrophin Based on Chemiluminescence Resonance Energy Transfer

Abstract

A simple, selective and sensitive immunoassay was developed for the determination of human chorionic gonadotrophin (HCG) based on the chemiluminescence resonance energy transfer (CRET). Two kinds of antibodies were used to form a sandwich-type immunocomplex to ensure the selectivity of immunoassay. Based on the simple magnetic separation, amplification feature of gold nanoparticles and CRET phenomenon, this method could be used for highly sensitive determination of HCG without tedious washing steps. Under the optimized conditions, a linear range was obtained when the concentrations of HCG were changed from 0.15 to 5 mIU mL -1 (R = 0.992). This immunoassay could be used for the determination of HCG in serum samples, which provided a promising potential in clinical diagnosis.

Electrochemical measurement of antibody-antigen recognition biophysics: Thermodynamics and kinetics of human chorionic gonadotropin (hCG) binding to redox-tagged antibodies

“…Since from equation ( 7), e A θ corresponds to the ratio of the Frumkin binding constant (K, lateral interactions exist) to the Langmurian binding constant (K lang , no lateral interactions exist), using equation (18), the approximation made is…”

“…The majority of hCG immunoassays most commonly use enzyme--linked immunosorbent assay (ELISA) and other sandwich assay techniques [17], with recent advancements reported using chemiluminescence [18,19], fluorescence [20], and electrochemical immunosensor approaches [21--23], in addition to on-disc advancements [24]. The diagnostic cut--off for a positive reading is generally 25 milliinternational units per millilitre (mIU mL --1 ), corresponding to 2.7 ng mL --1 , although this varies significantly from test to test [25].…”

A facile and sensitive peptide-modulating graphene oxide nanoribbon catalytic nanoplasmon analytical platform for human chorionic gonadotropin

“…We can see that the RRS analytical system is more sensitive than the Abs and was thus chosen for sample detection. Compared to the reported hCG methods, [25][26][27][28][29][30][31][32][33][34][35][36] this RRS method is not only sensitive and selective but also facile, of low-cost and without nanoparticle aggregation. The clinical method of chemiluminescent immunoassay had a DL of 0.143 ng/mL, limit of quantitation (LQ) of 5 ng/mL and linear range of 5-540 ng/mL hCG; 37 the DL and LQ of this RRS method are better than the clinical method, and the linear range of the two methods are close to each other, as the upper LQ divided by the lower LQ is 108 and 100, respectively.…”

“…At present, the detection methods of hCG are mainly electrochemical immunoassay, radioimmunoassay, chemiluminescent immunoassay, fluorescence resonance energy transfer method and optical immunoassay. [25][26][27][28][29][30] Among these methods, radioimmunoassay is widely used, but there are radiation hazards. The electrochemical immunoassay method has a high sensitivity, but the operation is complex.…”

A Novel Magnetism‐assisted Electrochemical Immunosensor with Sub‐Picomolar Sensitivity

“…In recent decades, electrochemical immunosensors have found a unique place in the determination of biological and environmental analytes due to their advantages of high sensitivity, low cost, as well as the ease of operation and miniaturization . At present, a major challenge is to develop these immunosensors with picomolar or better concentration sensitivity (i.e., limits of detection).…”