A Simple Spectrophotometric Method for the Estimation of .ALPHA.-Carotene, .BETA.-Carotene and Lycopene Concentrations in Carrot Acetone Extracts

雅靖, 永田; 裕司, 野口; 秀和, 伊藤; 俊介, 今西; 慶太, 杉山

doi:10.3136/nskkk.54.351

Cited by 19 publications

(9 citation statements)

References 10 publications

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“…After subtraction of the value of a blank, as obtained using 0.2 ml of distilled water as a sample, differences of absorbance were used for estimation of total GSL contents. According to the method used by Nagata (2009) and Nagata et al (2007) , the 136 samples were divided into two groups by choosing one alternately from the sample with the highest GSL content. One group was used as the calibration dataset.…”

Section: Methodsmentioning

confidence: 99%

Small variation of glucosinolate composition in Japanese cultivars of radish (<i>Raphanus sativus</i> L.) requires simple quantitative analysis for breeding of glucosinolate component

et al. 2012

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To reveal varietally differing glucosinolate (GSL) contents in radish (Raphanus sativus L.) cultivated in Japan, the total and individual GSLs of 28 cultivars were analyzed using high-performance liquid chromatography. In these cultivars, GSL types including three aliphatic GSLs (glucoraphenin, glucoerucin, and 4-methylthio-3-butenyl GSL (4MTB-GSL)) and three indolyl GSLs (4-hydroxyglucobrassicin, glucobrassicin, and 4-methoxy-glucobrassicin) were detected. No cultivar-specific type of GSL was identified. The dominant GSL was 4MTB-GSL, but its contents differed remarkably: 8.6 μmol/g in ‘Koushin’ to 135.7 μmol/g in ‘Karami 199’. Over about 90% of all GSLs in Japanese radish type are 4MTB-GSL, a higher percentage than in Chinese or European garden radish cultivars. A simple, rapid method for estimating total GSL contents in crude extracts was established because of the small variation of glucosinolate composition in Japanese cultivars. The total GSL content can be estimated using an equation for prediction with absorbance at 425 nm in a mixture of GSL crude extract and palladium (II) chloride solution: Total GSL (μmol/g) = 305.47 × A425 − 29.66. Its coefficient of determination (R2) and standard error of prediction (SEP) are 0.968 and 8.052. This method enables total GSL content estimation from more than 200 samples per person per day.

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Section: Methodsmentioning

confidence: 99%

Small variation of glucosinolate composition in Japanese cultivars of radish (<i>Raphanus sativus</i> L.) requires simple quantitative analysis for breeding of glucosinolate component

et al. 2012

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“…Standard procedure with little modification was followed for estimation of β-carotene contents (Nagata, 2009). Polished rice grains were grounded using a pestle and mortar.…”

Section: Estimation Of β-Carotenementioning

confidence: 99%

GENETIC DIVERSITY IN TRADITIONAL GENOTYPES FOR GRAIN IRON, ZINC AND Β-Carotene CONTENTS REVEAL POTENTIAL FOR BREEDING MICRONUTRIENT DENSE RICE

Raza¹,

Saher²,

Shahzadi³

et al. 2019

JEBAS

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Rice is the staple food for half of the world"s population, however, its edible grain part is deficient in essential micronutrients, especially Fe and Zn. Breeding for micronutrient dense rice demands exploration of available genetic diversity for grain Fe, Zn and β-carotene contents. In this study, we analysed brown and polished grain samples of 26 traditional rice genotypes for Fe and Zn concentration. Fe concentration varied from 13.23 ppm to 45.83 ppm and 1.10 ppm to 36.45 ppm in brown and polished rice, respectively. Whereas, Zn concentration in brown and polished rice ranged from 18.67 ppm to 38.01 ppm and 5.86 ppm to 23.88 ppm, respectively. The landraces had higher Fe and Zn contents than improved varieties and advance uniform lines. Significant positive correlation was present between Fe and Zn concentration in brown (r = 0.694, P < 0.01) and polished rice (r = 0.533, P < 0.01). Fe concentration was greatly reduced by the polishing process than Zn concentration and approved varieties lost less Fe and Zn contents than advance uniform lines. Minimal levels of beta-carotene were detected in rice genotypes indicating need for genetic modifications to enhance pro-vitamin A contents in rice endosperm.

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“…b-carotene content b-carotene content in each sample was determined using the method described by Nagata, Noguchi, Ito, Imanishi, and Sugiyama (2007). The carrot sample was weighed, homogenized with acetone, and the slurry filtered and centrifuged twice for 10 min at 12,500g.…”

Section: Ascorbic Acid Contentmentioning

confidence: 99%

Effects of mildly heated, slightly acidic electrolyzed water on the disinfection and physicochemical properties of sliced carrot

et al. 2011

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A Simple Spectrophotometric Method for the Estimation of .ALPHA.-Carotene, .BETA.-Carotene and Lycopene Concentrations in Carrot Acetone Extracts

Cited by 19 publications

References 10 publications

Small variation of glucosinolate composition in Japanese cultivars of radish (<i>Raphanus sativus</i> L.) requires simple quantitative analysis for breeding of glucosinolate component

Small variation of glucosinolate composition in Japanese cultivars of radish (<i>Raphanus sativus</i> L.) requires simple quantitative analysis for breeding of glucosinolate component

GENETIC DIVERSITY IN TRADITIONAL GENOTYPES FOR GRAIN IRON, ZINC AND Β-Carotene CONTENTS REVEAL POTENTIAL FOR BREEDING MICRONUTRIENT DENSE RICE

Effects of mildly heated, slightly acidic electrolyzed water on the disinfection and physicochemical properties of sliced carrot

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