A simple technique for blood collection in the pig

Hu, Caihong; Cheang, A.; Retnam, L.; Yap, Edward

doi:10.1258/002367793780745606

Cited by 8 publications

(4 citation statements)

References 3 publications

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“…4 Swine were sedated prior to sampling, and a 19-to 21-gauge needle was used. A technique has been described 5 for IV catheter implantation in the subcutaneous abdominal vein in adult domestic swine. Results of the study indicated that there are few permanent sequelae to subcutaneous abdominal vein catheterization.…”

Section: Discussionmentioning

confidence: 99%

Use of the subcutaneous abdominal vein for blood sampling and intravenous catheterization in potbellied pigs

Snook¹

2001

javma

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The subcutaneous abdominal vein was used successfully to deliver fluids IV after catheterization and as a means of blood collection in 6 potbellied pigs of various body sizes and types. Because a 16-gauge catheter could be easily introduced into the vein, a large volume of fluid could be administered rapidly. Most pigs were highly tolerant of blood collection from this vein, and little restraint was required. The catheters remained in place for as long as 1 week, and no serious sequelae resulted from catheterization of the subcutaneous abdominal vein.

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Section: Discussionmentioning

confidence: 99%

Use of the subcutaneous abdominal vein for blood sampling and intravenous catheterization in potbellied pigs

Snook¹

2001

javma

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“…All animals were surgically implanted with vascular-access catheters to permit collection of serial blood samples and for medication administration (e.g., analgesics, antibiotics if necessary) postoperatively. Porcine animal models pose a special challenge for long-term vascular access given their size and lack of physical restraint [ 22 ]-[ 24 ]. Catheters were initially placed in the external jugular vein percutaneously, but early problems with this technique led us to switch to tunneled placement in the internal jugular vein under anesthesia.…”

Section: Methodsmentioning

confidence: 99%

A Large Animal Survival Model to Evaluate Bariatric Surgery Mechanisms

Simianu¹,

Sham²,

Wright³

et al. 2015

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BackgroundThe impact of Roux-en-Y gastric bypass (RYGB) on type 2 diabetes mellitus is thought to result from upper and/or lower gut hormone alterations. Evidence supporting these mechanisms is incomplete, in part because of limitations in relevant bariatric-surgery animal models, specifically the lack of naturally insulin-resistant large animals. With overfeeding, Ossabaw swine develop a robust metabolic syndrome, and may be suitable for studying post-surgical physiology. Whether bariatric surgery is feasible in these animals with acceptable survival is unknown.MethodsThirty-two Ossabaws were fed a high-fat, high-cholesterol diet to induce obesity and insulin resistance. These animals were assigned to RYGB (n = 8), RYGB with vagotomy (RYGB-V, n = 5), gastrojejunostomy (GJ, n = 10), GJ with duodenal exclusion (GJD, n = 7), or sham operation (n = 2) and were euthanized 60 days post-operatively. Post-operative changes in weight and food intake are reported.ResultsSurvival to scheduled necropsy among surgical groups was 77%, living an average of 57 days post-operatively. Cardiac arrest under anesthesia occurred in 4 pigs. Greatest weight loss (18.0% ± 6%) and food intake decrease (57.0% ± 20%) occurred following RYGB while animals undergoing RYGB-V showed only 6.6% ± 3% weight loss despite 50.8% ± 25% food intake decrease. GJ (12.7% ± 4%) and GJD (1.2% ± 1%) pigs gained weight, but less than sham controls (13.4% ± 10%).ConclusionsA survival model of metabolic surgical procedures is feasible, leads to significant weight loss, and provides the opportunity to evaluate new interventions and subtle variations in surgical technique (e.g. vagus nerve sparing) that may provide new mechanistic insights.

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“…Several techniques for placing intravenous catheters in pigs have been described in the scientific literature. [5][6][7][8][9][10][11][12] Although intravenous catheterization of 8-and 10-week-old pigs is routinely conducted in different research facilities, such catheterization in neonatal and younger pigs is challenging. Moreover, most research papers reporting the use of intravenous catheters in these age categories do not provide adequate details of the catheterization protocol, limiting the ability of other researchers to adopt these techniques.…”

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confidence: 99%

Repetitive urine and blood sampling in neonatal and weaned piglets for pharmacokinetic and pharmacodynamic modelling in drug discovery: a pilot study

et al. 2017

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Piglets are considered to be suitable animal models for predicting the pharmacokinetics and pharmacodynamics (PK/PD) of test drugs for potential use in the paediatric population. Such PK/PD studies require multiple blood and urine samplings. The goal of the present study was to determine a suitable blood collection strategy applicable in the youngest age categories of six days, four weeks and eight weeks of age, as well as a urine collection technique for male piglets in the same age categories. Blood was collected either by a surgically-placed jugular vein catheter (six days old [ n = 4] and four weeks old [ n = 2] piglets) or by direct venepuncture of the jugular vein (four weeks old [ n = 2] and eight weeks old [ n = 4] piglets). A non-invasive method for total volume urine collection in male piglets was also developed using a urine pouch. No specific complications were encountered during anaesthesia or surgery for jugular catheter placement. After a 24 h recovery period, urine and blood were easily collected without technical complications. One piglet was humanely killed at week 2 because of septicaemia. Histological analysis of both veins in all four piglets revealed negligible damage to the blood vessel wall. In conclusion, the presented techniques for blood (jugular catheter and direct venepuncture) and urine collection (pouches) are suitable for PK/PD studies in piglets.

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A simple technique for blood collection in the pig

Cited by 8 publications

References 3 publications

Use of the subcutaneous abdominal vein for blood sampling and intravenous catheterization in potbellied pigs

Use of the subcutaneous abdominal vein for blood sampling and intravenous catheterization in potbellied pigs

A Large Animal Survival Model to Evaluate Bariatric Surgery Mechanisms

Repetitive urine and blood sampling in neonatal and weaned piglets for pharmacokinetic and pharmacodynamic modelling in drug discovery: a pilot study

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