A simple technique for long‐term preservation of leptospires

Samir, Ahmed; Wasfy, Momtaz O.

doi:10.1002/jobm.201100551

Cited by 5 publications

(2 citation statements)

References 11 publications

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“…As the virulence of pathogenic Leptospira tends to attenuate during in vitro passages and freeze storage (Reed et al., 2000; Samir and Wasfy, 2013), the assessment of virulence requires a restoration step in hamsters by serial infections (Haake, 2006). For this, two serial passages in hamsters were performed and the resulting isolates were subsequently used for experimental infection.…”

Section: Methodsmentioning

confidence: 99%

Three Leptospira Strains From Western Indian Ocean Wildlife Show Highly Distinct Virulence Phenotypes Through Hamster Experimental Infection

Cordonin

Turpin

Bascands³

et al. 2019

Front. Microbiol.

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Leptospirosis is one of the most widespread zoonoses worldwide, with highest incidence reported on tropical islands. Recent investigations carried out in a One-Health framework have revealed a wide diversity of pathogenic Leptospira lineages on the different islands of Western Indian Ocean carried out by a large diversity of mammal reservoirs, including domestic and wild fauna. Using golden Syrian hamsters as a model of acute infection, we studied the virulence of Leptospira interrogans, L. mayottensis, and L. borgpetersenii isolates obtained from rats, tenrecs, and bats, respectively. Hamsters were inoculated with 2.108 bacterial cells and monitored for 1 month. The L. interrogans isolate proved to be the most pathogenic while L. mayottensis and L. borgpetersenii isolates induced no clinical symptoms in the infected hamsters. High leptospiral DNA amounts were also detected in the urine and organs of hamsters infected with the L. interrogans isolate while L. mayottensis and L. borgpetersenii isolates mostly failed to disseminate into the organism. In addition, histological damage was more pronounced in the kidneys and lungs of hamsters infected with the L. interrogans isolate. Altogether, these data support that Leptospira strains shed by mammals endemic to this insular ecosystem (L. mayottensis and L. borgpetersenii isolates) are less pathogenic than the L. interrogans rat-borne isolate. These results may provide a relevant framework for understanding the contrasting epidemiology of human leptospirosis observed among Western Indian Ocean islands.

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Section: Methodsmentioning

confidence: 99%

Three Leptospira Strains From Western Indian Ocean Wildlife Show Highly Distinct Virulence Phenotypes Through Hamster Experimental Infection

Cordonin

Turpin

Bascands³

et al. 2019

Front. Microbiol.

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“…T h e r e s e a r c h e r c l a s s i f i e d t h e Cryoprotectants as penetrating or non-penetrating the cell, Glycerol belongs to the latter one 3 . It is one of the most important types of cell-conserving substances 18 , that act to surround the cells, delay the freezing of intracellular substances in these cells and reduce the solution effects 1 . It prevents ice crystallization and as a result, bacteria remain alive longer 3 , it is a strong substance that prevents water from entering the cell 19 .…”

Section: Discussionmentioning

confidence: 99%

Comparison of Preservation Methods of Staphylococcus aureus and Escherichia coli Bacteria

Mahmmoud¹

2020

J Pure Appl Microbiol

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One of the most important problems faced by microbiologists is to preserve bacterial isolates in the best state to study and further diagnosis. The current study aims to provide a summary of experimental results to maintain two species of bacteria alive after being stored by using some additives. This study found that the best temperature to preserve Staphylococcus aureus was -20°C for a year, while for Escherichia coli it was the same temperature except in using Glycerol (G) 100% and Food oil (FO) methods. The optimum method to preserve S. aureus was by using Normal Saline (NS), while Distilled Water (DW) was the optimum method to preserve E. coli at temperatures (4, 25 and -20)°C for a year, the phenotypic patterns for examining bacteria were maintained except in NS at 4°C for S. aureus after a year ago. Glycerol was used alone at concentrations (100, 50, 30 and 15)%, and another group used G+NS in the same volumes, good results were achieved when it used alone or with NS to preserve bacteria for six months at 4°C except for methods of G100% and (G100% + NS) for examining bacteria. FO has never been used as preservation liquid, it is successful to survive S. aureus at -20°C for a year, and when it was added to NS, E. coli survived for a year at three temperatures (4, 25 and -20)°C, while S. aureus didn’t survive for a year when FO+NS method used at room temperature. The precipitation method was used for bacterial suspension, then added the preserving liquid, but the results were not effective compared to the First method.

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Leptospiral Structure, Physiology, and Metabolism

Cameron

2014

Current Topics in Microbiology and Immunology

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Members of the family Leptospiraceae are thin, spiral, highly motile bacteria that are best visualized by darkfield microscopy. These characteristics are shared with other members of the Order Spirochaetales, but few additional parallels exist among spirochetes. This chapter describes basal features of Leptospira Leptospira that are central to survival and, in the case of pathogenic leptospiral species, intimately linked with pathogenesis, including its morphology, characteristic motility, and unusual metabolism. This chapter also describes the general methodology and critical requirements for in vitro cultivation and storage of Leptospira within a laboratory setting.

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A simple technique for long‐term preservation of leptospires

Cited by 5 publications

References 11 publications

Three Leptospira Strains From Western Indian Ocean Wildlife Show Highly Distinct Virulence Phenotypes Through Hamster Experimental Infection

Three Leptospira Strains From Western Indian Ocean Wildlife Show Highly Distinct Virulence Phenotypes Through Hamster Experimental Infection

Comparison of Preservation Methods of Staphylococcus aureus and Escherichia coli Bacteria

Leptospiral Structure, Physiology, and Metabolism

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