A simple TLC and HPTLC method for separation of selected steroid drugs

Abstract

Abstract:© Versita Sp. z o.o. Keywords: Principal component analysis • Separation factor • Standard (Gibbs) free energy change of partition • Steroid drugs• Thin-layer chromatography Research ArticleChromatographic properties of five steroid drugs: cortisone, hydrocortisone, methylprednisolone, prednisolone and norgestrel have been studied by normal-, reversed-phase and hydrophilic neutral cyano-bonded silica stationary phase with five binary mobile phases (acetonitrile-water, acetonitrile-DMSO, acetonitrile-methanol, acetone-petroleum ether, acetone-water) in which the concentration of organic modifier was varied from 0 to 100% (v/v). This study reports the optimization of steroid hormones separation. Chromatographic retention data and possible retention mechanisms are discussed. Separation abilities of mobile and stationary phases were studied using the principal component analysis method. The best separation of methylprednisolone and prednisolone is with a chromatographic system included silica gel as stationary phase and mixture of acetonitrile and DMSO (10:90 v/v). These two anti-inflammatory drugs can be fast separated from norgestrel when CN is used as stationary phase and acetone and water (40:60 v/v) as mobile phase. The highest values of the parameter Δ(ΔG o ) and alfa for cortisone and hydrocortisone was observed in case of using CN as stationary phase and water-acetonitryle (40:60 v/v) as mobile phase.

Membranes with Functionalized Nanopores for Aromaticity-Based Separation of Small Molecules

“…53,55 Separation of hormones with similar chemical structures is essential for drug manufacture and in analytical applications for clinical diagnosis. 56,57 Thus, we selected two steroid hormones as an example of a pair of solutes with similar size and charge but different aromaticity. Estrone sulfate (ESTR), the most abundant estrogen precursor in blood, has an aromatic ring.…”