A simplified competitive protein-binding assay for adenosine 3',5'-monophosphate in plasma

Latner, A. L.; Prudhoe, Kenneth

doi:10.1016/0009-8981(73)90412-9

Cited by 22 publications

(4 citation statements)

References 9 publications

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“…The difference between these two antisera was examined by measuring iPTH with both of them in the plasma of a patient with chronic renal failure after chromatography with Biogel P-100 (50 x 1 cm). The cAMP was measured in plasma and in urine by a protein binding assay using the method of Latner & Prudhoe [29].…”

Section: Methodsmentioning

confidence: 99%

Nephrogenous cyclic AMP and plasma parathyroid hormone in hypercalcaemia: the influence of renal function

Bill¹,

Jacquet²,

Burckhardt³

1984

Eur J Clin Investigation

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Immunoreactive parathyroid hormone (PTH) levels and nephrogenous cyclic adenosine monophosphate (cAMP) have been reported to be useful parameters in the diagnosis of hyperparathyroidism. Measurements in hyperparathyroid patients usually give values above the normal range when PTH is measured with a carboxyterminal radioimmunoassay and when nephrogenous cAMP is related to glomerular filtration rate. We tested these two parameters in two groups of hypercalcaemic patients (twelve cases of primary hyperparathyroidism and fourteen cases of hypercalcaemia of non-parathyroid origin) and in two groups of normocalcaemic subjects (twenty-one young healthy volunteers and fourteen elderly subjects without parathyroid disease). Slight impairment of renal function caused elevated values of immunoreactive parathyroid hormone in a carboxyterminal radioimmunoassay and also of nephrogenous cAMP when related to glomerular filtration rate. We found that elevated nephrogenous cAMP without parathyroid disease could be attributed to renal insufficiency and to the mode of expression generally used for the nephrogenous cAMP.

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Section: Methodsmentioning

confidence: 99%

Nephrogenous cyclic AMP and plasma parathyroid hormone in hypercalcaemia: the influence of renal function

Bill¹,

Jacquet²,

Burckhardt³

1984

Eur J Clin Investigation

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“…All specimens were assayed blind. The detection-limit of this method is 0·25 pmol and its precision is ± 0·2 prnol (Latner and Prudhoe, 1973). Results were expressed as pmol cyclic-AMP per mg protein.…”

Section: Discussionmentioning

confidence: 99%

“…Cyclic-AMP was assayed by a protein-binding method, as previously described (Latner and Prudhoe, 1973). All specimens were assayed blind.…”

Section: Discussionmentioning

confidence: 99%

Cyclic Adenosine 3′, 5′ Monophosphate: A Possible Indicator of Premalignant Changes in the Large Bowel

Latner¹,

Turner²,

Tregoning

1979

Ann Clin Biochem

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Cyclic adenosine 3′, 5′ monophosphate (cyclic-AMP) has been estimated in mucosal biopsy samples removed from the descending colon and rectum at endoscopy to investigate the possibility of using this substance for monitoring pre-malignant changes in the large bowel. Four groups of patients have been studied: those with normal large bowel and rectal mucosa; those with non-malignant inflammatory bowel disease; those with an adenomatous polyp in the descending colon or sigmoid colon; and those with a rectal adenocarcinoma. No difference was found in the cyclic-AMP content of ‘normal’ rectal mucosa, ‘normal’ colonic mucosa, ‘diseased’ colonic mucosa, carcinomas, and uninvolved mucosa adjacent to the polyps. Less cyclic-AMP was found in the polyps than in adjacent uninvolved mucosa. Conversely, more cyclic-AMP was found in the carcinomas than in adjacent uninvolved mucosa. It is concluded that although cyclic-AMP may be a very useful parameter for delineating the extent of the disease in individual patients, it is not a suitable biochemical marker for the screening of neoplastic changes in the large bowel in the population as a whole.

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“…The recovery of cyclic nucleotides from the extraction and separation procedures was monitored using trace quantities of radioactive cyclic AMP and cyclic GMP. The levels of cyclic AMP and cyclic GMP were measured using protein-binding methods (10,11). Urinary levels of creatinine were determined by a modifi cation of the Jaffe reaction (12) using a Boehringer Mannheim Test Combination.…”

Section: Methodsmentioning

confidence: 99%

Adenosine 3’ ,5’-Cyclic Monophosphate and Guanosine 3’,5’-Cyclic Monophosphate Levels in Tumour, Plasma and Urine during the Growth of a Fibrosarcoma in Hamsters

Latner¹,

Patterson²,

Turner³

1980

Pathobiology

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Levels of cyclic AMP and cyclic GMP were measured in tumours, plasmas, and urines from groups of hamsters at different times after implantation of a fibrosarcoma (of kidney cell origin) and in kidney cortices, plasmas and urines from healthy hamsters. Initially, tumour cyclic AMP levels were significantly lower than those in kidney cortex but this difference disappeared during the growth of the tumour. Plasma and urine cyclic AMP levels were significantly depressed during all phases of tumour growth. In general, cyclic GMP levels were unaffected by the presence of a tumour except when hamsters carried a very high tumour load.

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A simplified competitive protein-binding assay for adenosine 3',5'-monophosphate in plasma

Cited by 22 publications

References 9 publications

Nephrogenous cyclic AMP and plasma parathyroid hormone in hypercalcaemia: the influence of renal function

Nephrogenous cyclic AMP and plasma parathyroid hormone in hypercalcaemia: the influence of renal function

Cyclic Adenosine 3′, 5′ Monophosphate: A Possible Indicator of Premalignant Changes in the Large Bowel

Adenosine 3’ ,5’-Cyclic Monophosphate and Guanosine 3’,5’-Cyclic Monophosphate Levels in Tumour, Plasma and Urine during the Growth of a Fibrosarcoma in Hamsters

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