A simplified medium for the in vitro culture of mother sporocysts of the schistosomeTrichobilharzia ocellata

Schallig, Henk D. F. H.; Schut, Alexandria; Knaap, W.P.W. van der; Jong-Brink, Marijke de

doi:10.1007/bf00930825

Cited by 20 publications

(3 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Miracidia, cercariae and schistosomula were prepared as described previously [44]. Miracidia were transformed in vitro into sporocysts by incubation in Minimum Salt Medium at 28 °C for 18 h [45]. Adult schistosomes were collected by portal perfusion of infected hamsters.…”

Section: Methodsmentioning

confidence: 99%

Diversification of the insulin receptor family in the helminth parasite Schistosoma mansoni

et al. 2007

View full text Add to dashboard Cite

The complexification of cell to cell interactions along metazoan evolution has led to the development of a molecular network specialized in intercellular communication. Receptor tyrosine kinases (RTKs) are among the most ancient and important proteins that emerged in multicellular organisms. RTKs form a superfamily of transmembrane receptors that bind a large panel of ligands including hormones, growth factors and cytokines, and trigger different signalling cascades inside the cell for the control and regulation of key cellular processes. In eumetazoan organisms, RTK mediation is essential for embryogenesis [1,2], growth [3] and metabolic regulations [4]. RTK diversity increased along with speciation, possibly due to the acquisition of new regulatory processes in cell function, so that conserved and original classes of RTK could be found in different metazoan organisms [5][6][7]. RTKs possess a unique transmembrane domain and their capacity to transduce signals inside the cell is dependent on their autophosphorylation and requires vicinity of two tyrosine kinase (TK) domains. Members of the insulin receptor (IR) family belong to the very well conserved RTK class II, and were probably present in the most ancient metazoans because they have been identified in diploblastic organisms like sponge [8]. Although most of the RTK are monomeric and dimerize upon ligand binding, IR are constitutively assembled in a 2 b 2 heterotetramers [9,10]. Binding of insulin peptides

show abstract

Section: Methodsmentioning

confidence: 99%

Diversification of the insulin receptor family in the helminth parasite Schistosoma mansoni

et al. 2007

View full text Add to dashboard Cite

show abstract

“…Flasks which were 80% confluent were extensively washed in minimal salt saline (MSM) medium (Schallig et al 1990). Successive washing media were analysed by SDS-PAGE in order to check for the presence of residual serum molecules.…”

Section: Methodsmentioning

confidence: 99%

Gene expression changes in Schistosoma mansoni sporocysts induced by Biomphalaria glabrata embryonic cells

Coppin

Lefebvre

Caby

et al. 2003

Parasitology Research

View full text Add to dashboard Cite

Biomphalaria glabrataembryonic (Bge) cells have been shown to provide favourable environmental conditions for the development of Schistosoma mansoni sporocysts. We investigated the effect of Bge excretory-secretory products on metabolic activity and gene transcription in S. mansoni mother sporocysts. Using the differential-display technique, we identified several sporocyst transcripts regulated by exposure to Bge soluble components. Research in databases indicated that six of the eight differential products analysed were homologous to sequences already present in databases. Two transcripts appeared of interest for schistosome development since they could be associated with cell division and protein synthesis in developing sporocysts. Their up-regulation following contact with cell products was confirmed by semi-quantitative RT-PCR. The first fragment coded for a part of the chaperonin containing T-complex protein gamma subunit-like protein of S. mansoni (SmTCP 1-C). The second one represented a new S. mansoni expressed sequence tag encoding a protein homologous to various glutaminyl-tRNA synthetases (GlnRS). The full-length sequence of SmGlnRS was cloned from adult schistosomes and its primary sequence was compared to other GlnRS. The overexpression of SmTCP-1 and SmGlnRS could be correlated with the metabolic changes observed in Bge-exposed sporocysts.

show abstract

“…Firstly, it is rather difficult to administer parasitic factors which might have rather long-term effects on the development of "Anlagen" transplanted into NP snails. As far as this last point is concerned, we have found a way of administering parasitic factors by means of a slow release pellet (SRP) consisting of cacao butter to which products were added that had been excretedtsecreted (EIS products) by in vitro cultured miracidia during their transformation into mother sporocysts [see Schallig et al (1990)l. Secondly, although the data obtained 5 days after transplantation (see Table 1) suggest that the percentage of PCNA positive myoblasts is lower in the presence of EIS products, it appeared to be difficult to obtain significant differences between NP snails with an "Anlage" and only an empty SRP implanted and those with an "Anlage" and a SRP containing EIS products.…”

Section: Transplantation Experimentsmentioning

confidence: 99%

Inhibition of the development of the reproductive tract in parasitized snails

Jong-Brink¹,

Wal²,

Laak³

et al. 1999

Invertebrate Reproduction & Development

View full text Add to dashboard Cite

Myoblasts, muscle cells with the capacity to divide, have been detected in "Anlagen" of the male copulation organ of Lymnaea stagnalis. They only occur in the apical part of the penis. Here they could be found throughout life. Mitotic activity of these cells can be demonstrated by using an antiserum to a S-phase specific cell cycle marker, PCNA [see, e.g., Baserga (1991)l. The numberlpercentage of PCNA positive myoblasts is a good parameter for growth of this male copulation organ and hence also for inhibition of its growth and development as occurs in parasitized snails. In transplantation experiments, "Anlagen" of the copulation organ were used from snails 7-9 weeks after being parasitized as they can be excised in this stage and transplanted into either parasitized or nonparasitized snails. These experiments have indicated that humoral, parasitic excretorylsecretory factors can be responsible for the inhibition of growth and .differentiation of the copulation organ in parasitized snails as reflected by a relatively low number of PCNA positive myoblasts compared to the controls. Data obtained in in vitro experiments showed a significant decrease of the number of myoblasts in "Anlagen" cultured in the presence of parasitic E/S products. The fact that no significant effect was found on the relative low number of PCNA positive myoblasts is discussed. The effect of parasitic E/S products on these myoblasts appeared to be exerted in a direct way, not mediated by CNSderived factors or by factors from cells in the connective tissue sheath around the CNS. Although it appears possible to use transplantation and/or in vitro culturing of these "Anlagen" as a bioassay for identification of the parasitic factor@) responsible for the inhibitory effects on myoblasts, the methods are very laborious and do not seem very appropriate for testing many fractions of EIS products.

show abstract

A simplified medium for the in vitro culture of mother sporocysts of the schistosomeTrichobilharzia ocellata

Cited by 20 publications

References 13 publications

Diversification of the insulin receptor family in the helminth parasite Schistosoma mansoni

Diversification of the insulin receptor family in the helminth parasite Schistosoma mansoni

Gene expression changes in Schistosoma mansoni sporocysts induced by Biomphalaria glabrata embryonic cells

Inhibition of the development of the reproductive tract in parasitized snails

Contact Info

Product

Resources

About