A Simplified Method for the Estimation of Total Cholesterol in Serum and Demonstration of Its Specificity

Abell, Liese L.; Levy, Betty B.; Brodie, Bernard B.; Kendall, Forrest E.

doi:10.1016/s0021-9258(19)50907-3

Cited by 2,840 publications

(91 citation statements)

References 4 publications

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“…14 For the present investigation, hypercholesterolemia was defined as a clinic blood cholesterol level of 240 mg/dL (6.2 mmol/L) or greater or reported use of lipid-lowering therapy since the last clinic examination. 15,16 Women who reported no menstrual periods during the 12 months prior to a clinic examination were considered menopausal from that time forward. Data from subsequent examinations were used to reclassify subjects according to their current age, BMI, diabetes mellitus status, serum cholesterol level, cigarette smoking, and hypertension status.…”

Section: Participants and Methodsmentioning

confidence: 99%

Agricultural Subsidies

Grandi¹,

Perrin²

2012

Arch Intern Med

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Background: To our knowledge, no single investigation concerning the long-term effects of overweight status on the risk for hypertension, hypercholesterolemia, diabetes mellitus, and cardiovascular sequelae has been reported.Methods: Relations between categories of body mass index (BMI), cardiovascular disease risk factors, and vascular disease end points were examined prospectively in Framingham Heart Study participants aged 35 to 75 years, who were followed up to 44 years. The primary outcome was new cardiovascular disease, which included angina pectoris, myocardial infarction, coronary heart disease, or stroke. Analyses compared overweight (BMI [calculated as weight in kilograms divided by the square of height in meters], 25.0-29.9) and obese persons (BMI Ն30) to a referent group of normal-weight persons (BMI, 18.5-24.9). Results:The age-adjusted relative risk (RR) for new hypertension was highly associated with overweight sta-tus (men: RR, 1.46; women: RR, 1.75). New hypercholesterolemia and diabetes mellitus were less highly associated with excess adiposity. The age-adjusted RR (confidence interval [CI]) for cardiovascular disease was increased among those who were overweight (

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Section: Participants and Methodsmentioning

confidence: 99%

Agricultural Subsidies

Grandi¹,

Perrin²

2012

Arch Intern Med

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“…The cholesterol [28], TG [29], Cr [30] and glucose [25] level were determined by enzymatic colorimetric test. The total protein was measured using colorimetric test.…”

Section: Plasmaenzyme Activity and Metabolite Levelmentioning

confidence: 99%

Biochemical Alteration Induced by Cadmium and Lead in Common Carp via an Experimental Food Chain

Mohiseni¹,

Asayesh²,

Bazarnoie³

et al. 2016

IJT

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Background: Evaluation on the toxicity of two mainly contaminant heavy metals, cadmium (Cd) and lead (Pb) through the food chain was the aim of this study. Methods: A total number of 270 healthy common carp (4±1.14 g) in April, 2015 transported to the Khatam Alanbia University of Technology, Behbahan, Iran. Fishes were divided into three groups and transferred to the 20 L aquaria each containing 30 juveniles. The first group (control) fed by metal-free Artemia fransiscan anauplii throughout the experiment. The second and third groups were feeding by Cd and Pb (1.5 mg/L free ion) contaminated nauplia, respectively. The experimental study was carried out for three weeks and sampling was done in 4th, 7th, 14th and 21st days. Finally, the alterations in plasma biochemical responses were determined. Results: Alanine aminotransferase and aspartate aminotransferase activities increased in response to feeding Pb-contaminated nauplia. Creatine phosphokinase activity showed significant increase in fourth day about both Cd and Pb and at the end of experiment only in Cd treatment (P<0.05). Cholesterol and triglyceride were increased significantly only for Pb (P<0.05). Plasma glucose and creatinine levels increased by both heavy metals compared to the control but glucose just remained high only for Pb at the end of the experiment. Total protein, albumin and globulin were significantly declined in both metal contaminated groups (P<0.05). Conclusion: It seems Pb had a greater toxicity than Cd through the food chain and it may be due to its more trophic transfer than Cd.

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“…Two consecutive measurements were made and the average was used in these analyses. Total cholesterol was measured by using the colorimetric method of Sperry [9] for the first survey, but the Abell-Kendall method for all subsequent surveys [10].…”

Section: Study Populationsmentioning

confidence: 99%

Regression Dilution Bias in Blood Total and High-Density Lipoprotein Cholesterol and Blood Pressure in the Glostrup and Framingham Prospective Studies

Lewington

Thomsen

Davidsen

et al. 2003

European Journal of Cardiovascular Prevention & Rehabilitat

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Background In epidemiological studies, within-person variability in measured values of a risk factor may underestimate the association between prolonged or ‘usual’ levels of a risk factor with risk of disease — ‘regression dilution’. The importance of regression dilution for high-density lipoprotein (HDL)-cholesterol and the extent to which this may differ from that for total cholesterol is not known. The aim of this study was to assess the magnitude of regression dilution bias for HDL-cholesterol, total cholesterol and blood pressure after varying intervals of follow-up in two prospective cohort studies. Methods Regression dilution ratios were estimated for each risk factor using the correlations between baseline and re-survey values in the Glostrup Population Studies and the NHLBI Framingham Heart Study after various time intervals. The regression dilution ratios in each cohort after a fixed interval between measurements were compared. Results The regression dilution ratios after 10 years were 0.51 and 0.56 for systolic blood pressure in Glostrup and Framingham, respectively; 0.52 and 0.54 for diastolic blood pressure; and 0.68 and 0.63 for total cholesterol. In both studies, the regression dilution ratios for these risk factors became more extreme with increasing intervals between measurements. The regression dilution ratio for HDL-cholesterol after 10 years in Glostrup was 0.72, which suggests that the importance of regression dilution for HDL-cholesterol was similar to that for total cholesterol. Conclusion Failure to correct for increasing regression dilution with longer follow-up may account for some of the discrepant results obtained for the importance of these risk factors in epidemiological studies at varying intervals of follow-up.

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A Simplified Method for the Estimation of Total Cholesterol in Serum and Demonstration of Its Specificity

Cited by 2,840 publications

References 4 publications

Agricultural Subsidies

Agricultural Subsidies

Biochemical Alteration Induced by Cadmium and Lead in Common Carp via an Experimental Food Chain

Regression Dilution Bias in Blood Total and High-Density Lipoprotein Cholesterol and Blood Pressure in the Glostrup and Framingham Prospective Studies

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