A Simplified Procedure for the Isolation of Bile Acids from Serum Based on a Batch Extraction with the Non-Ionic Resin—Amberlite XAD-7

Barnes, Stephen; Chitranukroh, A.

doi:10.1177/000456327701400163

Cited by 46 publications

(8 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…213 M-(2x TMSiOH + Ring D + S.C.) 20 17 281 M-(3x TMSiOH + C-22 -C-24) 14 19 229 M-(2x TMSiOH + C-17 + C-16 + S.C.) 10 11 343 M-(2x TMSiOH + S.C) 19 17 243 C-3-C-7 15 21 368 M-(3x TMSiOH) 38 22 249 M-(Ring A + Ring B) 14 24 458 M-(2x TMSiOH) 29 18 255 M-(2x TMSiOH + S.C.) 23 (26,27). This suggests that shunting of blood past the liver cannot be the explanation and that deficiency in the transport processes is probably occurring.…”

Section: Discussionmentioning

confidence: 99%

“…They were then placed in 5-ml reactivials (Reliance Glassworks, Denville, Ill.), sealed with a cap containing a teflon liner and heated to 110°C overnight to hydrolyze the bile salt conjugates. Each hydrolysate was diluted with 9 ml distilled water and extracted with XAD-7 resin (19). The methanol eluates were evaporated to dryness and treated with 1 ml 2,2'-dimethoxypropane and 100 Al of 1 M-HCI (20) to cause solvolysis and formation of methyl esters.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Postnatal physiologic hypercholemia in both premature and full-term infants.

Barnes¹,

Berkowitz²,

Hirschowitz³

et al. 1981

J. Clin. Invest.

View full text Add to dashboard Cite

A B S T R A C T Previous studies have shown that bile salt concentrations in human blood taken from the placenta at birth ofterm infants are in the range found in adults. A 1251-radioimmunoassay procedure and capillary gas liquid chromatography-mass spectrometry have been used in this investigation to measure serum bile salt concentrations in premature and normal term infants. It was found that the serum bile salt concentration in samples taken at birth in premature infants were also similar to that of adults. In the week after birth the serum bile salt concentration rose four-to sevenfold in each of the infant groups. The increase was independent of gestational age and the "health" of the child. A similar increase was observed in term infants. Thus, hypercholemia is physiologic in newborn infants. In conjunction with other abnormalities of the enterohepatic circulation of bile salts there are profound implications in the newborn for the metabolism and excretion of those endogenous and exogenous substances that are dependent on the secretion of bile salt by the liver. In addition, speculations concerning the role of parenteral nutrition in the induction of cholestasis in premature infants should be made with caution.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Postnatal physiologic hypercholemia in both premature and full-term infants.

Barnes¹,

Berkowitz²,

Hirschowitz³

et al. 1981

J. Clin. Invest.

View full text Add to dashboard Cite

show abstract

“…Echinocytosis by bile salts or lysolecithin, and its reversal by addition of normal HDL, was rapid and the echinocytes produced resembled by SEM those generated by patient HDL. However, HDL-induced echinosytosis could not be explained by detectable increases ofthese amphipaths in active HDL; freshly isolated echinocytic HDL contained no more lysolecithin than normal HDL (1-2% of total phospholipid) and had a low total bile acid content (2-9 nmol/mg protein) when compared to inactive HDL from four patients with extrahepatic obstructive jaundice (1 [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17] give complete reversal to discocytes (B). Echinocytes from a patient with alcoholic cirrhosis also returned towards a normal erythrocyte shape when similarly incubated with normal HDL; the percentage of echinocytes II and III present were reduced from 14% (C) to zero (D).…”

Section: And E)mentioning

confidence: 99%

Erythrocyte echinocytosis in liver disease. Role of abnormal plasma high density lipoproteins.

Owen¹,

Brown²,

Harry³

et al. 1985

J. Clin. Invest.

View full text Add to dashboard Cite

Echinocytes were frequently found in patients with liver disease when their blood was examined in wet films, but rarely detected in dried, stained smears. When normal erythrocytes (discocytes)were incubated with physiologic concentrations of the abnormal high density lipoproteins (HDL) from some jaundiced patients, echinocytosis developed within seconds. Other plasma fractions were not echinocytogenic. There was a close correlation between the number of echinocytes found in vivo and the ability of the corresponding HDL to induce discocyte-echinocyte transformation. On incubation with normal HDL, echinocytes generated in vitro rapidly reverted to a normal shape, and echinocytes from patients showed a similar trend. Echinocytosis occurred without change in membrane cholesterol content, as did its reversal, and was not caused by membrane uptake of lysolecithin or bile acids. Abnormal, echinocytogenic HDL showed saturable binding to -5,000 sites per normal erythrocyte with an association constant of 108 M-'. Nonechinocytogenic patient HDL and normal HDL showed only nonsaturable binding. Several minor components of electrophoretically separated erythrocyte membrane proteins bound the abnormal HDL; pretreatment of the cells with trypsin or pronase reduced or eliminated binding. Echinocytosis by abnormal HDL required receptor occupancy, rather than transfer of constituents to or from the membrane, because cells reversibly prefixed in the discoid shape by wheat germ agglutinin, and then exposed to abnormal HDL, did not become echinocytes when the HDL and lectin were successively removed. Binding did not cause dephosphorylation of spectrin. We conclude that the echinocytes of liver disease are generated from discocytes by abnormal HDL, and we infer that the shape change is mediated by cell-surface receptors for abnormal HDL molecules.

show abstract

“…These studies were performed at least one Croydon, Surrey) by a batch method (Barnes and week before the standard study in the fasting state. Chitranukroh, 1977). The plasma 'total' bile Measurement of radioactivity in plasma (1 ml) acid concentration was determined by a modifiafter its addition to 12 ml NE-260 scintillation fluid cation of the fluorimetric 3-hydroxysteroid dehydro-(Nuclear Enterprises, Edinburgh, Scotland) was genase method of Murphy et al (1970).…”

Section: Subjectsmentioning

confidence: 99%