A simplified protocol for micropropagation of guayule (Parthenium argentatum gray)

Abstract

A simple, efficient protocol for in vitro micropropagation of guayule is reported. Shoot cultures were maintained on MS (Murashige and Skoog, 1962) medium supplemented with 1.0 mg l Ϫ1 (4.4 mM) 6-benzylaminopurine and 0.025 mg l Ϫ1 (0.13 mM) a-naphthaleneacetic acid. Excised shoots were treated for 14-18 h with 100 mg l Ϫ1 (492.1 mM) indole-3-butyric acid in 0X5 Â MS salts to induce rooting. The shoots were subsequently inserted into cellulose plugs which were packed in sterile, ventilated plastic culture vessels and moistened with 0X5 Â MS medium without growth regulators. Use of cellulose plugs, liquid medium and ventilated culture vessels facilitated acclimation. Rooted shoots were transplanted into potting medium and acclimated to greenhouse conditions by covering with a cloche for 2 d, followed by daily watering for the first week.

Micropropagation of Medlar (Mespilus germanica L.), A Mediterranean Fruit Tree

“…However, in many investigations, maximum roots were formed when medium was supplemented with IBA (Dinesh et al, 2019;Fan et al, 2017;Kereša et al, 2012;Mahipal et al, 2016;Nand et al, 2004;Prakash et al, 2006;Sharma and Vashistha, 2015c;Sulusoglu and Cavusoglu, 2013;Yildirim, 2012). Castillón and Cornish (2000) revealed that IBA was most effective auxin for induction of roots than IAA and NAA in Parthenium argentatum, a woody desert shrub. Venkatachalam et al (2015) showed that among the three auxins (IAA, NAA and IBA) used for in vitro rooting of the cultured shoots of Bambusa arundinacea, IBA was the most suitable followed by NAA.…”