A simultaneous determination of norephedrine, pseudoephedrine, ephedrine and methylephedrine in Ephedrae Herba and oriental pharmaceutical preparations by ion-pair high-performance liquid chromatography.

Sagara, Kazuhiko; Oshima, Toshiyuki; Misaki, Tetsuo

doi:10.1248/cpb.31.2359

Cited by 60 publications

(36 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The analytical condition used in HPLC 8 to determine the ephedrine content in the Ephedra herb is directly applicable to the determination of the synephrine content in the peel of Citrus fruit and immature Citrus fruit. 5 Accordingly, referring to the reports 11,12 on the analysis of ephedrine by using CE, these analytical conditions were used to examine the synephrine content of Citrus fruits.…”

Section: Resultsmentioning

confidence: 99%

“…The conditions used were as follows: a high-pressure machine [Model LP-1000 (Eyela)]; a detector [Model UV-9000 (Eyela, wavelength 220 nm)]; a column [LiChrospher RP-18(e) (Merck, 4 mm i.d.×25 cm)]; a mobile phase (conformed to the literature 8 ), water-acetonitrile-sodium dodecyl sulfate-phosphoric acid (65:35: 0.5:0.1); a flow rate of 1.0 ml/min. The chromatograms were recorded on a SIC Chromatocorder 12, and the peak areas were compared for quantitative determinations.…”

Section: High-performance Liquid Chromatographymentioning

confidence: 99%

See 1 more Smart Citation

Analysis of Synephrine in the Peel of Citrus Fruit, Immature Citrus Fruit and Decoctions of Chinese Medicinal Prescriptions Containing These Crude Drugs by Capillary Electrophoresis

1999

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

Section: High-performance Liquid Chromatographymentioning

confidence: 99%

Analysis of Synephrine in the Peel of Citrus Fruit, Immature Citrus Fruit and Decoctions of Chinese Medicinal Prescriptions Containing These Crude Drugs by Capillary Electrophoresis

1999

View full text Add to dashboard Cite

“…In GC analysis, chemical derivatization was wildly applied to enhance volatility and detection efficiency [20]. However, more than one derivative might be obtained for the same compound [21]. Therefore, our team performed a method without derivatization for the simultaneous determination of ephedra alkaloids in urine by GC-MS in 2005 [22].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of ephedra alkaloids in traditional chinese medicines by high-performance liquid chromatography

Chen¹,

Li²,

Chen³

et al. 2012

Acta Chromatographica

View full text Add to dashboard Cite

Summary.A rapid, simple, and practical high-performance liquid chromatographic method (HPLC) was developed and validated for the simultaneous determination of norephedrine (NME), norpseudoephedrine (NMP), ephedrine (E), pseudoephedrine (PE), and methylephedrine (ME) in traditional Chinese medicines (TCM) which contained Ephedrae Herba (Ephedra). This analysis could be accomplished within 12.5 min with an Alltima Phenyl Column by isocratic elution using a mixture of KH 2 PO 4 (20 mM)-acetonitrile (96:4, v/v) as the mobile phase at a flow-rate of 0.6 mL min −1 and a wavelength of 210 nm. This method was successfully applied to quantify ephedra alkaloids in both Ma-xing-gan-shi decoction and Ephedra decoction. The concentration of total ephedra alkaloids (4.62 mg mL −1 ) in Ma-xing-gan-shi decoction was much lower than that (7.10 mg mL −1 ) in Ephedra decoction. Furthermore, the concentration of NME, NMP, E, PE, and ME was significantly lower in Ma-xing-gan-shi decoction than that in Ephedra decoction, respectively. The method was easily acceptable and would be popular with most analytical laboratories.

show abstract

“…These pharmacological actions appear to be due to a series of ephedra alkaloids such as l-ephedrine, d-pseudoephedrine (PSE), l-methylephedrine, and l-norephedrine (2 -4). Among these, l-ephedrine and PSE account for about 90% of the total alkaloids, and they are considered to play the principle role in the pharmacological actions of Ephedrae herba (4).…”

Section: Introductionmentioning

confidence: 99%

Effect of d-Pseudoephedrine on Cough Reflex and Its Mode of Action in Guinea Pigs

Minamizawa¹,

Goto²,

Ohi³

et al. 2006

J Pharmacol Sci

View full text Add to dashboard Cite

Abstract. d-Pseudoephedrine (PSE) is one of the main ingredients of Ephedrae herba. Although PSE is widely applied for patients with a common cold and upper respiratory inflammation as a decongestant, the effects of PSE on cough have never been reported. In this study, we investigated the antitussive effects of intraperitoneal injection of PSE on the cough reflex induced by microinjection of citric acid into the larynx of guinea pigs. PSE decreased the number of cough reflexes dose-dependently (−18.3 ± 5.0% at 20 mg / kg, P<0.05; −41.1 ± 7.2% at 60 mg / kg, P<0.01). Furthermore, PSE (60 mg / kg) increased the threshold intensity for inducing fictive cough by electrical micro-stimulation of the nucleus tractus solitarius (+72.7 ± 8.4%, P<0.01). On the afferent discharge of the superior laryngeal nerve, PSE suppressed the increases of amplitude and frequency when stimulated by citric acid at laryngeal mucosa. These results demonstrate that PSE possesses an antitussive effect that might be derived from both central and peripheral actions.

show abstract

A simultaneous determination of norephedrine, pseudoephedrine, ephedrine and methylephedrine in Ephedrae Herba and oriental pharmaceutical preparations by ion-pair high-performance liquid chromatography.

Cited by 60 publications

References 0 publications

Analysis of Synephrine in the Peel of Citrus Fruit, Immature Citrus Fruit and Decoctions of Chinese Medicinal Prescriptions Containing These Crude Drugs by Capillary Electrophoresis

Analysis of Synephrine in the Peel of Citrus Fruit, Immature Citrus Fruit and Decoctions of Chinese Medicinal Prescriptions Containing These Crude Drugs by Capillary Electrophoresis

Simultaneous determination of ephedra alkaloids in traditional chinese medicines by high-performance liquid chromatography

Effect of d-Pseudoephedrine on Cough Reflex and Its Mode of Action in Guinea Pigs

Contact Info

Product

Resources

About