A simultaneous process of 3D magnesium phosphate scaffold fabrication and bioactive substance loading for hard tissue regeneration

Lee, Jongman; Farag, Mohammad M.; Park, Eui Kyun; Lim, Jeong-Ok; Yun, Hui-suk

doi:10.1016/j.msec.2013.12.007

Cited by 68 publications

(65 citation statements)

References 32 publications

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“…However, in pathological fractures, traumatic bone loss or primary tumour resection, where the bone defect exceeds a critical size, bone is no longer able to heal itself [1,2] . Additionally, this regenerative ability reduces with age [3] .…”

Section: Introductionmentioning

confidence: 99%

Morphological, mechanical and biological assessment of PCL/pristine graphene scaffolds for bone regeneration

Wang¹,

Caetano²,

Chiang³

et al. 2024

IJB

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Scaffolds are physical substrates for cell attachment, proliferation, and differentiation, ultimately leading to the regeneration of tissues. They must be designed according to specific biomechanical requirements such as mechanical properties, surface characteristics, biodegradability, biocompatibility, and porosity. The optimal design of a scaffold for a specific tissue strongly depends on both materials and manufacturing processes. Polymeric scaffolds reinforced with electro-active particles could play a key role in tissue engineering by modulating cell proliferation and differentiation. This paper investigates the use of an extrusion additive manufacturing system to produce PCL/pristine graphene scaffolds for bone tissue applications. PCL/pristine graphene blends were prepared using a melt blending process. Scaffolds with regular and reproducible architecture were produced with different concentrations of pristine graphene. Scaffolds were evaluated from morphological, mechanical, and biological view. The results suggest that the addition of pristine graphene improves the mechanical performance of the scaffolds, reduces the hydrophobicity, and improves cell viability and proliferation.

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Section: Introductionmentioning

confidence: 99%

Morphological, mechanical and biological assessment of PCL/pristine graphene scaffolds for bone regeneration

Wang¹,

Caetano²,

Chiang³

et al. 2024

IJB

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“…However, in pathological fractures, traumatic bone loss or primary tumour resection, when the defect exceeds a critical size, the bone is no longer able to heal itself [1,2]. In these cases, the clinical approach is the use of bone grafts, defined as an implanted material that promotes healing on its own or in combination with other materials, through osteogenesis, osteoinduction and osteoconduction [3].…”

Section: Introductionmentioning

confidence: 99%

“…In these cases, the clinical approach is the use of bone grafts, defined as an implanted material that promotes healing on its own or in combination with other materials, through osteogenesis, osteoinduction and osteoconduction [3]. Bone grafts can be divided into autografts, allografts and xenografts [1][2][3][4][5][6]. Autografts, harvested from one site and implanted into another site within the same individual, are osteogenic, osteoinductive and osteoconductive, and do not present risk of disease transmission or immune system rejection [2].…”

Section: Introductionmentioning

confidence: 99%

Cellularized versus decellularized scaffolds for bone regeneration

et al. 2016

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An optimal scaffold based strategy for in vivo repair of large bone defects and its associated problems is presented in this work. Three polymeric scaffolds produced by using an extrusion-based additive manufacturing system were examined in a rat critical bone defect model: scaffolds without cells, with undifferentiated Adipose-derived mesenchymal stem cells (ADSCs) and differentiated ADSCs (osteoblasts). Scaffolds with undifferentiated cells seem to be the best strategy as they exhibited around 22% more bone formation than natural bone healing, and around 15% more than the two other cases.Authors observed that scaffolds enabled cell migration and tissue formation. Results suggest that undifferentiated ADSCs strongly contribute to new bone formation with no rejection if scaffolds are used to support cell migration, proliferation and differentiation. Our long-term goal is to engineer high-quality cell seeded-scaffolds (autograft and allograft) for bone regeneration, mainly in elderly patients.

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“…[9][10][11] In our previous studies, we developed a novel roomtemperature process of three-dimensional (3D) magnesium phosphate (MgP) scaffold fabrication, using a paste-extruding deposition (PED) system. 12 This technique enabled us to directly blend lysozyme, as a model bioactive substance, into MgP powder, to introduce homogeneous distribution in the scaffold. This was completely ascribed to a simple cementation process at room temperature, instead of the typical sintering process at high temperature.…”

Section: Introductionmentioning

confidence: 99%

“…12 Briefly, Mg(OH) 2 (Junsei Chemical Co., Ltd., Tokyo, Japan) and H 3 PO 4 (DC Chemical Co., Ltd., Seoul, Korea) were reacted with a molar ratio of 3 M to 2 M. Then, 2 M of H 3 PO 4 was added to a 3 M of Mg(OH) 2 suspension in a dropwise manner. The solution was mixed uniformly for 10 hours and left for 24 hours to age.…”

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confidence: 99%

Effect of hydroxyapatite-containing microspheres embedded into three-dimensional magnesium phosphate scaffolds on the controlled release of lysozyme and in vitro biodegradation

Lee¹,

Yun²

2014

IJN

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The functionality of porous three-dimensional (3D) magnesium phosphate (MgP) scaffold was investigated for the development of a novel protein delivery system and biomimetic bone tissue engineering scaffold. This enhancement can be achieved by incorporation of hydroxyapatite (HA)-containing polymeric microspheres (MSs) into a bulk MgP matrix, and a paste-extruding deposition (PED) system. In this work, the amount of MS and HA was precisely controlled when manufacturing MS-embedded MgP (MS/MgP) composite scaffolds. The main influence was researched in terms of in vitro lysozyme-release, in vitro biodegradation, mechanical properties, and in vitro calcification. The controlled release of lysozyme was indicated, while showing graded release patterns according to HA content. The composite scaffolds degraded gradually with MS content and degradation time. Due to the effect of HA inclusion, the higher HA-containing MS/MgP scaffolds could, not only delay the biodegradation process but also, compensate for the possible loss of mechanical properties. In this regard, it is reasonable to confirm the inverse relationship between biodegradation and corresponding compressive properties. In order to encourage bioactivity and osteoconductivity, the MS/MgP composite scaffolds were subjected to simulated body fluid treatment. Calcium deposition was, in turn, improved with increasing MS and HA content over time. This quantitative result was also proved using morphological and elemental analysis. In summary, a significant transformation of a monolithic MgP scaffold was directed toward a multifunctional bone tissue engineering scaffold equipped with controlled protein delivery, biodegradability, and bioactivity.

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A simultaneous process of 3D magnesium phosphate scaffold fabrication and bioactive substance loading for hard tissue regeneration

Cited by 68 publications

References 32 publications

Morphological, mechanical and biological assessment of PCL/pristine graphene scaffolds for bone regeneration

Morphological, mechanical and biological assessment of PCL/pristine graphene scaffolds for bone regeneration

Cellularized versus decellularized scaffolds for bone regeneration

Effect of hydroxyapatite-containing microspheres embedded into three-dimensional magnesium phosphate scaffolds on the controlled release of lysozyme and in vitro biodegradation

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A simultaneous process of 3D magnesium phosphate scaffold fabrication and bioactive substance loading for hard tissue regeneration

Cited by 68 publications

References 32 publications

Morphological, mechanical and biological assessment of PCL/pristine graphene scaffolds for bone regeneration

Morphological, mechanical and biological assessment of PCL/pristine graphene scaffolds for bone regeneration

Cellularized versus decellularized scaffolds for bone regeneration

Effect of hydroxyapatite-containing microspheres embedded into three-dimensional magnesium phosphate scaffolds on the controlled release of&nbsp;lysozyme and in vitro biodegradation

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Effect of hydroxyapatite-containing microspheres embedded into three-dimensional magnesium phosphate scaffolds on the controlled release of lysozyme and in vitro biodegradation