A single dose of recombinant Salmonella typhimurium induces specific humoral immune responses against heterologous Eimeria tenella antigens in chicken

Pogonka, Thomas; Klotz, Christian; Kovács, Ferenc; Lucius, Richard

doi:10.1016/s0020-7519(02)00251-5

Cited by 50 publications

(21 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Development of a protective vaccine against this disease, although very challenging, is a desirable approach for decreasing its negative economic impact and for improving the welfare of poultry. A number of RASV strains have been developed and are able to bear and express several Eimeria antigens for induction of antigen-specific immune responses that ultimately lead to protection against challenge by this parasite (20,27). Thus far, none of these antigens have been found to fully protect against coccidiosis.…”

Section: Discussionmentioning

confidence: 99%

“…The use of antigens specific to two different developmental stages, sporozoites (SO7) and merozoites (EAMZ250), may lead to more complete immunity against the parasite. Other workers have delivered the SO7 antigen via attenuated Salmonella strains and shown that there was induction of antibody titers (immunoglobulin G [IgG] and IgA) to this antigen in immunized chickens, although no cell-mediated immune responses or Eimeria challenge studies were described (27).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Immunogenicity of Recombinant Attenuated Salmonella enterica Serovar Typhimurium Vaccine Strains Carrying a Gene That Encodes Eimeria tenella Antigen SO7

Konjufca¹,

Jenkins

Wang³

et al. 2008

Infect Immun

View full text Add to dashboard Cite

Recombinant attenuated Salmonella vaccines against avian coccidiosis were developed to deliver Eimeria species antigens to the lymphoid tissues of chickens via the type 3 secretion system (T3SS) and the type 2 secretion system (T2SS) of Salmonella. For antigen delivery via the T3SS, the Eimeria tenella gene encoding sporozoite antigen SO7 was cloned downstream of the translocation domain of the Salmonella enterica serovar Typhimurium sopE gene in the parental pYA3868 and pYA3870 vectors to generate pYA4156 and pYA4157. Newly constructed T3SS vectors were introduced into host strain 8879 (⌬phoP233 ⌬sptP1033::xylE ⌬asdA16), an attenuated derivative of the highly virulent UK-1 strain. The SopE-SO7 fusion protein was secreted by the T3SS of Salmonella. The vector pYA4184 was constructed for delivery of the SO7 antigen via the T2SS. The SO7 protein was toxic to Salmonella when larger amounts were synthesized; thus, the synthesis of this protein was placed under the control of the lacI repressor gene, whose expression in turn was dependent on the amount of available arabinose in the medium. The pYA4184 vector was introduced into host strain 9242 (⌬phoP233 ⌬asdA16 ⌬araBAD23 ⌬relA198::araC P BAD lacI TT [TT is the T4ipIII transcription terminator]). In addition to SO7, for immunization and challenge studies we used the EAMZ250 antigen of Eimeria acervulina, which was previously shown to confer partial protection against E. acervulina challenge when it was delivered via the T3SS. Immunization of chickens with a combination of the SO7 and EAMZ250 antigens delivered via the T3SS induced superior protection against challenge by E. acervulina. In contrast, chickens immunized with SO7 that was delivered via the T2SS of Salmonella were better protected from challenge by E. tenella.

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Immunogenicity of Recombinant Attenuated Salmonella enterica Serovar Typhimurium Vaccine Strains Carrying a Gene That Encodes Eimeria tenella Antigen SO7

Konjufca¹,

Jenkins

Wang³

et al. 2008

Infect Immun

View full text Add to dashboard Cite

show abstract

“…Vaccination of chickens with a ∆ cya crp mutant of S. Typhimurium expressing the E. coli O78 LPS O-antigens induced antibodies against the O78 LPS O-antigen and against Salmonella, and engendered a degree of protection against challenge with a pathogenic E. coli O78 strain (Roland et al, 1999). S. Typhimurium vaccine strains were used as antigen delivery system for oral immunisation of chickens against two antigens of the coccidian parasite Eimeria tenella (Pogonka et al, 2003). However, the delivery of antigens to the immune system is not sufficient per se to engender a protective response.…”

Section: Vaccines and Adaptive Immunitymentioning

confidence: 99%

Salmonella infections: immune and non-immune protection with vaccines

Barrow

2007

Avian Pathology

137

View full text Add to dashboard Cite

“…Yet these strains are very costly to produce, have a limited shelf life, and have the potential to revert to the pathogenic wild type (42). Thus, construction of recombinant vaccines has become an attractive and promising approach to control this disease.…”

mentioning

confidence: 99%

A Recombinant Attenuated Salmonella enterica Serovar Typhimurium Vaccine Encoding Eimeria acervulina Antigen Offers Protection against E. acervulina Challenge

et al. 2006

View full text Add to dashboard Cite

Coccidiosis is a ubiquitous disease caused by intestinal protozoan parasites belonging to several distinct species of the genus Eimeria. Cell-mediated immunity (CMI) is critically important for protection against Eimeria; thus, our approach utilizes the bacterial type III secretion system (TTSS) to deliver an antigen directly into the cell cytoplasm of the immunized host and into the major histocompatibility complex class I antigenprocessing pathway for induction of CMI and antigen-specific cytotoxic T-lymphocyte responses in particular. To accomplish this goal, Eimeria genes encoding the sporozoite antigen EASZ240 and the merozoite antigen EAMZ250 were fused to the Salmonella enterica serovar Typhimurium effector protein gene sptP in the parental pYA3653 vector, yielding pYA3657 and pYA3658, respectively. SptP protein is secreted by the TTSS of Salmonella and translocated into the cytoplasm of immunized host cells. The host strain chromosomal copy of the sptP gene was deleted and replaced by a reporter gene, xylE. The newly constructed vectors pYA3657 and pYA3658 were introduced into host strain 8879 (⌬phoP233 ⌬sptP1033::xylE⌬ asdA16). This strain is an attenuated derivative of the highly virulent strain UK-1. When strain 8879(pYA3653) as the vector control and strain 8879 harboring pYA3657 or pYA3658 were used to orally immunize day-of-hatch chicks, colonization of the bursa, spleen, and liver was observed, with peak titers 6 to 9 days postimmunization. In vitro experiments show that the EASZ240 antigen is secreted into the culture supernatant via the TTSS and that it is delivered into the cytoplasm of Int-407 cells by the TTSS. In vivo experiments indicate that both humoral and cell-mediated immune responses are induced in chickens vaccinated with a recombinant attenuated Salmonella serovar Typhimurium vaccine, which leads to significant protection against Eimeria challenge.

show abstract

A single dose of recombinant Salmonella typhimurium induces specific humoral immune responses against heterologous Eimeria tenella antigens in chicken

Cited by 50 publications

References 43 publications

Immunogenicity of Recombinant Attenuated Salmonella enterica Serovar Typhimurium Vaccine Strains Carrying a Gene That Encodes Eimeria tenella Antigen SO7

Immunogenicity of Recombinant Attenuated Salmonella enterica Serovar Typhimurium Vaccine Strains Carrying a Gene That Encodes Eimeria tenella Antigen SO7

Salmonella infections: immune and non-immune protection with vaccines

A Recombinant Attenuated Salmonella enterica Serovar Typhimurium Vaccine Encoding Eimeria acervulina Antigen Offers Protection against E. acervulina Challenge

Contact Info

Product

Resources

About