A single-electrode electrochemical system for multiplex electrochemiluminescence analysis based on a resistance induced potential difference

Gao, Wenyue; Muzyka, Kateryna; Ma, Xiangui; Lou, Baohua; Xu, Guobao

doi:10.1039/c8sc00410b

Cited by 91 publications

(76 citation statements)

References 34 publications

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“…The ECL chip was a low‐cost single electrode system based on prior work . Briefly, a plastic plate was painted with a commercial conductive carbon ink coating (Jelcon CH‐8, Jujo Chemical Co., LTD, Japan) and covered with an adhesive paper into which holes with 5 mm diameters had been cut, creating wells for detection.…”

Section: Methodsmentioning

confidence: 99%

Improving the limit of detection in portable luminescent assay readers through smart optical design

Wang

et al. 2019

Journal of Biophotonics

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Critical biomarkers of disease are increasingly being detected by point‐of‐care assays. Chemiluminescence (CL) and electrochemiluminescence (ECL) are often used in such assays due to their convenience and that they do not require light sources or other components that could complicate or add cost to the system. Reports of these assays often include readers built on a cellphone platform or constructed from low‐cost components. However, the impact the optical design has on the limit of detection (LOD) in these systems remains unexamined. Here, we report a theoretical rubric to evaluate different optical designs in terms of maximizing the use of photons emitted from a CL or ECL assay to improve the LOD. We demonstrate that the majority of cellphone designs reported in the literature are not optimized, in part due to misunderstandings of the optical tradeoffs in collection systems, and in part due to limitations imposed on the designs arising from the use of a mobile phone with a very small lens aperture. Based on the theoretical rubric, we design a new portable reader built using off‐the‐shelf condenser optics, and demonstrate a nearly 10× performance enhancement compared to prior reports on an ECL assays running on a portable chip.

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Section: Methodsmentioning

confidence: 99%

Improving the limit of detection in portable luminescent assay readers through smart optical design

Wang

et al. 2019

Journal of Biophotonics

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“…Electrochemiluminescence (ECL) is a process in which light is emitted from excited species electrogenerated at the electrode surface during an exergonic electron transfer reaction 31–34. ECL does not need an external light source, and has received considerable attention and extensive application due to its free light source, accurate potential control, simple operation processes and high sensitivity 35–39. When coupled with the immunoassay or molecular imprinting technique, the use of ECL sensors has made great strides in the sensitive and selective detection of diverse analytes including protein, DNA, and other target molecules 40–43.…”

Section: Introductionmentioning

confidence: 99%

Construction of a flexible electrochemiluminescence platform for sweat detection

Chen

Cheng

Ji³

et al. 2019

Chem. Sci.

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“…A SEES differs from a BPE because the driving potential is applied to a single resistive electrode (such as a thin film of ITO), causing a potential drop along its length, whereas, in a bipolar electrochemical system, the driving potential is applied to the electrolyte solution.Despite this difference, the SEES is similar to bipolar electrochemistry in three ways: 1) paired faradaic reactions simultaneously occur on each pole of the electrode, 2) the electrode can be multiplexed without the use of additional leads, and 3) an ECL reporting reaction is used to transduce the current of the sensing reaction. Notably, a SEES can be divided into an array of independent bipolar patches by placing a gasket with an array of openings over the ITO slide 75. Each microelectrochemical cell acts independently because current from each anode will flow only to the nearest cathode.…”

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confidence: 99%

Recent Advancements in Bipolar Electrochemical Methods of Analysis

Rahn

Anand

2020

Anal. Chem.

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The goal of this review is to provide an overview of the advancements made in the field of bipolar electrochemistry over the past 2 years, with an emphasis on analysis. Bipolar electrodes (BPEs) are versatile, and in electroanalysis, they have been used extensively to screen electrocatalysts(1−4) and to sense biomarkers.(5−10) Their ability to modulate local electric fields lends them to the manipulation of cells and to the enrichment and separation of analytes.(11−17) Finally, by virtue of the polar and often graded profile of the interfacial potential across BPEs, they provide a platform for synthesis of Janus particles, useful as sensors and as microswimmers(18−22) and other materials with compositional gradients.(23,24) BPEs are particularly well-suited to analytical challenges that demand multiplexing or amenability to point-of-need (PON) application because even large arrays of BPEs can be controlled with simple equipment yet yield quantitative information about a system. In this review, we discuss recent progress in reactions that transduce current to a visible signal, sensing mechanisms, bipolar electrochemical cell design, integration of bipolar electrochemistry with spectroscopic techniques, BPEs at the nanoscale, and the application of BPEs to electrokinetics and materials preparation. Throughout the discussion, we identify promising trends, innovative directions, and remaining challenges in the field. Disciplines Disciplines Analytical Chemistry Comments Comments

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A single-electrode electrochemical system for multiplex electrochemiluminescence analysis based on a resistance induced potential difference

Abstract: A single-electrode electrochemical system uses only one electrode for multiplex experiments, and is a highly cheap platform for high throughput analysis.

Cited by 91 publications

References 34 publications

Improving the limit of detection in portable luminescent assay readers through smart optical design

Improving the limit of detection in portable luminescent assay readers through smart optical design

Construction of a flexible electrochemiluminescence platform for sweat detection

Recent Advancements in Bipolar Electrochemical Methods of Analysis

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