A single-molecule study of polycrystalline microstructure by fluorescence polarization spectroscopy

Latychevskaia, Tatiana; Renn, Alois; Wild, Urs P.

doi:10.1016/j.jlumin.2005.08.008

Cited by 12 publications

(5 citation statements)

References 32 publications

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“…In this work, we offer an alternative, polarization modulation spectroscopy (PMS), that does not require fast electronics to estimate the number of randomly orientated fluorophores. [7][8][9][10] We have previously demonstrated that the conformation of fluorescent polymers can be determined by PMS. [11][12][13] This is because the depth of the fluorescence intensity modulation is sensitive to the dipole orientation distribution of the fluorophores in the polymers.…”

Section: ' Introductionmentioning

confidence: 99%

“…The experiment is technically challenging. In this work, we offer an alternative, polarization modulation spectroscopy (PMS), that does not require fast electronics to estimate the number of randomly orientated fluorophores. − We have previously demonstrated that the conformation of fluorescent polymers can be determined by PMS. − This is because the depth of the fluorescence intensity modulation is sensitive to the dipole orientation distribution of the fluorophores in the polymers . It suggests that the technique may be applicable to measure rapidly the average number of the fluorophores in individual nanoparticles, if the heterogeneity in dipole orientation is properly taken into account and the probability distribution of the effective fluorophore numbers is adequately simulated with Monte Carlo calculations.…”

Section: Introductionmentioning

confidence: 99%

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Polarization Modulation Spectroscopy of Single Fluorescent Nanodiamonds with Multiple Nitrogen Vacancy Centers

et al. 2011

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A technique based on polarization modulation spectroscopy (PMS) has been developed to determine quantitatively the number of fluorophores in nanoparticles at the single-molecule level. The technique involves rotation of the polarization of the excitation laser on a millisecond time scale, leading to fluorescence intensity modulation. By taking account of the heterogeneous orientation among the dipoles of the fluorophores and simulating the modulation depth distribution with Monte Carlo calculations, we show that it is possible to deduce the ensemble average and number distribution of the fluorophores. We apply the technique to fluorescent nanodiamonds (FNDs) containing multiple nitrogen vacancy (NV) centers. Comparing the experimental and simulated modulation depth distributions of 11 nm FNDs, we deduce an average number of = 3, which is in good agreement with independent photon correlation measurements. The method is general, rapid, and applicable to other nanoparticles, polymers, and molecular complexes containing multiple and randomly orientated fluorophores as well.

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Section: ' Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Polarization Modulation Spectroscopy of Single Fluorescent Nanodiamonds with Multiple Nitrogen Vacancy Centers

et al. 2011

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“…There were several other papers regarding the dynamics of single molecules using fluorescence anisotropy. Latychevskaia et al have reported a single molecule study of polycrystalline microstructure of n-hexadecane at a temperature of 1.7 K, revealing a chromophore-two level system in spatially unresolved molecules (298). Another solid state study was reported (299) by Forster et al, where fluctuations of single conjugated polymer molecules were investigated in a polystyrene matrix at room temperature.…”

Section: Fluorescence Polarization Molecular Dynamics and Related Phe...mentioning

confidence: 99%

Molecular Fluorescence, Phosphorescence, and Chemiluminescence Spectrometry

et al. 2008

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This review covers the approximately two-year period since our last review (A1), roughly from January 2004 through December 2005. A computer search of Chemical Abstracts provided most of the references for this review. Other citations were found through individual searches by the various authors who wrote a particular section of this review. In an effort to more effectively accomplish this goal, we have included authors who are experts in the various subtopics of this review. Coverage is limited to articles that describe new developments in the theory and practice of molecular luminescence for chemical analysis in the ultraviolet, visible, and near-infrared region.Citations may be duplicated between sections due to articles with contents that span several topics. However, in an effort to reduce the length of this review, we have attempted to limit this kind of duplication. In general, citations are limited to journal articles and usually do not include patents, proceedings, reports, and dissertations.We have tried to focus on important advances of general interest and relevance to the field of analytical chemistry, rather than extensions of previous advances. This was done in an effort to continue our recent attempts to significantly reduce the length of this biennial review. In addition, we have also expanded our description of individual citations for better clarification of content where necessary.Although we are not able to provide extensive coverage of developments of relevance to broad areas such as chromatography and biological sciences, we have tried to include major review articles and chapters relevant to these topics. If you feel that we omitted an important article published during the above referenced time period, please forward the reference to one of us and we will be certain to consider it for the next review.

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“…[34] The color-coded distribution in Figure 2 are strongly correlated with the crooked features, and are significantly anti-correlated with each other (compare Figure 4 b, c). The molecules at higher wavelengths than B 3 (16 800-17 000 cm À1 ; Figure 4 c) appear mainly close to the crooked features, whereas the molecules that absorb near the center of A 2 (17 330-17 345 cm À1 ; Figure 4 b) are located in the areas between the crooked regions.…”

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confidence: 92%

“…Synchronous measurements of the spectra and the spatial coordinates of large numbers of SMS provides deeper insights into the nature of structural features of the sample. [34] The color-coded distribution in Figure 2 c reveals a pronounced correlation between the location of SMs in the sample and the spectral position of their ZPLs (see also the movie in the Supporting Information). Other spectral parameters (e.g., the linewidth) may be chosen as well.…”

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confidence: 93%

Far‐Field Nanodiagnostics of Solids with Visible Light by Spectrally Selective Imaging

et al. 2009

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A single-molecule study of polycrystalline microstructure by fluorescence polarization spectroscopy

Cited by 12 publications

References 32 publications

Polarization Modulation Spectroscopy of Single Fluorescent Nanodiamonds with Multiple Nitrogen Vacancy Centers

Polarization Modulation Spectroscopy of Single Fluorescent Nanodiamonds with Multiple Nitrogen Vacancy Centers

Molecular Fluorescence, Phosphorescence, and Chemiluminescence Spectrometry

Far‐Field Nanodiagnostics of Solids with Visible Light by Spectrally Selective Imaging

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