A single-step solid phase extraction for the simultaneous determination of 8 mycotoxins in fruits by ultra-high performance liquid chromatography tandem mass spectrometry

Wang, Meng; Jiang, Nan; Xian, Hong; Wei, Dizhe; Shi, Lei; Feng, Xiaoyuan

doi:10.1016/j.chroma.2015.12.004

Cited by 117 publications

(85 citation statements)

References 29 publications

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“…Owing to the different physical and chemical properties of all the prevalent mycotoxins in fruits, the determination of trace amounts of all them in food represents an extremely challenging task [100]. …”

Section: Prevalence Of Alternaria Mycotoxinsmentioning

confidence: 99%

AlternariaMycotoxins in Food and Feed: An Overview

Escrivá

Oueslati

Font

et al. 2017

Journal of Food Quality

146

127

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Alternaria is one of the major mycotoxigenic fungal genera with more than 70 reported metabolites. Alternaria mycotoxins showed notably toxicity, such as mutagenicity, carcinogenicity, induction of DNA strand break, sphingolipid metabolism disruption, or inhibition of enzymes activity and photophosphorylation. This review reports on the toxicity, stability, metabolism, current analytical methods, and prevalence of Alternaria mycotoxins in food and feed through the most recent published research. Half of the publications were focused on fruits, vegetables, and derived products-mainly tomato and apples-while cereals and cereal byproducts represented 38%. The most studied compounds were alternariol, alternariol methyl ether, tentoxin, and tenuazonic acid, but altenuene, altertoxins (I, II, and III), and macrosporin have been gaining importance in recent years. Solid-liquid extraction (50%) with acetonitrile or ethyl acetate was the most common extraction methodology, followed by QuEChERS and dilutiondirect injection (both 14%). High-and ultraperformance liquid chromatography coupled with tandem mass spectrometry was the predominant determination technique (80%). The highest levels of alternariol and alternariol methyl ether were found in lentils, oilseeds, tomatoes, carrots, juices, wines, and cereals. Tenuazonic acid highest levels were detected in cereals followed by beer, while alternariol, alternariol methyl ether, tenuazonic acid, and tentoxin were found in legumes, nuts, and oilseeds.

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Section: Prevalence Of Alternaria Mycotoxinsmentioning

confidence: 99%

AlternariaMycotoxins in Food and Feed: An Overview

Escrivá

Oueslati

Font

et al. 2017

Journal of Food Quality

146

127

View full text Add to dashboard Cite

show abstract

“…showed that a combination of MCX and NH 2 was the most effective cleanup for patulin and seven other mycotoxins (Wang, Jiang, Xian, Wei, Shi, & Feng, 2016). In-tube solid-phase microextraction (IT-SPME) was coupled with LC-MS for fruit juice and dried fruit samples by Kataoka, Itano, Ishizaki, and Saito (2009) A Carboxen 1006 PLOT capillary column was used as an extraction device.…”

Section: Lc-ms Methodsmentioning

confidence: 99%

Determination of trace patulin in apple-based food matrices

Zhang

2017

Food Chemistry

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“…The sample was extracted according to Wang et al [29]. Orange and cherry fruits were purchased from local markets.…”

Section: Methodsmentioning

confidence: 99%

Development of an Immunochromatographic Strip Test for the Rapid Detection of Alternariol Monomethyl Ether in Fruit

Man

Liang

Jia

et al. 2017

Toxins

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A rapid, portable, and semi-quantitative immunochromatographic strip was developed for rapid and visual detection of alternariol monomethyl ether (AME). For this purpose, the anti-AME monoclonal antibody (mAb) was prepared and identified. AME coupled to bovine serum albumin (BSA) via methyl 4-bromobutanoate was prepared as immunogen. The recoveries of AME in spiked cherry and orange fruits determined by competitive ELISA were 86.1% and 80.7%, respectively. A colloidal gold nanoparticle (CGN) and CGNs-mAb conjugate were synthesized, and on this basis, a competitive colloidal gold immunochromatographic strip was developed and applied to the detection of AME toxin in fruit samples. The intensity of red density of the test line (T line) is inversely proportional to AME concentration in the range 0.1–10 ng/mL. The visual limit of detection (LOD) of AME was found to be about 10 ng/mL. The semi-quantitative detection can be completed in 10 min. Moreover, the immunochromatographic strip has lower cross-reactivity with AME analogues, and it has a good stability performance (following 3 months of storage). Hence, the colloidal gold immunochromatographic strip could be used as a semi-quantitative tool for the on-site, rapid, and visual detection of AME in fruit.

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A single-step solid phase extraction for the simultaneous determination of 8 mycotoxins in fruits by ultra-high performance liquid chromatography tandem mass spectrometry

Cited by 117 publications

References 29 publications

AlternariaMycotoxins in Food and Feed: An Overview

AlternariaMycotoxins in Food and Feed: An Overview

Determination of trace patulin in apple-based food matrices

Development of an Immunochromatographic Strip Test for the Rapid Detection of Alternariol Monomethyl Ether in Fruit

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