A single-tube RT-PCR for rapid detection and differentiation of some African isolates of palyam serogroup orbiviruses

Aradaib, Imadeldin E.; Mohamed, Mohamed E. H.; Abdalla, M. A.

doi:10.1016/j.jviromet.2009.05.019

Cited by 6 publications

(5 citation statements)

References 21 publications

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“…A single-tube RT-PCR assay was carried out for CCHFV RNAs amplification basically as described previously [ 17 ]. Briefly, a standard 50 μl reaction mixture contained in final concentration of 1 × enzyme mix reaction buffer, 5.0 μl of 10 mM dNTP mix, 5.0 μl of 25 mM Mg Cl 2 , 5.0 U enzyme mix, 2.0 μl of 20 picomole of each primers (CCHF1 and CCHF2), 5.0 μl of target RNA, were used.…”

Section: Methodsmentioning

confidence: 99%

A nosocomial transmission of crimean-congo hemorrhagic fever to an attending physician in north kordufan, Sudan

Elata

Karsany²,

Elageb³

et al. 2011

Virol J

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BackgroundCrimean-Congo hemorrhagic fever (CCHF), a tick-borne disease caused by Crimean-Congo hemorrhagic fever virus (CCHFV), is a member of the genus Nairovirus in the family Bunyaviridae. Recently, CCHFV has been reported as an important emerging infectious viral pathogen in Sudan. Sporadic cases and multiple CCHF outbreaks, associated with nosocomial chain of transmission, have been reported in the Kordufan region of Sudan.AimsTo confirm CCHF in an index patient and attending physician in North Kordufan region, Sudan, and to provide some information on virus genetic lineages.MethodsAntibody captured ELISA, reverse transcription PCR, partial S segment sequences of the virus and subsequent phylogenetic analysis were used to confirm the CCHFV infection and to determine the virus genetic lineages.ResultsCCHF was confirmed by monitoring specific IgM antibody and by detection of the viral genome using RT-PCR. Treatment with oral ribavirin, replacement with fluid therapy, blood transfusion and administration of platelets concentrate resulted in rapid improvement of the health condition of the female physician. Phylogenetic analysis of the partial S segment sequences of the 2 CCHFV indicates that both strains are identical and belong to Group III virus lineage, which includes viruses from Africa including, Sudan, Mauritania, South Africa and Nigeria.ConclusionFurther epidemiologic studies including, CCHFV complete genome analysis and implementation of improved surveillance are urgently needed to better predict and respond to CCHF outbreaks in the Kordufan region, Sudan.

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Section: Methodsmentioning

confidence: 99%

A nosocomial transmission of crimean-congo hemorrhagic fever to an attending physician in north kordufan, Sudan

Elata

Karsany²,

Elageb³

et al. 2011

Virol J

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“…Chuzan virus, a member of the Palyam serogroup of orbiviruses in the family Reoviridae, was isolated from the biting midge Culicoides oxytoma and from sentinel calves in 1985 [28,29]. The genome of this virus consists of 10 dsRNA segments that encode seven structural (VP1-VP7) and four non-structural (NS1, NS2, NS3, and NS3A) proteins.…”

Section: Chuzan Virusmentioning

confidence: 99%

“…The genome of this virus consists of 10 dsRNA segments that encode seven structural (VP1-VP7) and four non-structural (NS1, NS2, NS3, and NS3A) proteins. The virus was subsequently implicated in an episoed of congenital abnormalities with the hydranencephaly-cerebellar hypoplasia syndrome among calves in the Kyushu district, from autumn 1985 through spring 1986 [28]. This virus has been associated with various haematophagous arthropod vectors and large mammals, principally cattle, in many parts of the world, including Asia, Australia, and Africa [26,29,30].…”

Section: Chuzan Virusmentioning

confidence: 99%

Infectious Causes of Reproductive Disorders in Cattle

Yoo

2010

Journal of Reproduction and Development

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Abstract. The incidences of reproductive disorders in bovine are increasing over years. This scenario is further aggravating due to more emphasis on selection and rearing of animal for specific commercial purposes which compromises livestock reproduction. Reproductive disorders like infertility and abortions in cattle are major problems in the bovine industry. The reproductive disorders might be caused by several different agents such as physical agents, chemical agents, biological agents, etc. Also, the causative agent and pathogenesis of reproductive disorders are influenced by various factors including environmental factor. The exact causes may not be evident and are often complicated with multiple causative agents. Thus, there is a need for multi-faceted approach to understand correlation of various factors with reproductive performance. Of the agents, infectious biological agents are significant cause of reproductive disorder and are of high priority in the bovine industry. These factors are not only related to the prosperity of bovine industry but are also important from public health point of view because of their zoonotic potentials. Several infectious agents like bacterial, viral, protozoon, chlamydial and fungal agents are known to have direct impact on reproductive health of cattle. These diseases can be arranged and discussed in different groups based on the causative agents.

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“…The Palyam serogroup of the genus Orbivirus and family Reoviridae are arthropod-borne viruses that have been isolated in Asia, Africa, and Australia [1,2]. Some of the viruses are associated with abortion and teratogenesis in cattle and other ruminants [3,4,5,6].…”

Section: Introductionmentioning

confidence: 99%

“…Currently, 13 serotypes are recognized by the International Committee on Taxonomy of Viruses (ICTV): Palyam, Kasba, Vellore, Abadina, D’Aguilar, Nyabira, CSIRO Village, Marrakai, Gweru, Bunyip Creek, Petevo, Marondera and Kindia. The serotypes are defined by the serum neutralisation test, but cross-reactions occur, separating the viruses into six antigenic complexes [1,7]. Although some Palyam serogroup viruses had been isolated previously from aborted foetuses [7,8], it was only after an outbreak of congenital abnormalities in cattle in Japan in 1985–1986 that their pathogenic importance was investigated [6,9].…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic Characterization of the Palyam Serogroup Orbiviruses

Ebersohn

Coetzee

Snyman

et al. 2019

Viruses

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The Palyam serogroup orbiviruses are associated with abortion and teratogenesis in cattle and other ruminants. Of the 13 different serotypes that have been identified, the full genome sequence of only one, Kasba, has been published. We undertook to perform Next Generation Sequencing (NGS) and phylogenetic analysis on 12 Palyam serotypes plus field isolates of the African serotypes in our possession. The Palyam serogroup was found to be most closely related to the African horse sickness virus group and showed the most distant evolutionary relationship to the equine encephalosis viruses (EEV). Amino acid sequence analysis revealed that the gene encoding VP7 was the most conserved within serotypes and VP2 and VP5 showed the highest degree of variation. A high degree of sequence identity was found for isolates from the same geographical region. The phylogenetic analysis revealed two clades where the African serotypes were all very closely related in one clade and the other clade contained the Australian and Asian serotypes and one African serotype, Petevo. It was evident from the sequence data that the geographical origin of Palyam serogroup viruses played an important role in the development of the different serotypes.

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A single-tube RT-PCR for rapid detection and differentiation of some African isolates of palyam serogroup orbiviruses

Cited by 6 publications

References 21 publications

A nosocomial transmission of crimean-congo hemorrhagic fever to an attending physician in north kordufan, Sudan

A nosocomial transmission of crimean-congo hemorrhagic fever to an attending physician in north kordufan, Sudan

Infectious Causes of Reproductive Disorders in Cattle

Phylogenetic Characterization of the Palyam Serogroup Orbiviruses

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