A siphonage flow and thread-based low-cost platform enables quantitative and sensitive assays

Abstract

For pump-free, material abundant, portable, and easy-to-operate low-cost microfluidics, a siphonage flow microfluidic thread-based analytical device (S-μTAD) platform enabling quantitative and sensitive assays was designed. Renewable and continuous siphonage flow allowed replicate sampling and detection on one channel/device, obviating some possible inconsistencies among channels or devices. Y-shaped channels were fabricated with polyester cotton blend thread, due to its greater chemiluminescent sensitivity in comparison with that of cotton and polyester threads. S-μTAD sensors for glucose and uric acid were fabricated by using oxidase-immobilized cotton thread as the sample arm of the channels. The acceptable reproducibility and high sensitivity, demonstrated by the relative standard deviations of less than 5% in all cases and the detection limits of 4 × 10(-8) mol L(-1) for hydrogen peroxide, 1 × 10(-7) mol L(-1) for glucose, and 3 × 10(-6) mol L(-1) for uric acid, demonstrated the feasibility of the S-μTAD for quantitative assays. Good agreements between S-μTAD/sensor results and hospital results for blood glucose and uric acid assays indicated the capability of S-μTAD/sensors for the analysis of real samples. These findings proved the utility of siphonage for low-cost microfluidics and the suitability of our S-μTAD design for quantitative assays.

Surface Modified Thread-Based Microfluidic Analytical Device for Selective Potassium Analysis

“…The precision of the procedure using different devices was calculated at three potassium concentrations (1.26x10 -4 , 1.26x10 -3 , 4.00x10 -2 M) using 10 replicates each, obtaining a mean relative standard deviation of 1.34% (Table 1). This means a better precision compared to other thread-based procedures: Mao et al 28 found a 9.3% RSD for the biomarker squamous cell carcinoma antigen test; the procedure for DNA detection described by of Du et al 29 has a precision of 9.8%; Lu et al 31 found precisions around 5% in their enzymatic test for hydrogen peroxide and related analytes based on chemiluminescence measurement. The precision figures found here can be ascribed to the simple device used and the use of the H coordinate.…”

Thread based electrofluidic platform for direct metabolite analysis in complex samples

“…The second group are based upon single threads, which generally involve much smaller solution volumes, as in these examples the flow within the strands of the thread is confined to one direction. The use of this later platform has been applied to bacteria isolation and quantification [24], chemotaxis studies for cell culture systems [25], immunoassays [26,27], blood typing [28], chemical synthesis [29], and the determination of nucleic acids [30,31], proteins [4,7,29,[31][32][33][34], glucose [29,[35][36][37], drugs [38], small ions [6,8,32,39] and metals [40]. Several detection techniques have been used for these various applications, with the most common, albeit the least sensitive, being simple colorimetric detection.…”

Optimization Design of a Rain-Power Utilization System Based on a Siphon and Its Application in a High-Rise Building

“…Furushima [27] replaced the traditional auxiliary pumping device with a siphon cooling device as a cooling water system of a photovoltaic model, producing an obvious result in energy conservation. Lu [28] introduced continuous siphonage flow as a renewable fluid driving force to improve the detection reproducibility for trace analytes in quantitative and sensitive assays.…”