A small basic protein from the brz-brb operon is involved in regulation of bop transcription in Halobacterium salinarum

Tarasov, Valery; Schwaiger, Rita; Furtwängler, Katarina; Dyall‐Smith, Mike; Oesterhelt, Dieter

doi:10.1186/1471-2199-12-42

Cited by 13 publications

(14 citation statements)

References 32 publications

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“…Furthermore, proteins dealing with ion transport and stress response showed similar response patterns. The light-driven proton pump bacteriorhodopsin (BR), which represents an important mechanism for energy conservation under anaerobic conditions with light [55], showed an 01Down-15UP pattern, with strong induction in mature biofilms. Resembling patterns were observed for Bop (bacterio-opsin as the apo-protein of BR) and connected proteins (e.g.…”

Section: Proteins Decreasing and Increasing During Biofilm Developmenmentioning

confidence: 99%

Shedding light on biofilm formation of Halobacterium salinarum R1 by SWATH‐LC/MS/MS analysis of planktonic and sessile cells

et al. 2016

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Early and mature biofilm formation in the extremely halophilic euryarchaeon Halobacterium salinarum strain R1 was characterized by SWATH-LC/MS/MS. Using a simple surfactant-assisted protein solubilization protocol and one-dimensional ultra-high performance nanoflow chromatography on the front end, 63.2 and 58.6% of the predicted H. salinarum R1 proteome could be detected and quantified, respectively. Analysis of biophysical protein properties, functional analysis and pathway mapping indicated comprehensive characterization of the proteome. Sixty point eight percent of the quantified proteins (or 34.5% of the predicted proteome) exhibited significant abundance changes between planktonic and sessile states, demonstrating that haloarchaeal biofilm formation represents a profound "lifestyle change" on the molecular level. Our results and analysis constitute the first comprehensive study to track molecular changes from planktonic cultures to initial and mature archaeal biofilms on the proteome level. Data are available via ProteomeXchange, identifier PXD003667. Proteins exemplifying different protein expression level profiles were selected, and their corresponding gene transcripts targeted by qRT-PCR to test the feasibility of establishing rapid PCR-based assays for archaeal biofilm formation.

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Section: Proteins Decreasing and Increasing During Biofilm Developmenmentioning

confidence: 99%

Shedding light on biofilm formation of Halobacterium salinarum R1 by SWATH‐LC/MS/MS analysis of planktonic and sessile cells

et al. 2016

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“…Most strikingly of the gene expression profile, was the very low expression levels (linear fold change of -19.10 or log 2 ratio of -4.10) of the bop gene (encoding for the bacterio-opsin protein) during stationary phase in the Δ CYP174A1 strain . The bop gene forms part of a cluster of genes, called the bop -gene regulon ( Peck et al, 2001 ; Tarasov et al, 2008 , 2011 ; DasSarma et al, 2012 ) that is involved in the biosynthesis and regulation of BR in PM. Figure 2A illustrates the gene expression profiles of bop and other genes associated with the bop -regulon during both phases of growth as mentioned above.…”

Section: Resultsmentioning

confidence: 99%

“…The bop gene forms part of a tightly regulated cluster of genes referred to as the bop -regulon ( Tarasov et al, 2008 , 2011 ; DasSarma et al, 2012 ). Bop is regulated by a sensor regulator gene called bat and potentially also by a small zinc-finger containing protein called brz.…”

Section: Discussionmentioning

confidence: 99%

Complex Effects of Cytochrome P450 Monooxygenase on Purple Membrane and Bacterioruberin Production in an Extremely Halophilic Archaeon: Genetic, Phenotypic, and Transcriptomic Analyses

et al. 2018

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Halophilic archaea are known to produce a diverse array of pigments for phototrophy and photoprotection. The aim of this paper was to determine the role of a Halobacterium gene encoding the predicted cytochrome P450 monooxygenase (CYP174A1) in pigment synthesis through a combined genetic, phenotypic, and transcriptomic approach. We report on the observed phenotype changes [increased bacterioruberin levels and the loss of purple membrane (PM)] between the Halobacterium salinarum R1 and its CYP174A1-deletion mutant. In addition, we report on the whole-genome DNA microarray analysis, which supports the phenotype of PM loss. This work expands our understanding of the bop-gene regulon, and its relation to carotenoid biosynthesis, and sheds light on our broader understanding of the role (s) of CYP174A1 in archaeal pigment synthesis. To date, this is the first study in which the physiological role of any cytochrome P450 monooxygenase (CYP450) in extremely halophilic archaea has been reported.

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“…mediterranei possess potential zinc recognition sites [85]. Another potential connection between this zinc transporter and carotenogenesis could be the "bop-gene regulon [69,[86][87][88]. bop gene is part of a cluster of genes, which is tightly regulated by a sensor regulator called bat and potentially also by a small zinc-finger containing a protein called Brz [69].…”

Section: Discussionmentioning

confidence: 99%

Deciphering Pathways for Carotenogenesis in Haloarchaea

et al. 2020

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Bacterioruberin and its derivatives have been described as the major carotenoids produced by haloarchaea (halophilic microbes belonging to the Archaea domain). Recently, different works have revealed that some haloarchaea synthetize other carotenoids at very low concentrations, like lycopene, lycopersene, cis- and trans-phytoene, cis- and trans-phytofluene, neo-β-carotene, and neo-α-carotene. However, there is still controversy about the nature of the pathways for carotenogenesis in haloarchaea. During the last decade, the number of haloarchaeal genomes fully sequenced and assembled has increased significantly. Although some of these genomes are not fully annotated, and many others are drafts, this information provides a new approach to exploring the capability of haloarchaea to produce carotenoids. This work conducts a deeply bioinformatic analysis to establish a hypothetical metabolic map connecting all the potential pathways involved in carotenogenesis in haloarchaea. Special interest has been focused on the synthesis of bacterioruberin in members of the Haloferax genus. The main finding is that in almost all the genus analyzed, a functioning alternative mevalonic acid (MVA) pathway provides isopentenyl pyrophosphate (IPP) in haloarchaea. Then, the main branch to synthesized carotenoids proceeds up to lycopene from which β-carotene or bacterioruberin (and its precursors: monoanhydrobacterioriberin, bisanhydrobacterioruberin, dihydrobisanhydrobacteriuberin, isopentenyldehydrorhodopsin, and dihydroisopenthenyldehydrorhodopsin) can be made.

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A small basic protein from the brz-brb operon is involved in regulation of bop transcription in Halobacterium salinarum

Cited by 13 publications

References 32 publications

Shedding light on biofilm formation of Halobacterium salinarum R1 by SWATH‐LC/MS/MS analysis of planktonic and sessile cells

Shedding light on biofilm formation of Halobacterium salinarum R1 by SWATH‐LC/MS/MS analysis of planktonic and sessile cells

Complex Effects of Cytochrome P450 Monooxygenase on Purple Membrane and Bacterioruberin Production in an Extremely Halophilic Archaeon: Genetic, Phenotypic, and Transcriptomic Analyses

Deciphering Pathways for Carotenogenesis in Haloarchaea

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