A small cosmid for efficient cloning of large DNA fragments

Höhn, Barbara; Collins, John

doi:10.1016/0378-1119(80)90069-4

Cited by 816 publications

(314 citation statements)

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“…2 indicates that hybridization is obtained only in fraction I in a 1.1 kb PstI fragment. A cosmid library of fraction I DNA was constructed by ligating BamHI-cleaved pHC79 with partially Sau3A cleaved fraction I DNA as described (13 (29). The 1.1 kb fragment was isolated from the agarose gel and subcloned in both orientations into the M13mp8 vector (18).…”

Section: Methodsmentioning

confidence: 99%

“…Labelling at the 3' end was difficult to perform suggesting strong secondary structure at this end of the RNA molecule. Using conventional RNA sequencing techniques (19) 13 partial sequences of the 7S RNA at the 5' and 3' ends were sufficient, however, to detect start and end for the 7S RNA gene in the sequence of the 850 bp SalI fragment, assuming that the 7S RNA is the direct transcript of the gene. As indicated in Fig.…”

Section: Methodsmentioning

confidence: 99%

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Common structural features of the genes for two stable RNAs fromHalobacterium halobium

Moritz¹,

Lankat–Buttgereit²,

Groß³

et al. 1985

Nucl Acids Res

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The genes coding for the 5S rRNA and another stable RNA, termed 7S RNA, in Halobacterium halobium were isolated from a genomic library of this archaebacterium and their nucleotide sequences determined. Both genes are colinear with their transcripts (5S rRNA and 7S RNA), but 5S rRNA and possibly also 7S RNA isolated from other halobacteria carry additional nucleotides within the RNA transcript. Both genes are located in the G+C rich chromosomal fraction I of H. halobium. Comparison of the 3' non-coding regions of both genes shows a 20 bp sequence of high homology immediately at the 3' ends which is almost symmetrically flanked by two stem-loop structures, one being situated close to the 3' end but within the coding region and the other downstream of the common 20 bp sequence. INTRODUCTIONAs first shown by Woese (1), 16S ribosomal RNAs from archaebacteria differ considerably in their nucleotide sequence from those of normal eubacteria. More recent reports on the sequences of 5S rRNA from various archaebacteria, such as Thermoplasma acidophilum, Halobacterium cutirubrum and Halococcus morrhuae showed also substantial differences in their primary sequences as well as in their putative secondary structures compared to those of eubacteria and eukaryotes. Moreover it was demonstrated that the 5S

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Common structural features of the genes for two stable RNAs fromHalobacterium halobium

Moritz¹,

Lankat–Buttgereit²,

Groß³

et al. 1985

Nucl Acids Res

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“…E. coli K-12 strain HB101 was used as recipient in cloning procedures. Plasmid pHC79 is a cosmid cloning vector [18], and pBR322 was used for subcloning experiments.…”

Section: Bacterial Strains Plasmids and Growth Mediamentioning

confidence: 99%

“…Large 30-kb chromosomal Sau3A fragments were Iigated into the BamHI site of cosmid vector pHC79 and transduced into HB101 with the help of the cosmid packaging system [18], and subcloningwas done into pBR322. Isolation and purification of DNA fragments from agarase gels was performed by electroelution [19].…”

Section: Recombinant Dna Techniques and Enzymesmentioning

confidence: 99%

Molecular cloning of the F8 fimbrial antigen from Escherichia coli

Hacker¹

1986

FEMS Microbiology Letters

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“…The clone RClO-16 was isolated from a human genomic library in the cosmid pHC79 [6] after screening with a pig kidney Na+,K+-ATPase cysubunit hybridization probe [7]. The restriction map of the RClO-16 clone is presented in fig.1.…”

Section: Introductionmentioning

confidence: 99%

Family of human Na⁺,K⁺‐ATPase genes Structure of the putative regulatory region of the α⁺‐gene

Bessarab¹,

Malyshev²,

Petrukhin³

et al. 1989

FEBS Letters

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The primary structure of the putative regulatory region of a gene of the Na+,K+-ATPase multigene family in the human genome has been determined. This region includes the first exon with all of the untranslatable sequence of mRNA and a dozen nucleotides, coding for the first four amino acids of the hypothetic precursor of the a+-subunit. The entire region comprises over 1400 bp. The possible role of specific nucleotide blocks within this region in comparison with other genes is discussed.

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A small cosmid for efficient cloning of large DNA fragments

Cited by 816 publications

References 11 publications

Common structural features of the genes for two stable RNAs fromHalobacterium halobium

Common structural features of the genes for two stable RNAs fromHalobacterium halobium

Molecular cloning of the F8 fimbrial antigen from Escherichia coli

Family of human Na⁺,K⁺‐ATPase genes Structure of the putative regulatory region of the α⁺‐gene

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A small cosmid for efficient cloning of large DNA fragments

Cited by 816 publications

References 11 publications

Common structural features of the genes for two stable RNAs fromHalobacterium halobium

Common structural features of the genes for two stable RNAs fromHalobacterium halobium

Molecular cloning of the F8 fimbrial antigen from Escherichia coli

Family of human Na+,K+‐ATPase genes Structure of the putative regulatory region of the α+‐gene

Contact Info

Product

Resources

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Family of human Na⁺,K⁺‐ATPase genes Structure of the putative regulatory region of the α⁺‐gene