A snake-like serine proteinase (PmSnake) activates prophenoloxidase-activating system in black tiger shrimp Penaeus monodon

Monwan, Warunthorn; Amparyup, Piti; Tassanakajon, Anchalee

doi:10.1016/j.dci.2016.09.016

Cited by 27 publications

(12 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Serpin was the most highly upregulated transcript in CYp-infected insects, whereas the snake-like serine protease was among the most highly downregulated ones. Clip domain serine proteases, such as the snake-like ones, are involved in the activation of the ProPO proteolytic cascade in invertebrate immune systems (31,32), while serpins from different arthropod species inhibit clip domain serine proteases by blocking the activation of ProPO melanization pathway (33,34). The ProPO cascade is involved in melanization and encapsulation processes and provides arthropod immunity to bacteria, fungi, protozoan, and parasites (34).…”

Section: Discussionmentioning

confidence: 99%

Two Phytoplasmas Elicit Different Responses in the Insect Vector Euscelidius variegatus Kirschbaum

et al. 2018

View full text Add to dashboard Cite

Phytoplasmas are plant-pathogenic bacteria transmitted by hemipteran insects. The leafhopper is a natural vector of chrysanthemum yellows phytoplasma (CYp) and a laboratory vector of flavescence dorée phytoplasma (FDp). The two phytoplasmas induce different effects on this species: CYp slightly improves whereas FDp negatively affects insect fitness. To investigate the molecular bases of these different responses, transcriptome sequencing (RNA-seq) analysis of infected with either CYp or FDp was performed. The sequencing provided the first transcriptome assembly for a phytoplasma vector and a starting point for further analyses on differentially regulated genes, mainly related to immune system and energy metabolism. Insect phenoloxidase activity, immunocompetence, and body pigmentation were measured to investigate the immune response, while respiration and movement rates were quantified to confirm the effects on energy metabolism. The activation of the insect immune response upon infection with FDp, which is not naturally transmitted by, confirmed that this bacterium is mostly perceived as a potential pathogen. Conversely, the acquisition of CYp, which is naturally transmitted by , seems to increase the insect fitness by inducing a prompt response to stress. This long-term relationship is likely to improve survival and dispersal of the infected insect, thus enhancing the opportunity of phytoplasma transmission.

show abstract

Section: Discussionmentioning

confidence: 99%

Two Phytoplasmas Elicit Different Responses in the Insect Vector Euscelidius variegatus Kirschbaum

et al. 2018

View full text Add to dashboard Cite

show abstract

“…The PGRPs and βGRPs detect PGNs and β-1,3-glucans, which activate a clip-domain serine protease (CLIP) cascade that converts prophenoloxidase to active phenoloxidase, leading to the melanisation responses involved in eliminating pathogens ( Monwan, Amparyup & Tassanakajon, 2017 ; Li et al, 2016 ). We identified two CLIP genes ( LgSnake-1 and LgSnake-2 ) in the SPB transcriptome datasets.…”

Section: Resultsmentioning

confidence: 99%

RNA interference-mediated silencing of genes involved in the immune responses of the soybean pod borer Leguminivora glycinivorella (Lepidoptera: Olethreutidae)

Ran

Liu

et al. 2018

PeerJ

View full text Add to dashboard Cite

RNA interference (RNAi) technology may be useful for developing new crop protection strategies against the soybean pod borer (SPB; Leguminivora glycinivorella), which is a critical soybean pest in northeastern Asia. Immune-related genes have been recently identified as potential RNAi targets for controlling insects. However, little is known about these genes or mechanisms underlying their expression in the SPB. In this study, we completed a transcriptome-wide analysis of SPB immune-related genes. We identified 41 genes associated with SPB microbial recognition proteins, immune-related effectors or signalling molecules in immune response pathways (e.g., Toll and immune deficiency pathways). Eleven of these genes were selected for a double-stranded RNA artificial feeding assay. The down-regulated expression levels of LgToll-5-1a and LgPGRP-LB2a resulted in relatively high larval mortality rates and abnormal development. Our data represent a comprehensive genetic resource for immune-related SPB genes, and may contribute to the elucidation of the mechanism regulating innate immunity in Lepidoptera species. Furthermore, two immune-related SPB genes were identified as potential RNAi targets, which may be used in the development of RNAi-mediated SPB control methods.

show abstract

“…Other cSP genes, SNK (homologue of Psh) and EA (homologue of SPE) are involved in embryonic development, which could interact with GD and Nudel to form a proteolytic cascade, culminating with proteolytic activation of SPZ and mediated the Toll pathway [59,60]. A recent study in shrimp Penaeus monodon show that the SNK-like could also activate the proPO system, which mediated the melanization in immune response [61], showing that the developmental-related cSP may also participate in immune response in different species. In our results, the SNK, EA, and GD were expanded in T. armoricanus genome, and multiple genes of SNK, EA, and GD were up-regulated after P. hepiali or O. sinensis infection, implying the proteolytic cascade formed by EA, SNK, GD, and Nudel might have multiple function in T. armoricanus.…”

Section: Common Responses In T Armoricanus After P Hepiali or O Simentioning

confidence: 99%

Comparative Transcriptome Analysis of Thitarodes Armoricanus in Response to the Entomopathogenic Fungi Paecilomyces Hepiali and Ophiocordyceps Sinensis

Rao¹,

Cao²,

Wu³

et al. 2019

Insects

View full text Add to dashboard Cite

Thitarodes armoricanus is a medicinal and economically important Lepidopteran insect species. The larvae infected by Paecilomyces hepiali survive no more than four days, while those infected by Ophiocordyceps sinensis can survive for several months before mummification. This provides a valuable comparative system to study interactions between an insect host and different pathogenic fungi. By using the T. armoricanus genome, a time-course transcriptome analysis of the whole larvae without guts was performed to explore the larvae response to P. hepiali and O. sinensis infection. A total of 3106 differentially expressed genes in five clusters were identified. The genes involved in coagulation and multiple metabolisms were both suppressed after P. hepiali or O. sinensis infection, whereas those related to environmental information responses, cell processes, biotic stimulus, and immunity (such as cecropin (CEC)) were elevated. The rapid death of T. armoricanus after P. hepiali infection might be caused by osmotic imbalance, immunocompromise (such as DEFs and GLVs), and nervous system dysfunction (glutamatergic synapse). Up-regulation of the genes related to cuticle structure, nervous system (such as neurotrophin signal pathway and dopaminergic synapse) and immune effectors (such as attacin (ATT) and proline-rich antimicrobial peptide 1 (PRAMP1)) in T. armoricanus, may contribute to the co-existence of T. armoricanus and O. sinensis. This study provides a global view and potential key genes of the interaction between T. armoricanus and two fungal entomopathogens.

show abstract

A snake-like serine proteinase (PmSnake) activates prophenoloxidase-activating system in black tiger shrimp Penaeus monodon

Cited by 27 publications

References 37 publications

Two Phytoplasmas Elicit Different Responses in the Insect Vector Euscelidius variegatus Kirschbaum

Two Phytoplasmas Elicit Different Responses in the Insect Vector Euscelidius variegatus Kirschbaum

RNA interference-mediated silencing of genes involved in the immune responses of the soybean pod borer Leguminivora glycinivorella (Lepidoptera: Olethreutidae)

Comparative Transcriptome Analysis of Thitarodes Armoricanus in Response to the Entomopathogenic Fungi Paecilomyces Hepiali and Ophiocordyceps Sinensis

Contact Info

Product

Resources

About