A SNP panel for early detection of artificial chimerism in HSCT patients using TaqMan technology

Sathirapatya, Tikumphorn; Worrapitirungsi, Wikanda; Sukawutthiya, Poonyapat; Rasmeepaisarn, Kawin; Vongpaisarnsin, Kornkiat

doi:10.1007/s00414-020-02276-2

Cited by 5 publications

(3 citation statements)

References 22 publications

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“…We improved the detection method to serve the detection of donor microchimerism post MST. In previous study, Tikumphorn Sathirapatya and colleagues [ 11 ] used 13 SNPs markers from a TaqMan® Predesigned SNP Genotyping Assay (Applied Biosystems) to provide genotypes from allelic discrimination plot. Their SNP panel could effectively detect 90% of recipient-donor pairs.…”

Section: Discussionmentioning

confidence: 99%

“…The strengths of this method are its higher sensitivity (0.5–1%) and better quantitative accuracy than what is achievable with most PCR-based assays. Recently, real-time PCR (qPCR), droplet digital PCR (ddPCR) based on STR [ 10 ] or next-generation sequencing (NGS) based on single nucleotide polymorphism (SNP) [ 11 , 12 ] have been evaluated with higher sensitivity and more accurate quantification. These novel methods are suitable for assessment of chimerism in the micro range (< 1%).…”

Section: Introductionmentioning

confidence: 99%

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The clinical application of SNP-based next-generation sequencing (SNP-NGS) for evaluation of chimerism and microchimerism after HLA-mismatched stem cell microtransplantation

Feng

et al. 2022

Int J Hematol

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Genetic diagnostic methods for evaluation of chimerism after HSCT, such as STR-PCR and XY-FISH, have limited sensitivity. When donor chimerism is in the micro range (< 1%), deviations in the accuracy of assessment are the most significant disadvantage of these common methods. We developed a highly sensitive method that applies SNPs based on NGS in order to explore the value of donor cell microchimerism in microtransplantation (MST). This improved SNP-NGS approach has higher sensitivity (0.01–0.05%) and only requires a small amount of DNA (8–200 ng). We retrospectively analyzed the clinical data of 48 patients with AML who received HLA-mismatched stem cell MST at our center to assess the impact of microchimerism on clinical prognosis. Patients whose duration of microchimerism was > 10.5 months (median) had a relapse rate of 26.1%, and had better 5-year LFS and OS (73.4% and 82.6%). In contrast, patients whose duration of microchimerism was < 10.5 months had a higher relapse rate (69.6%), and their 5-year LFS and OS were 30.4% and 43.5%. In conclusion, duration of donor chimerism is highly valuable for assessment of survival and prognosis in patients with AML who have received HLA-mismatched stem cell MST, especially the intermediate-risk group.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The clinical application of SNP-based next-generation sequencing (SNP-NGS) for evaluation of chimerism and microchimerism after HLA-mismatched stem cell microtransplantation

Feng

et al. 2022

Int J Hematol

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“…The STR markers are used to monitor chimerism status in hematopoietic stem cell transplantation (HSCT), an essential process for transplant rejection. Also, it monitors the chimerism percentage and stability of STR markers in leukemic patients after transplantation [50][51][52].…”

Section: Introductionmentioning

confidence: 99%

The Impact of Leukemia on the Detection of Short Tandem Repeat (STR) Markers

Alharbi¹,

Alamri²,

El-Shehawi³

2022

Cureus

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Introduction: Short tandem repeats (STRs) have been used for various identity typing methods worldwide. They have high discrimination power in human identification in forensics, paternity testing, missed personal identification, genetic diseases, and gene regulatory functions. They have also been used to detect and monitor the stability of diseases, including various types of cancer. This study aimed to investigate the impact of leukemia on the detection and stability of STR markers.Methods: DNA was isolated from 30 participants (15 with chronic myeloid leukemia( CML) and 15 healthy controls) and used to amplify STR markers using specific primers.Results: We found that the blood of those with leukemia had more 9.3 and 9 alleles at the tyrosine hydroxylase 1 (TH01) marker than the blood of the healthy control samples. The results of this study will help researchers understand leukemia's effect on the detection and stability of STR markers in leukemic patients compared to healthy individuals. Conclusion:Our results demonstrate that STR markers could become useful in genetic studies of leukemia cases.

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Can novel methods replace the gold standard chimerism method after allogeneic hematopoietic stem cell transplantation?

Sel,

Oğuz

2023

Ann Hematol

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A SNP panel for early detection of artificial chimerism in HSCT patients using TaqMan technology

Cited by 5 publications

References 22 publications

The clinical application of SNP-based next-generation sequencing (SNP-NGS) for evaluation of chimerism and microchimerism after HLA-mismatched stem cell microtransplantation

The clinical application of SNP-based next-generation sequencing (SNP-NGS) for evaluation of chimerism and microchimerism after HLA-mismatched stem cell microtransplantation

The Impact of Leukemia on the Detection of Short Tandem Repeat (STR) Markers

Can novel methods replace the gold standard chimerism method after allogeneic hematopoietic stem cell transplantation?

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