A Species Comparison of Serum Proteins and Enzymes by Starch Gel Electrophoresis.

Lawrence, S.H.; Melnick, P. J.; Weimer, Henry E.

doi:10.3181/00379727-105-26180

Cited by 122 publications

(22 citation statements)

References 1 publication

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“…For each enzyme two replicate electrophoretic separations were carried out. After electrophoresis, gels were immediately stained in enzyme-specific reaction mixtures (Table 2) (Lawrence, Melnick & Weimer, 1960;Berjaud & d'Auzac, 1986;, Pasteur et al, 1987;see Keller, 1992). The relative electrophoretic mobilities (R,) of the isozyme bands were calculated according to the BPB dye front and the distance of migration of bands observed on fresh gels.…”

Section: Electrophoresis and Isozyme Stainingmentioning

confidence: 99%

Isozyme variation and somatic incompatibility in populations of the ectomycorrhizal fungus Suillus collinitus

1996

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S0MM.4RYIsozyme variation and somatic incompatibility were investigated in order to study intraspecific genetic variation and to identify genets in natural populations of the ectomycorrhizal fungus Suillus collinitus (Fr.) O. Kuntze, Forty-three isolates, obtained from basidiocarps collected in 11 forest sites under Pinus trees, were examined. Isozyme analysis indicated high intraspecific variation between isolates from the same location and from different locations. Phenetic analysis based on calculated dissimilarity values derived from isozyme banding patterns separated the isolates into two main groups (I and II) with 35 % dissimilarity. A specific and highly active acid phosphatase isoform (ACP-2) was detected in most group II isolates. Dikaryotic isolates displaying isozyme similarity coefficient of 100 % were found to be somatically compatible and were considered to originate from the same genet. The same genet was never found at more than two forest sites. This study showed a high correlation between the results of electrophoretic isozyme analysis and those of somatic incompatibility reactions. The results were discussed in terms of population biology.

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Section: Electrophoresis and Isozyme Stainingmentioning

confidence: 99%

Isozyme variation and somatic incompatibility in populations of the ectomycorrhizal fungus Suillus collinitus

1996

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“…Esterases were stained on the gel [17,18] using the following specific substrates: a-naphthyl acetate, ,-naphthyl acetate, a-naphthyl propionate, /,-naphthyl propionate, a-naphthyl butyrate, fl-naphthyl butyrate and indoxyl acetate (Sigma, St Louis, Missouri, U.S.A.). Sensitivity of esterases to diisopropylfluorophosphate (DFP) (10-3 M) was also tested.…”

Section: Esterase Electrophoresismentioning

confidence: 99%

Complexity ofPseudomonas aeruginosainfection in cystic fibrosis: combined results from esterase electrophoresis and rDNA restriction fragment length polymorphism analysis

Denamur

Picard²,

Goullet³

et al. 1991

Epidemiol. Infect.

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SUMMARYEsterase electrophoretic typing and restriction fragment length polymorphism of ribosomal DNA regions (ribotyping) were used to differentiate 102 Pseudomonas aeruginosa clinical isolates obtained from chronic lung infection in 23 patients with cystic fibrosis (CF) and two reference strains (including the type strain ATCC 10145). Twenty-five zymotypes were obtained with the former method and 16 ribotypes with the latter. Combination of the two typing systems led to the finding of 30 different types. Our data highlights the physiopathological complexity of P. aeruginosa infection in CF as, in six individual cases, several types were found among isolates from a given patient. On the other hand, two unique types were found in two and three patients respectively, raising the possibility of crossinfections.

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“…On completion of electrophoresis the cellulose acetate sheets were immersed in a solution of a-naphthyl acetate and fast blue salt for 30 min. (Lawrence, Melnick & Weimer, 1960). It was found that subsequent treatment of the stained sheet with 0.1 N-NaOH accentuated the colour of the dye in the areas of enzymic activity, which greatly facilitated the detection of weak esterase bands in the zymogram.…”

Section: Phaseolus Vulgarismentioning

confidence: 97%

Determination of Multiple Forms of Esterases in Rhizobium by Paper Electrophoresis

Murphy

Masterson

1970

Journal of General Microbiology

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SUMMARYFifty-two strains, comprising six Rhizobium species, were examined for their esterase patterns using electrophoresis on cellulose acetate. Esterase activity was detected in five Rhizobium species. The sixth species, R. japonicum, was characterized by the absence of esterase activity in all but one of the strains examined. Rhizobium trifolii and R. leguminosarum strains showed similarities in their esterase profiles. Rhizobium meliloti strains formed a group distinct from these on the basis of their esterase patterns. Rhizobium lotus sp. and R. phaseoli also exhibited esterase activity.Heat denaturation and metal inhibition studies suggest that the esterase activity is truly enzymic. The inability of the bacterial esterase to react with a synthetic peptide suggests that residual esterase activity associated with certain proteolytic enzymes is not involved. Heat tests revealed differences in the sensitivity of the multiple forms of esterases in rhizobia to inactivation.

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A Species Comparison of Serum Proteins and Enzymes by Starch Gel Electrophoresis.

Cited by 122 publications

References 1 publication

Isozyme variation and somatic incompatibility in populations of the ectomycorrhizal fungus Suillus collinitus

Isozyme variation and somatic incompatibility in populations of the ectomycorrhizal fungus Suillus collinitus

Complexity ofPseudomonas aeruginosainfection in cystic fibrosis: combined results from esterase electrophoresis and rDNA restriction fragment length polymorphism analysis

Determination of Multiple Forms of Esterases in Rhizobium by Paper Electrophoresis

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