A Specific Color Reaction of Methylpentoses and a Spectrophotometric Micromethod for Their Determination

Dische, Zacharias; Shettles, Landrum B.

doi:10.1016/s0021-9258(18)57178-7

Cited by 1,535 publications

(119 citation statements)

References 2 publications

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“…Fucoidan content in algal biomass. Since L-fucose is practically the only 6-deoxyhexose present in brown algae, the fucoidan content can be evaluated by determination of this sugar using specific color reaction for 6-deoxysugars (sulfuric acid + L-cystein hydrochloride [6]) or by chromatography after acid hydrolysis. The spectrophotometric procedure recommended [7] is inapplicable in many cases due to interference by colored substances extracting together with fucoidans from the biomass.…”

Section: Methods Of Structural Analysismentioning

confidence: 99%

Structural Analysis of Fucoidans

Bilan

Усов

2008

Natural Product Communications

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Sulfated polysaccharides of brown algae ("fucoidans") constitute a wide variety of biopolymers from simple sulfated fucans up to complex heteropolysaccharides composed of several neutral monosaccharides, uronic acid and sulfate. The increased interest in this class of polysaccharides is explained by their high and versatile biological activities, and hence, by their possible use in new drug design. Structural analysis of several fucoidans demonstrates that their biological properties are determined not only by charge density, but also by fine chemical structure, although distinct correlations between structure and biological activity cannot be formulated at present. The aim of this review is to describe the methods of structural analysis currently used in fucoidan chemistry, and to discuss some new information on the structures of fucoidans presented in recent publications.

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Section: Methods Of Structural Analysismentioning

confidence: 99%

Structural Analysis of Fucoidans

Bilan

Усов

2008

Natural Product Communications

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“…GMMA purity was assessed by HPLC-SEC analysis [45]; total protein content was estimated by micro BCA (Thermo Scientific, Waltham, MA, USA); O-Ag sugar content was quantified by determination of methyl pentoses (6-deoxyhexoses) with the Dische colorimetric method [46].…”

Section: Gmma Production and Characterizationmentioning

confidence: 99%

Conformational and Immunogenicity Studies of the Shigella flexneri Serogroup 6 O-Antigen: The Effect of O-Acetylation

Richardson

Ravenscroft

Arato³

et al. 2021

Vaccines

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The pathogenic bacterium Shigella is a leading cause of diarrheal disease and mortality, disproportionately affecting young children in low-income countries. The increasing prevalence of antibiotic resistance in Shigella necessitates an effective vaccine, for which the bacterial lipopolysaccharide O-antigen is the primary target. S. flexneri serotype 6 has been proposed as a multivalent vaccine component to ensure broad protection against Shigella. We have previously explored the conformations of S. flexneri O-antigens from serogroups Y, 2, 3, and 5 that share a common saccharide backbone (serotype Y). Here we consider serogroup 6, which is of particular interest because of an altered backbone repeat unit with non-stoichiometric O-acetylation, the antigenic and immunogenic importance of which have yet to be established. Our simulations show significant conformational changes in serogroup 6 relative to the serotype Y backbone. We further find that O-acetylation has little effect on conformation and hence may not be essential for the antigenicity of serotype 6. This is corroborated by an in vivo study in mice, using Generalized Modules for Membrane Antigens (GMMA) as O-antigen delivery systems, that shows that O-acetylation does not have an impact on the immune response elicited by the S. flexneri serotype 6 O-antigen.

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“…The liver tissues taken were homogenized (10% (w/v)) in ice-cold saline. From the liver tissue homogenates, hexose, hexosamine, and fucose levels were quantified by the methods of Winzler (17) and Dische and Shettles (18). For the sialic acid levels, the method of Lorentz et al was used (19).…”

Section: Biochemical Assaysmentioning

confidence: 99%

Diyabetik Sıçan Karaciğerinde Pazı Ekstresinin Glikoprotein Bileşikleri ve İleri Oksidasyon Protein Ürün Seviyeleri Üzerindeki Koruyucu Etkisi

Yanardağ¹

2021

Experimed

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A Specific Color Reaction of Methylpentoses and a Spectrophotometric Micromethod for Their Determination

Cited by 1,535 publications

References 2 publications

Structural Analysis of Fucoidans

Structural Analysis of Fucoidans

Conformational and Immunogenicity Studies of the Shigella flexneri Serogroup 6 O-Antigen: The Effect of O-Acetylation

Diyabetik Sıçan Karaciğerinde Pazı Ekstresinin Glikoprotein Bileşikleri ve İleri Oksidasyon Protein Ürün Seviyeleri Üzerindeki Koruyucu Etkisi

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