A spectroscopic and thermal investigation into the relationship between composition, secondary structure and physical characteristics of electrospun zein nanofibers

Wang, Y. H.; Zhao, Min; Barker, S. A.; Belton, Peter; Craig, Duncan Q.M.

doi:10.1016/j.msec.2018.12.134

Cited by 42 publications

(10 citation statements)

References 36 publications

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“…In another study, diluted acetic acid was employed and found to result in fibers only at low water concentrations, which showed a compact and dense structure [31]. Attempts to electrospin zein in a mixture of acetic acid, ethanol and water resulted in fibers with a beads-on-fiber morphology [32]. In addition to the challenges of electrospinning zein, the fabricated fibers tend to lose their porous structure and turn into a film upon contact with water [19,20,24,33].…”

Section: Introductionmentioning

confidence: 99%

“…There is only a limited number of studies that produced fibers from zein and PEO by electrospinning, and to the best of our knowledge there are no studies on electrospinning of mixtures of zein and SA. With respect to studies focusing on zein and PEO, zein was uniaxially electrospun with PEO [30,32], and co-axially electrospun with zein as a shell and PEO as a core [47]. However, in all cases high PEO concentrations and low zein concentrations were required, to improve the electrospinning and prevent ribbon morphology from occurring.…”

Section: Introductionmentioning

confidence: 99%

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Highly Elastic and Water Stable Zein Microfibers as a Potential Drug Delivery System for Wound Healing

et al. 2020

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The development of biomaterials for wound healing applications requires providing a number of properties, such as antimicrobial action, facilitation of cell proliferation, biocompatibility and biodegradability. The aim of the present study was to investigate morphological and mechanical properties of zein-based microfibers, ultimately aimed at creating an environment suitable for wound healing. This was achieved through co-axial electrospinning of core–shell microfibers, with zein protein in the core and polyethylene oxide (PEO) in the shell. Small amounts of PEO or stearic acid were additionally incorporated into the fiber core to modify the morphology and mechanical properties of zein fibers. The presence of PEO in the core was found to be essential for the formation of tubular fibers, whereas PEO in the shell enhanced the stability of the microfibers in water and ensured high elasticity of the microfiber mats. Tetracycline hydrochloride was present in an amorphous form within the fibers, and displayed a burst release as a result of pore-formation in the fibers. The developed systems exhibited antimicrobial activity against Staphylococcus aureus and Escherichia coli, and showed no cytotoxic effect on fibroblasts. Biocompatibility, antimicrobial activity and favorable morphological and mechanical properties make the developed zein-based microfibers a potential biomaterial for wound healing purposes.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Highly Elastic and Water Stable Zein Microfibers as a Potential Drug Delivery System for Wound Healing

et al. 2020

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“…The background spectrum was automatically subtracted from the spectra of the samples. Individual components within the spectra were estimated by second derivative and Lorentz curve fitting in the regions of 1,700–1,600 cm −1 using OriginPro 9.0 software (OriginLab, USA) 36,37 . The area under the curve of each deconvoluted band was calculated.…”

Section: Methodsmentioning

confidence: 99%

“…Individual components within the spectra were estimated by second derivative and Lorentz curve fitting in the regions of 1,700-1,600 cm À1 using OriginPro 9.0 software (OriginLab, USA). 36,37 The area under the curve of each deconvoluted band was calculated. All the deconvolutions were done with an R 2 of 0.99.…”

Section: Ftir Analyses Of Filmsmentioning

confidence: 99%

Incorporation of organic acids turns classically brittle zein films into flexible antimicrobial packaging materials

2021

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This study aimed to turn classically brittle zein films into flexible antimicrobial ones by the use of lactic (LA), malic (MA) and tartaric acids (TA). The most effective plasticizer was LA (400% elongation at break [EB] at 4%), while MA (189% EB at 4.5%) and TA (68% EB at 5%) showed moderate and limited plasticizing effects, respectively.The LA-and MA-loaded films maintained their flexibility during 30-day storage at 4 C or 25 C. Fourier transform infrared (FTIR) analysis suggested that the plasticization of LA and MA could be related to secondary structural changes in zein such as increased α-helix and random coils (mainly by MA) and spaced/modified intermolecular (only by LA) and intramolecular (mainly by MA) β-sheets. Atomic force and scanning electron microscopy showed that LA and MA gave more homogenous and smoother films than TA. Films with LA showed the highest water vapour permeability followed by those of control, MA-and TA-loaded films. Films with 3%-4% LA or MA formed clear zones on Listeria innocua and Klebsiella pneumonia, but only films with LA formed clear zones on Escherichia coli. All OA-loaded films gave unclear zones on Staphylococcus aureus in disc-diffusion tests, but this bacterium was inactivated rapidly in antimicrobial tests based on surface inoculation tests. LA is the best OA to develop flexible antimicrobial films from zein, an industrial by-product that films could not have been utilized as a widespread packaging material due to their brittleness.

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“…The methods of forming nanocarriers from zein include antisolvent (Cheng et al., 2019; Zhang et al., 2019), electrospinning (Deng, Li, Feng, Wu, & Zhang, 2019; Heydari‐Majd, Rezaeinia, Shadan, Ghorani, & Tucker, 2019; Wang, Zhao, Barker, Belton, & Craig, 2019), electrospraying (Miguel et al., 2019), supercritical antisolvent (Franco, Reverchon, & De Marco, 2019; Palazzo, Campardelli, Scognamiglio, & Reverchon, 2019), heat‐induced self‐assembly (Wang & Zhang, 2019), and evaporation induced self‐assembly (Wang, Su, Schulmerich, & Padua, 2013). Although these methods have different processes, they follow a common mechanism.…”

Section: Characteristics Of Zeinmentioning

confidence: 99%

Development of a nanoparticle delivery system based on zein/polysaccharide complexes

2020

Journal of Food Science

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Zein, an alcohol-soluble protein in corn, can self-assemble into spherical nanoparticles, which makes it an ideal carrier material for the encapsulation of hydrophobic bioactive molecules. However, zein nanoparticles easily aggregate in water and are sensitive to enzymatic degradation in the digestive system. A strategy to overcome their limitations is the incorporation of polysaccharides as a second polymer layer that provides stability to zein nanoparticles. This review introduces the characteristics of zein as they relate to understanding the formation of zein/polysaccharide nanoparticles. Particular attention is paid to the preparation methods for the zein/polysaccharide nanoparticles, as well as to the morphological observation methods and detection mechanism. Moreover, the properties and applications of zein/polysaccharide nanoparticles are highlighted.

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A spectroscopic and thermal investigation into the relationship between composition, secondary structure and physical characteristics of electrospun zein nanofibers

Abstract: The version in the Kent Academic Repository may differ from the final published version. Users are advised to check http://kar.kent.ac.uk for the status of the paper. Users should always cite the published version of record.

Cited by 42 publications

References 36 publications

Highly Elastic and Water Stable Zein Microfibers as a Potential Drug Delivery System for Wound Healing

Highly Elastic and Water Stable Zein Microfibers as a Potential Drug Delivery System for Wound Healing

Incorporation of organic acids turns classically brittle zein films into flexible antimicrobial packaging materials

Development of a nanoparticle delivery system based on zein/polysaccharide complexes

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