In this paper, multiagent tracking control problem of second-order multiagent systems with unknown leader acceleration, input saturation, and matched and unmatched disturbances is investigated. An auxiliary system is constructed to approximate system position states, and a novel sliding mode disturbance observer is designed to estimate matched and unmatched uncertainties. A sliding mode disturbance observer-based control protocol is proposed by constructing a novel sliding mode manifold based on the sliding mode disturbance observer outputs. In addition, the input saturation and the unknown leader acceleration become a part of lumped uncertainties by using mathematic transformation. The lumped uncertainties estimated by the sliding mode disturbance observer are compensated by the sliding mode disturbance observer-based control protocol. Stability of the second-order multiagent systems is guaranteed via Lyapunov method. Finally, a simulation example is proposed to exhibit advantages and availability of the developed techniques.