A Stability-Indicating High Performance Liquid Chromatographic Method For the Determination of Nelarabine

Vidyadhara, S.; Sasidhar, R. L. C.; Rao, Ballipalli Venkateswara; Saibabu, T.; Harika, D. Lakshmi

doi:10.13005/ojc/320168

Cited by 3 publications

(3 citation statements)

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“…Since there is no standardization of tempeh production, the ethanolic extract of tempeh was standardized by analyzing the concentration of genistein in the corresponding extract [12][13][14] . Genistein in soy has been accurately analyzed using reverse phase HPLC method [12][13][14]19,26 , which was therefore applied here to determine genistein concentration in the ethanolic extract.…”

Section: Resultsmentioning

confidence: 99%

“…Chromatography was carried out using methanol-redistilled water 70:10 (v/v) as the mobile phase. The flow rate of the mobile phase was maintained at 0.7 mL/min in the isocratic reversephase HPLC system 19 . The injection volume was 10 µL.…”

Section: Preparation Of Ethanolic Extract Of Tempeh and Determinationmentioning

confidence: 99%

“…The preparation of ethanolic extract of tempeh and the subsequently standardization of the extract by analyzing the concentration of phytochemical genistein are presented in this article 13,14,[17][18][19] . The cytotoxic activity on T47D cells of the genistein-standardized ethanolic extract of tempeh was evaluated and compared to genistein as the reference compound 20,21 .…”

Section: Introductionmentioning

confidence: 99%

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The Cytotoxic Activity on T47D Breast Cancer Cell of Genistein -Standardized Ethanolic Extract of tempeh -A Fermented Product of Soybean ( Glycine Max)

Yuliani¹,

Istyastono²,

Riswanto³

2016

Orient. J. Chem

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As fermented soybean (Glycine max), tempeh has been reported as a good source of isoflavones, which have shown anticancer activities in various cancer cells resulted from their activity as phytoestrogens. In this article, the preparation of ethanolic extract of tempeh is presented and then followed by the exploration of its cytotoxic effect on T47D cells. The extract was subsequently standardized using genistein, the major phytoestrogen found in tempeh. The genistein concentration found in the extract of tempeh was 0.681 % (w/w). The cytotoxicity assay employing 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) resulted in IC 50 values of 196.066 ± 15.956 µg/mL and 13.174 ± 0.905 µg/mL for the genistein-standardized ethanolic extract of tempeh and genistein, respectively.

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Section: Resultsmentioning

confidence: 99%

Section: Preparation Of Ethanolic Extract Of Tempeh and Determinationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Cytotoxic Activity on T47D Breast Cancer Cell of Genistein -Standardized Ethanolic Extract of tempeh -A Fermented Product of Soybean ( Glycine Max)

Yuliani¹,

Istyastono²,

Riswanto³

2016

Orient. J. Chem

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Identification, isolation, and structure elucidation of novel forced degradation products of nelarabine

Parmar

Rajput

Mohan

2023

Separation Science Plus

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The present study focuses on novel degradation products of nelarabine generated from forced degradation study. In the process, a novel analytical method was developed which was not only compatible with liquid chromatography‐mass spectrometry but also had the efficiency to capture each of the possible degradation products with sufficient separation from the nelarabine peak. Nelarabine was exposed to acidic, alkali, oxidation, photolytic, and thermal stress conditions as recommended by International Conference on Harmonization guidelines. No major degradation was observed under photolytic and thermal conditions however the drug was observed to be significantly degraded under acidic, alkali, and oxidative stress conditions generating a total of nine novel degradation products which are hitherto not been reported in the literature. The major degradation products obtained under acidic and alkali conditions were isolated using preparative high‐performance liquid chromatography and their structure was elucidated using nuclear magnetic resonance spectroscopy and high‐resolution mass spectroscopic studies. Moreover, probable pathways for the formation of these degradation products were also outlined.

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Purine-based anticancer drugs

Elgemeie,

Mohamed-Ezzat

2022

New Strategies Targeting Cancer Metabolism

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A Stability-Indicating High Performance Liquid Chromatographic Method For the Determination of Nelarabine

Cited by 3 publications

References 1 publication

The Cytotoxic Activity on T47D Breast Cancer Cell of Genistein -Standardized Ethanolic Extract of tempeh -A Fermented Product of Soybean ( Glycine Max)

The Cytotoxic Activity on T47D Breast Cancer Cell of Genistein -Standardized Ethanolic Extract of tempeh -A Fermented Product of Soybean ( Glycine Max)

Identification, isolation, and structure elucidation of novel forced degradation products of nelarabine

Purine-based anticancer drugs

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