The Cytotoxic Activity on T47D Breast Cancer Cell of Genistein -Standardized Ethanolic Extract of tempeh -A Fermented Product of Soybean ( Glycine Max)

Abstract

As fermented soybean (Glycine max), tempeh has been reported as a good source of isoflavones, which have shown anticancer activities in various cancer cells resulted from their activity as phytoestrogens. In this article, the preparation of ethanolic extract of tempeh is presented and then followed by the exploration of its cytotoxic effect on T47D cells. The extract was subsequently standardized using genistein, the major phytoestrogen found in tempeh. The genistein concentration found in the extract of tempeh was 0.681 % (w/w). The cytotoxicity assay employing 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) resulted in IC 50 values of 196.066 ± 15.956 µg/mL and 13.174 ± 0.905 µg/mL for the genistein-standardized ethanolic extract of tempeh and genistein, respectively.

Development of a Graphical User Interface Application to Identify Marginal and Potent Ligands for Estrogen Receptor Alpha

“…The development of GUI application was performed on a Linux (Linux Mint 18.3 Cinnamon 64-bit) machine with Intel © Dual Core™ i5-6200UCPU E31220 (@ 2.30 GHz) as the processors and 4.00 GB of RAM using the same software to perform SBVS campaigns appended by the following software to create a GUI application: Zenity 3.18.1.1 (http://live.gnome.org/Zenity) and BKChem 0.13.0 (http://bkchem.zirael.org/). The materials for performing the in vitro tests were the same as the materials presented in [19]. Genistein, genistin, daidzein, and daidzin as the in vitro test compounds were purchased from Sigma-Aldrich.…”

“…The developed GUI application was then employed to virtually screen genistein, genistin, daidzein, and daidzin. The in vitro test procedures were the same as the procedures employed by [19] and were performed in the Integrated Research and Assessment Laboratory, Gadjah Mada University, Yogyakarta. The IC50 values were calculated using two parameters logistic regressions in R-3.3.0 [15,[25][26].…”

“…The application was then employed to virtually screen genistin, genistein, daidzin, and daidzein. The results were subsequently compared to the results from in vitro tests employing cytotoxic assay on T47D cell line using 3-[4,5dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) method [15,19].…”

Employing an R Software Package rsm for Optimizing of Genistein, Daidzein, and Glycitein Separation and Its Application for Soy Milk Analysis by HPLC Method

“…It was stated that soy foods were not only providing highquality protein and healthful fat but also having health benefits such as reducing the risk of coronary heart disease, performing an anticancer activity for breast and prostate cancer, improving renal function, brain smartness, and skin health [2][3]. In the previous studies [4][5][6], several biological activities of soy foods indicated the presence of isoflavones content as bioactive compounds.…”

Effects of Particle Size, Extraction Time, and Solvent Selection on Daidzein Extracted Amount from Tempeh-A Fermented Product of Soybean

“…Daidzein, an isoflavone aglycone, was reported as the major compound contained in soybeans (Kuligowski et al 2016). In recent years, pharmacological activities of daidzein were reported such as anti-breast cancer agent (Liu et al 2012, Yuliani et al 2016, cell growth inhibitor (Takaoka et al 2018), antidiabetic agent, (Park et al 2013), antiinflammatory agent, anti-oxidant agent (Peng et al 2017), and matrix metalloproteinase-9 suppressor (Oh et al 2013). Tempeh is a fermented product of soybean and commonly used as Indonesian traditional food with high isoflavones content (Yuliani et al, 2018).…”