A stable liquid–liquid extraction system for clavulanic acid using polymer-based aqueous two-phase systems

Abstract

a b s t r a c tThe partitioning of Clavulanic Acid (CA) in a novel inexpensive and stable aqueous two-phase system (ATPS) composed by poly(ethylene glycol) (PEG) and sodium polyacrylate (NaPA) has been studied. The aqueous two-phase systems are formed by mixing both polymers with a salt (NaCl or Na 2 SO 4 ) and an aqueous solution of CA. The stability of CA on the presence of both polymers was investigated and it was observed that these polymers do not degrade the biomolecule. The effect of PEG-molecular size, polymer concentrations on the commercial CA partitioning has been studied, at 25°C. The data showed that commercial CA was preferentially partitioned for the PEG-rich phase with a partition coefficient (K CA ) between 1 and 12 in the PEG/NaPA aqueous two phase systems supplemented with NaCl and Na 2 SO 4 . The partition to the PEG phase was increased in the systems with high polymer concentrations. Furthermore, Na 2 SO 4 caused higher CA preference for the PEG-phase than NaCl. The systems having a composition with 10 wt.% of PEG4000, 20 wt.% of NaPA8000 and 6 wt.% of Na 2 SO 4 were selected as the optimal ones in terms of recovery of CA from fermented broth of Streptomyces clavuligerus. The partitioning results (K CA = 9.15 ± 1.06) are competitive with commercial extraction methods of CA (K CA = 11.91 ± 2.08) which emphasizes that the system PEG/NaPA/Na 2 SO 4 can be used as a new process to CA purification/concentration from fermented broth.

Extraction of natural red colorants from the fermented broth ofPenicillium purpurogenumusing aqueous two-phase polymer systems

“…Since ATPS is a technique of quick and easy application several studies to purify and separate contaminants of the target molecules has been performed. Among the molecules studied employing ATPS formed by two polymers or a polymer and a salt can be cited: oxidase ascorbate, proteases, bromelain, laccases, betalain, lutein, polyketides, collagenase, and antibiotics …”

Study on extraction and separation of butyric acid from clostridium tyrobutyricum fermentation broth in PEG/Na2SO4 aqueous two-phase system

“…Specifically, sodium sulfate saturated solution was employed to precipitate carbohydrate and nitrogen compounds in clostridium tyrobutyricum fermentation broth, followed by adding polyethylene glycol (PEG)/sodium sulfate ATPS for butyric acid extraction from the broth. The ATPS method is characterized by low energy consumption and possibility of reusing the extraction agents [6][7][8][9][10][11]. We investigated the effects of different ATPSs on the extraction yield of butyric acid, and systematically studied the extraction conditions by a single factor and response surface analysis (RSA), so as to obtain a new process for the efficient extraction of butyric acid from a fermentation broth.…”

Obtenção De Dados De Equilíbrio De Sistemas Aquosos Bifásicos Formados Por Polietilenoglicol E Líquido Iônico

“…Esta técnica é amplamente utilizada na purificação de biomoléculas, onde as suas vantagens incluem seletividade favorável, baixo custo e adaptabilidade para o processamento contínuo da amostra e retenção da atividade biológica (KRONER et al, 1978). SAB a base de polímeros têm sido largamente utilizados na separação e purificação de proteínas, enzimas, células do sangue e antibióticos (ALBERTSSON, 1986;ZASLAVSKY, 1995;PEREIRA et al, 2012). Entretanto, apesar de suas vantagens, SAB à base de polímeros apresentam fases com polaridades semelhantes, um fato que restringe sua aplicação, devido a baixos rendimentos de extração e/ou purificação.…”

“…As diferenças limitadas entre as duas fases nestes sistemas convencionais, conduziu à procura de estratégias alternativas, tais como a derivatização do polímero ou a utilização de aditivos, a fim de melhorar os rendimentos de extração da molécula alvo (LI e KAO, 2003;ROSA et al, 2007;JIANG et al, 2009;PEREIRA et al, 2010).…”