A stainless steel multi‐well plate (SS‐MWP) for high‐throughput Raman analysis of dilute solutions

Abstract

The use of Raman spectroscopy for the qualitative and quantitative analysis of dilute aqueous solutions is of interest to the biopharmaceutical manufacturing sector. However, the inherent weakness of the Raman effect, coupled with spectral variability due to spurious signals from sample holders, can produce significant problems for chemometric‐based high‐throughput assays. Therefore, there is a need for a multi‐well sample holder that ensures robust and repeatable measurements, in particular from dilute aqueous solutions such as cell culture media. Here we demonstrate the efficacy of a novel, electropolished, stainless steel multi‐well plate (SS‐MWP) sample holder with 96 wells for dilute aqueous solution analysis. A comprehensive study of the spectroscopic behaviour was carried out and comparisons made with multi‐well plates fabricated from polystyrene, polypropylene, and aluminium. A key factor in the validation studies is the use of intrinsically weak Raman scattering systems, e.g. water and dilute glucose solutions. The data collected show that the SS‐MWPs are much superior in terms of robustness, resistance to chemical attack, and measurement reproducibility and as such are the ideal sample holders for Raman analysis of dilute solutions. Copyright © 2010 John Wiley & Sons, Ltd.

Recent advances in linear and nonlinear Raman spectroscopy. Part V

“…Ryder et al . demonstrated the efficacy of a novel, electropolished, stainless steel multi‐well plate sample holder with 96 wells for dilute aqueous solutions analysis and carried out a comprehensive study of its Raman spectroscopic behavior and comparisons with multi‐well plates fabricated from polystyrene, polypropylene and aluminum . A short 16‐amino acid peptide was used by Ryu et al .…”

Applications of Raman Spectroscopy in Biopharmaceutical Manufacturing: A Short Review

“…This is not a trivial undertaking as many of the biogenic samples generated by BioPharma are biologically, chemically, and physically unstable and even apparently minor differences in sample handling (e.g., stirred not shaken) can, and will, cause major differences in measurements for all techniques. 28,32,[34][35][36]58,59,73,118,131 Sample Handling A very important issue in BioPharma concerns the physical nature of the sample and whether one is testing solids or liquids. Solid-state cell culture media, for example, are complex powders (e.g., media, hydrolysates, etc.)…”

Rapid quantification of tryptophan and tyrosine in chemically defined cell culture media using fluorescence spectroscopy

“…10 This can make routine analysis expensive and time-consuming particularly for simple 11 requirements such as raw materials or media constituent identification, determination of lot-12 to-lot consistency, and the monitoring of batch-to-batch preparations of media. Thus there is 13 a need for a rapid, holistic analytical method that can be used for the early stage screening 14 and analysis of these materials.…”

“…Spectroscopic methods offer the ideal solution because of 15 advantages ranging from speed, sensitivity, facile automation, and inexpensive unit test costs. 16 Raman spectroscopy can be used for the identification and quality monitoring of cell culture 17 media components including eRDF [10,11]. However, quantification of specific components 18 is difficult because the analyte signals are typically weak and there may be fluorescent 19 interferences.…”

“…10 As the goal of this study is to determine the concentrations of specific amino acids in a 11 complex cell culture media, we used a variety of methods. applications are expected to be lower than that used here because the media are supposed to 13 be manufactured to a specific recipe with very high tolerances.…”