A standard haemagglutination inhibition test for Newcastle disease. (2) Vaccination and challenge

Allan, W. H.; Gough, R. E.

doi:10.1136/vr.95.7.147

Cited by 61 publications

(34 citation statements)

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“…However, the relationship between HI antibody level and protection seems to depend on a number of factors, namely, the vaccine strain, the route of administration and the virulence of the challenge virus. Allan and Gough (1974) observed that La Sota vaccine is associated with a higher death rate than B1 vaccine at comparable HI antibody levels, thus confirming that HI antibody/velogenic virus challenge relationship may vary between strains. However, death rate appears to be inversely related to the flock HI antibody levels; and log 2 HI antibody titer of 3 has long been widely accepted as protective against ND mortality (Allan and Gough, 1974;Spradbrow et al, 1980;Jagne et al, 1991).…”

Section: Introductionmentioning

confidence: 56%

Relationship between antibody titers from V4-in-cassava vaccination and protection against velogenic virus challenge in laboratory chickens

Iroegbu¹,

Okeke²,

Ike³

2014

Afr. J. Microbiol. Res.

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Unvaccinated laboratory-raised, five week old cockerels were fed V 4 newcastle disease vaccine in cassava once, twice or thrice; and tested for hemagglutination inhibition (HI) antibody response. They were subsequently challenged with a local isolate of velogenic newcastle disease virus (NDV). Immune status of the chicken flocks improved with number of vaccinations. Survival from velogenic virus challenge varied significantly with pre-challenge HI antibody titer, protection being better at log 2 titer of ≥3 than at log 2 titers of ≤ 2 but no difference was observed between titers of 3, 4 and 5. Although HI log 2 antibody titers of 3 and 4 were apparently observed to protect vaccinated chickens against ND mortality in this work, 7 out of 8 (87.5%) of the chickens that manifested torticollis (neurological disease symptom) had pre-challenge HI antibody titers within this range. Thus, it is suggested that while log 2 HI antibody titer of 3 may be taken as cut-off point for sero-conversion, titers of 5 and above may protect against neurological symptoms.

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Section: Introductionmentioning

confidence: 56%

Relationship between antibody titers from V4-in-cassava vaccination and protection against velogenic virus challenge in laboratory chickens

Iroegbu¹,

Okeke²,

Ike³

2014

Afr. J. Microbiol. Res.

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“…Ancak, sanayi kolu haline gelen tavuk yetiştiriciliği için, aşı lamaların, hayvanları hırpalamadan, kısa sürede uygulanabilen ve verime etkilerneden yapılması çok önemlidir. Bu nedenlerle de zaman kaybına yol açan hayvanları elle teker teker tutup aşılamak yerine, aşı virusunu sularına katarak veya aerosol aşılama yöntemleri pratiğe konulmuştur (1,3,5,9,10,14,22,26 Bu araştırmanın sonucuna göre, yeterli düzeyde maternal antikor içermeyen ha.yvanların LaSota içme suyu ile aşılanmaları halinde aşı-ya ilgili bir stres görülmeyeceği ve bu aşılamanın hayvanlarda yeterli ve kalıcı bir bağışıklık sağlayacağı ve HI titreleri ile bağışıklık testleri arasında bir korelasyonun bulunduğu kanısına varılmıştır.…”

Section: Tartışma ' C Sonuçunclassified

NEWCASTLE HASTAllGINA KARŞI AŞıLAMA DENEMELERİ

Birsen;AKAY¹

1984

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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“…The serum samples were tested for ND antibody titres to establish the pre-vaccination sero-status using the Haemagglutination Inhibition (HI) test [13]. The serum samples were stored at 4˚C before testing for protection against ND by the HI test [13]. Virus isolation was further carried out from cloacal, trachea, spleen, lung, liver and heart following post mortem from suspected suspected ND cases.…”

Section: Methodsmentioning

confidence: 99%

“…Serum samples representing 100%, were collected from all chicken, using the wing veins. The serum samples were tested for ND antibody titres to establish the pre-vaccination sero-status using the Haemagglutination Inhibition (HI) test [13]. The serum samples were stored at 4˚C before testing for protection against ND by the HI test [13].…”

Section: Methodsmentioning

confidence: 99%

Newcastle Disease Virus Isolation and Its Prevalence in Uganda Poultry Farms

Kasozi¹,

Ssuna²,

Tayebwa³

et al. 2014

OJVM

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The present research work was carried out to isolate and identify Newcastle disease virus (NDV) by using haemagglutination inhibition (HI) test and HA-HI virus isolation, embryonated eggs (EE) and chicken embryo fibroblasts (CEF).A total of 95 clinical (blood, tracheal and cloacal swabs) and post-mortem (brain, lung, colon and spleen) samples were collected from chickens of field outbreaks of suspected Newcastle disease virus (NDV). The HI and HA-HI were employed to detect NDV in tissue homogenates of all the clinical and post-mortem samples as well as laboratory samples (AF and ICF). Among the four different types of post-mortem samples, virus isolation rate was found to be low in body organs. In CEF cell culture system, the rate of virus isolation from all the aforesaid samples was found to be at 100% with the exception of serum samples; while in tracheal and cloacal swabs, it was at 90%; while in serum, it was at 10%, in all clinical cases. The isolation rate of NDV was higher in CEF culture system (66.7%) compared to that of avian embryos (33.3%). Samples were inoculated and the allantoic fluid (AF) of the dead embryos and the infected culture fluid (ICF) of the CEF were harvested at 24 to 96 hours of the post-infection, respectively, which revealed that the virulent strain of NDV is highly prevalent in the region. The prevalence of NDV was established at 1.1%, 2.1% and 4.2% using HA-HI, EE, and CEF methods. Rapid detection and identification of the virus are crucial for the effective control of the disease as conventional diagnostic methods such as virus isolation on embryonated eggs followed by serological identification in haemagglutination-inhibition test are laborious and time-consuming. The speed of the diagnosis can be considerably increased by using methods based on molecular biology, e.g. reverse transcription-polymerase chain reaction. However, the genetic variability of APMV-1 isolates should be considered carefully as the potential cause for false negative results of genetic-based laboratory tests.

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A standard haemagglutination inhibition test for Newcastle disease. (2) Vaccination and challenge

Cited by 61 publications

References 0 publications

Relationship between antibody titers from V4-in-cassava vaccination and protection against velogenic virus challenge in laboratory chickens

Relationship between antibody titers from V4-in-cassava vaccination and protection against velogenic virus challenge in laboratory chickens

NEWCASTLE HASTAllGINA KARŞI AŞıLAMA DENEMELERİ

Newcastle Disease Virus Isolation and Its Prevalence in Uganda Poultry Farms

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