A standardization of the Novelty-Suppressed Feeding Test protocol in rats

Blasco‐Serra, Arantxa; González‐Soler, Eva M.; Cervera‐Ferri, Ana; Teruel‐Martí, Vicent; Valverde‐Navarro, Alfonso A.

doi:10.1016/j.neulet.2017.08.019

Cited by 90 publications

(39 citation statements)

References 35 publications

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“…Our initial analysis of mGAL5.1KO mice using the MBT demonstrated that removal of mGAL5.1 increased the tendency of both male and female mice lacking GAL5.1 to bury marbles; an accepted diagnostic of anxiety-like behaviour but also of obsessive compulsive disorder 41 . Interestingly, we also observed greatly increased numbers of line crossing as well as more distance travelled by both sexes of mGAL5.1 mice exposed the NSFT (AKA hyponeophagia) which suggests that GAL5.1 may play a role in exploratory foraging behaviour 42 . However, we only observed a decrease in feeding latency in the NSFT in males lacking GAL5.1 which suggested the possibility that some aspects of anxiety-like behaviour in mice are modulated by GAL5.1 in a sex specific manner.…”

Section: Discussionmentioning

confidence: 70%

CRISPR disruption and UK Biobank analysis of a highly conserved polymorphic enhancer suggests a role in male anxiety and ethanol intake

et al. 2020

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Section: Discussionmentioning

confidence: 70%

CRISPR disruption and UK Biobank analysis of a highly conserved polymorphic enhancer suggests a role in male anxiety and ethanol intake

et al. 2020

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“…They were conducted to study locomotion in a non-familiar environment and anxiety-like behavior, respectively (Bodnoff et al, 1988; Seibenhener and Wooten, 2015; Blasco-Serra et al, 2017). Pigs were individually led to the waiting area adjacent to the arena (3.5 × 3.5 m).…”

Section: Methodsmentioning

confidence: 99%

Validation of a Psychosocial Chronic Stress Model in the Pig Using a Multidisciplinary Approach at the Gut-Brain and Behavior Levels

Menneson

Ménicot²,

Ferret‐Bernard³

et al. 2019

Front. Behav. Neurosci.

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Psychological chronic stress is an important risk factor for major depressive disorder, of which consequences have been widely studied in rodent models. This work aimed at describing a pig model of chronic stress based on social isolation, environmental impoverishment and unpredictability. Three groups of animals of both sexes were constituted. Two were exposed to the psychosocial stressors while receiving (SF, n = 12) or not (SC, n = 22) the antidepressant fluoxetine, and a third group (NSC, n = 22) remained unstressed. Animals were observed in home pens and during dedicated tests to assess resignation and anxiety-like behaviors. Brain structure and function were evaluated via proton MRS and fMRI. Hippocampal molecular biology and immunodetection of cellular proliferation (Ki67 + ) and neuron maturation (DCX + ) in the dentate gyrus were also performed. Salivary cortisol, fecal short-chain fatty acids (SCFAs), and various plasmatic and intestinal biomarkers were analyzed. Compared to NSC, SC animals showed more resignation ( p = 0.019) and had a higher level of salivary cortisol ( p = 0.020). SC brain responses to stimulation by a novel odor were lower, similarly to their hippocampal neuronal density ( p = 0.015), cellular proliferation ( p = 0.030), and hippocampal levels of BDNF and 5-HT 1A R ( p = 0.056 and p = 0.007, respectively). However, the number of DCX + cells was higher in the ventral dentate gyrus in this group ( p = 0.025). In addition, HOMA-IR was also higher ( p < 0.001) and microbiota fermentation activity was lower (SCFAs, SC/NSC: p < 0.01) in SC animals. Fluoxetine partially or totally reversed several of these effects. Exposure to psychosocial stressors in the pig model induced effects consistent with the human and rodent literature, including resignation behavior and alterations of the HPA axis and hippocampus. This model opens the way to innovative translational research exploring the mechanisms of chronic stress and testing intervention strategies with good face validity related to human.

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“…Novelty-suppressed feeding is widely used to assess anxiety and screen novel anti-depressants [25]. This test measures hyponeophagia, inhibition of feeding in response to a novel environment.…”

Section: Novelty-suppressed Feedingmentioning

confidence: 99%

Systemic conditioned medium treatment from interleukin-1 primed mesenchymal stem cells promotes recovery after stroke

et al. 2020

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Background: Mesenchymal stem cells (MSCs) hold great potential as a therapy for stroke and have previously been shown to promote recovery in preclinical models of cerebral ischaemia. MSCs secrete a wide range of growth factors, chemokines, cytokines and extracellular vesicles-collectively termed the secretome. In this study, we assessed for the first time the efficacy of the IL-1α-primed MSC-derived secretome on brain injury and functional recovery after cerebral ischaemia. Methods: Stroke was induced in male C57BL/6 mice using the intraluminal filament model of middle cerebral artery occlusion. Conditioned medium from IL-1α-primed MSCs or vehicle was administered at the time of reperfusion or at 24 h post-stroke by subcutaneous injection. Results: IL-1α-primed MSC-derived conditioned medium treatment at the time of stroke led to a~30% reduction in lesion volume at 48 h and was associated with modest improvements in body mass gain, 28-point neurological score and nest building. Administration of MSC-derived conditioned medium at 24 h post-stroke led to improved nest building and neurological score despite no observed differences in lesion volume at day 2 post-stroke. Conclusions: Our results show for the first time that the administration of conditioned medium from IL-1α-primed MSCs leads to improvements in behavioural outcomes independently of neuroprotection.

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A standardization of the Novelty-Suppressed Feeding Test protocol in rats

Cited by 90 publications

References 35 publications

CRISPR disruption and UK Biobank analysis of a highly conserved polymorphic enhancer suggests a role in male anxiety and ethanol intake

CRISPR disruption and UK Biobank analysis of a highly conserved polymorphic enhancer suggests a role in male anxiety and ethanol intake

Validation of a Psychosocial Chronic Stress Model in the Pig Using a Multidisciplinary Approach at the Gut-Brain and Behavior Levels

Systemic conditioned medium treatment from interleukin-1 primed mesenchymal stem cells promotes recovery after stroke

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