A standardized parameter for the distribution of individual Red Blood Cell deformability with the Cell Transit Analyzer

Amoussou-Guenou, K.M.; Squitiero, B.; Voutay, M.; Labat, B.; Rusch, Ph.

doi:10.3233/thc-1997-5502

Cited by 1 publication

(1 citation statement)

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“…In the current study, we not only looked at the deformability index but also at the baseline size and morphology of single red blood cell in patients with T2DM in comparison to controls. This is the first study in the literature reporting about the deformability characteristics of RBCs in T2DM using dual beam optical tweezers though there are previously reported studies using cell transit analyser 56 . The latest version of cell transit analyser is able to measure deformability of more than 1000 cells at the same time but it has 2% total sampling error as there can be some haemolysed RBCs or small size RBCs filtered through the micropores and giving a falsely high and possibly erroneous deformable rate for RBCs 56 .…”

Section: Discussionmentioning

confidence: 99%

Assessment of red blood cell deformability in type 2 diabetes mellitus and diabetic retinopathy by dual optical tweezers stretching technique

Agrawal

Smart

Cardoso

et al. 2016

Sci Rep

170

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A pilot cross sectional study was conducted to investigate the role of red blood cells (RBC) deformability in type 2 diabetes mellitus (T2DM) without and with diabetic retinopathy (DR) using a dual optical tweezers stretching technique. A dual optical tweezers was made by splitting and recombining a single Nd:YAG laser beam. RBCs were trapped directly (i.e., without microbead handles) in the dual optical tweezers where they were observed to adopt a “side-on” orientation. RBC initial and final lengths after stretching were measured by digital video microscopy, and a Deformability index (DI) calculated. Blood from 8 healthy controls, 5 T2DM and 7 DR patients with respective mean age of 52.4yrs, 51.6 yrs and 52 yrs was analysed. Initial average length of RBCs for control group was 8.45 ± 0.25 μm, 8.68 ± 0.49 μm for DM RBCs and 8.82 ± 0.32 μm for DR RBCs (p < 0.001). The DI for control group was 0.0698 ± 0.0224, and that for DM RBCs was 0.0645 ± 0.03 and 0.0635 ± 0.028 (p < 0.001) for DR group. DI was inversely related to basal length of RBCs (p = 0.02). DI of RBC from DM and DR patients was significantly lower in comparison with normal healthy controls. A dual optical tweezers method can hence be reliably used to assess RBC deformability.

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Section: Discussionmentioning

confidence: 99%