A stationary pseudophase semi-permanent coating for open-tubular capillary liquid chromatography and electrochromatography

Chiral separation using cyclodextrins as mobile phase additives in open‐tubular liquid chromatography with a pseudophase coating

“…Using β‐CD, γ‐CD, HP‐β‐CD, and CM‐β‐CD in the mobile phase with [CD] = 0.1 mM, chiral separation was observed for dichlorprop, mecoprop, ibuprofen, and ketoprofen, with CM‐β‐CD demonstrating the best chiral separation for all four analytes. The advantage of our method is that it is cheap and simple to prepare, and does not experience clogging from back pressure [ 25 ]. Also, the method uses small amounts of reagents and produces small amounts of waste.…”

“…The PDDA‐PSS‐coated capillaries were conditioned prior to each run as follows: 25 mM sodium tetraborate (pH 9.2) (3 capillary volumes), 0.5 mM DDAB in 25 mM sodium tetraborate (pH 9.2) (12 capillary volumes), 25 mM sodium tetraborate (pH 9.2) (3 capillary volumes), and mobile phase (10 capillary volumes). New capillaries were prepared each day as recommended in our previous study [ 25 ].…”

“…OT‐LC separations may offer advantages such as low sample and consumable requirements, zero backflow, and no column clogging. We have been exploring surfactant aggregates as pseudophase coatings in OT‐LC [ 23 , 24 , 25 ]. In particular, the double‐chained surfactant didodecyldimethyl ammonium bromide (DDAB) was shown as a semipermanent stationary pseudophase in OT‐LC [ 25 ].…”

“…We have been exploring surfactant aggregates as pseudophase coatings in OT‐LC [ 23 , 24 , 25 ]. In particular, the double‐chained surfactant didodecyldimethyl ammonium bromide (DDAB) was shown as a semipermanent stationary pseudophase in OT‐LC [ 25 ]. Unlike the use of long‐chain ionic surfactants (e.g., cetyltrimethylammonium bromide), no surfactant (i.e., DDAB) was added in the mobile phase [ 26 ].…”

Preparation of open tubular capillary column covalently coated with polystyrene sulfonate with 4,4′‐Azobis(4‐cyanopentanoyl chloride) as polymerization initiator for electrochromatographic separation of alkaloids, sulfonamides, and peptides

“…This hybrid technique was first introduced by Pretorius et al in 1974 for the analysis of nonpolar molecules [4]. Till now, various types of columns such as packed, monolithic, or open tubular (OT) with specific separation mechanisms like RP, ion exchange, hydrophilic partitioning, stereo-isomer selective, and ligand affinity can be fabricated [5][6][7][8][9][10]. The separation of solutes in CEC occurs by a combination of differential electrophoretic mobility and solutes-stationary-phase interaction [4].…”